scholarly journals The Biological Effects of Ozone Gas on Soft and Hard Dental Tissues and the Impact on Human Gingival Fibroblasts and Gingival Keratinocytes

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1978
Author(s):  
Alin Daniel Floare ◽  
Alexandra Denisa Scurtu ◽  
Octavia Iulia Balean ◽  
Doina Chioran ◽  
Roxana Buzatu ◽  
...  
Keyword(s):  
Biological Effects ◽  
Human Gingival Fibroblasts ◽  
Exclusion Criterion ◽  
Gingival Fibroblasts ◽  
Ozone Therapy ◽  
Dental Tissues ◽  
Diagnodent Pen ◽  
Occlusal Surfaces ◽  
Gingival Cells ◽  
The Impact

Ozone is an allotropic form of oxygen, so in the medical field ozone therapy has special effects. Starting from the premise that bio-oxidative ozone therapy reduces the number of bacteria, in the present study two approaches were proposed: to evaluate the biological effects of ozone gas on the tooth enamel remineralization process and to demonstrate its impact on the morphology and confluence of human primary gingival cells, namely keratinocytes (PGK) and fibroblasts (HGF). The ozone produced by HealOzone was applied in vivo to 68 M1s (first permanent molars), both maxillary and mandibular, on the occlusal surfaces at pit and fissure. The molars included in the study recorded values between 13 and 24 according to the DIAGNOdent Pen 2190 scale, this being the main inclusion/exclusion criterion for the investigated molars. Because the gas can make contact with primary gingival cells during the ozonation process, both human gingival fibroblasts and keratinocytes were exposed to different doses of ozone (20 s, 40 s, 60 s), and its effects were observed with the Olympus IX73 inverted microscope. The contact of ozone with the human primary gingival cells demonstrates cell sensitivity to the action of ozone, this being higher in fibroblasts compared to keratinocytes, but it is not considered toxic because all the changes are reversible at 48 h after exposure.

Biological effects of Er:YAG laser irradiation on the proliferation of primary human gingival fibroblasts

2017 ◽  
Vol 11 (3) ◽  
pp. e201700157 ◽  
Author(s):  
Sophannary Kong ◽  
Akira Aoki ◽  
Kengo Iwasaki ◽  
Koji Mizutani ◽  
Sayaka Katagiri ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Er:Yag Laser ◽  
Biological Effects ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts

Comparative analysis of the impact of e-cigarette vapor and cigarette smoke on human gingival fibroblasts

2020 ◽  
Vol 69 ◽  
pp. 105005
Author(s):  
M.F. Vermehren ◽  
N. Wiesmann ◽  
J. Deschner ◽  
J. Brieger ◽  
B. Al-Nawas ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Cigarette Smoke ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
The Impact

scholarly journals Comparison of visual, impedance spectroscopy and laser fluorescence methods in detecting early carious lesions on occlusal surfaces

2014 ◽  
Vol 27 (2) ◽  
pp. 102-104 ◽  
Author(s):  
Renata Chalas ◽  
Ilona Wojcik-Checinska ◽  
Joanna Zubrzycka-Wrobel ◽  
Dominika Piatek ◽  
Teresa Bachanek
Keyword(s):  
Impedance Spectroscopy ◽  
Quantitative Methods ◽  
Diagnostic Methods ◽  
Laser Fluorescence ◽  
Advanced Technology ◽  
Visual Examination ◽  
Dental Tissues ◽  
Carious Lesions ◽  
Diagnodent Pen ◽  
Occlusal Surfaces

Abstract The diagnostic management is a very important and integral part of the entire treatment process and has a direct influence on the decision-taking on the choice of the most appropriate form of therapy consistent with current knowledge. Knowledge of the morphology of hard dental tissues lesions has led to the development of quantitative methods for diagnosis and monitoring of dental caries, which enabled the implementation of appropriate treatments aimed at repairing than replacing damaged tissue. The aim of the study was to compare selected diagnostic methods: visual (ICDAS), impedance spectroscopy (CarieScan PRO) and laser fluorescence (Diagnodent Pen) in detecting caries in grooves on the chewing surfaces of molars and premolars. The obtained results indicated a high concordance of measurements performed with the Diagnodent Pen with the results of visual examination and a lower compliance of visual examination with the results obtained using the CarieScan PRO. A combination of visual and tactile method with tests using advanced technology provides greater opportunity to confirm the diagnosis of carious lesions requiring medical intervention.

scholarly journals The impact of implant abutment preparation on proliferation of human gingival fibroblasts

2020 ◽  
Vol 31 (S20) ◽  
pp. 167-167
Author(s):  
Dominyka Liaudanskaite ◽  
Rokas Borusevicius ◽  
Vygandas Rutkunas ◽  
Virginija Bukelskiene ◽  
Milda Alksne ◽  
...  
Keyword(s):  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Implant Abutment ◽  
The Impact

scholarly journals Ozone saline solution counteracts bisphosphonates noxious effects in primary human gingival fibroblasts

2019 ◽  
Author(s):  
Octavia Iulia Bălean ◽  
Alin Daniel Floare ◽  
Roxanne Focht ◽  
Daniela Jumanca ◽  
Dorina Elena Coricovac ◽  
...  
Keyword(s):  
Zoledronic Acid ◽  
Cell Viability ◽  
Saline Solution ◽  
Human Gingival Fibroblasts ◽  
Alamar Blue ◽  
Gingival Fibroblasts ◽  
Experimental Conditions ◽  
Antiresorptive Agents ◽  
The Impact ◽  
In Cells

Abstract Background Bisphosphonates are effective antiresorptive agents frequently used in the treatment of different bone disorders, as osteoporosis, Paget’s disease and tumours that cause osteolysis. A major concern related to bisphosphonates therapy is represented by osteonecrosis of jaw, a serious, debilitating, and mostly, a therapy-resistant disease, reported as a frequent side effect of bisphosphonates. In the present study were proposed two approaches: 1) to verify the impact of four commercially available bisphosphonates, very frequently used as oral (Fosamax - F and Actonel - A) and injectable (Ossica - O and Zoledronic acid - Z) therapy on primary human gingival fibroblasts - HGF viability and 2) to evaluate the protective effect of an ozone saline solution on HGF cells pretreated with bisphosphonates. Methods Alamar blue cell viability assay was performed to assess the effect of test compounds (1.5; 2.5; 5 and 10 μM) on gingival fibroblasts viability after a 24 h interval. An ozone - O3 saline solution – 80 µg/mL was added to bisphosphonates pretreated fibroblasts for 24 h and cell viability and cell morphology changes were determined by the means of Alamar blue test and microscopic images. Results Fosamax and Actonel induced a significant reduction of HGF cells viability even at concentrations as low as 2.5 μM (82 and 79.33%) and changes in cells morphology (round and floating cells), effects that were reversed by O3 saline solution administration: an increased cell viability after F and A at 2.5 μM: 147.54 and 120.11%), no changes in cells morphology and an improved confluence. Ossica and Zoledronic acid exerted no cytotoxic effect. Conclusions In conclusion, in these experimental conditions, injectable bisphosphonates (O and Z) proved to be safe for HGF cells, whereas oral compounds (F and A) were cytotoxic even at low concentrations, effects that were counteracted by O3 saline solution administration. Based on these data, ozone saline solution might represent a therapeutic alternative for bisphosphonates noxious effects on oral mucosa cells.

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