scholarly journals Significance Testing and Multivariate Analysis of Datasets from Surface Plasmon Resonance and Surface Acoustic Wave Biosensors: Prediction and Assay Validation for Surface Binding of Large Analytes

Sensors ◽  
2018 ◽  
Vol 18 (10) ◽  
pp. 3541 ◽  
Author(s):  
Mihaela Puiu ◽  
Lucian-Gabriel Zamfir ◽  
Valentin Buiculescu ◽  
Angela Baracu ◽  
Cristina Mitrea ◽  
...  

In this study, we performed uni- and multivariate data analysis on the extended binding curves of several affinity pairs: immobilized acetylcholinesterase (AChE)/bioconjugates of aflatoxin B1(AFB1) and immobilized anti-AFB1 monoclonal antibody/AFB1-protein carriers. The binding curves were recorded on three mass sensitive cells operating in batch configurations: one commercial surface plasmon resonance (SPR) sensor and two custom-made Love wave surface-acoustic wave (LW-SAW) sensors. We obtained 3D plots depicting the time-evolution of the sensor response as a function of analyte concentration using real-time SPR binding sensograms. These “calibration” surfaces exploited the transient periods of the extended kinetic curves, prior to equilibrium, creating a “fingerprint” for each analyte, in considerably shortened time frames compared to the conventional 2D calibration plots. The custom-made SAW sensors operating in different experimental conditions allowed the detection of AFB1-protein carrier in the nanomolar range. Subsequent statistical significance tests were performed on unpaired data sets to validate the custom-made LW-SAW sensors.

Sensors ◽  
2020 ◽  
Vol 20 (21) ◽  
pp. 6177
Author(s):  
Anastasios G. Samarentsis ◽  
Alexandros K. Pantazis ◽  
Achilleas Tsortos ◽  
Jean-Michel Friedt ◽  
Electra Gizeli

Surface plasmon resonance (SPR) and Love wave (LW) surface acoustic wave (SAW) sensors have been established as reliable biosensing technologies for label-free, real-time monitoring of biomolecular interactions. This work reports the development of a combined SPR/LW-SAW platform to facilitate simultaneous optical and acoustic measurements for the investigation of biomolecules binding on a single surface. The system’s output provides recordings of two acoustic parameters, phase and amplitude of a Love wave, synchronized with SPR readings. We present the design and manufacturing of a novel experimental set-up employing, in addition to the SPR/LW-SAW device, a 3D-printed plastic holder combined with a PDMS microfluidic cell so that the platform can be used in a flow-through mode. The system was evaluated in a systematic study of the optical and acoustic responses for different surface perturbations, i.e., rigid mass loading (Au deposition), pure viscous loading (glycerol and sucrose solutions) and protein adsorption (BSA). Our results provide the theoretical and experimental basis for future application of the combined system to other biochemical and biophysical studies.


Lab on a Chip ◽  
2016 ◽  
Vol 16 (7) ◽  
pp. 1224-1233 ◽  
Author(s):  
A. Sonato ◽  
M. Agostini ◽  
G. Ruffato ◽  
E. Gazzola ◽  
D. Liuni ◽  
...  

A novel, surface plasmon resonance phase-interrogation based microfluidic biosensor in which surface acoustic wave-driven mixing accelerates molecule binding kinetics up to 84% of the reaction time is presented.


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