scholarly journals Dielectrophoresis Prototypic Polystyrene Particle Synchronization toward Alive Keratinocyte Cells for Rapid Chronic Wound Healing

Sensors ◽  
2021 ◽  
Vol 21 (9) ◽  
pp. 3007
Author(s):  
Revathy Deivasigamani ◽  
Nur Nasyifa Mohd Maidin ◽  
M. F. Mohd Razip Wee ◽  
Mohd Ambri Mohamed ◽  
Muhamad Ramdzan Buyong

Diabetes patients are at risk of having chronic wounds, which would take months to years to resolve naturally. Chronic wounds can be countered using the electrical stimulation technique (EST) by dielectrophoresis (DEP), which is label-free, highly sensitive, and selective for particle trajectory. In this study, we focus on the validation of polystyrene particles of 3.2 and 4.8 μm to predict the behavior of keratinocytes to estimate their crossover frequency (fXO) using the DEP force (FDEP) for particle manipulation. MyDEP is a piece of java-based stand-alone software used to consider the dielectric particle response to AC electric fields and analyzes the electrical properties of biological cells. The prototypic 3.2 and 4.8 μm polystyrene particles have fXO values from MyDEP of 425.02 and 275.37 kHz, respectively. Fibroblast cells were also subjected to numerical analysis because the interaction of keratinocytes and fibroblast cells is essential for wound healing. Consequently, the predicted fXO from the MyDEP plot for keratinocyte and fibroblast cells are 510.53 and 28.10 MHz, respectively. The finite element method (FEM) is utilized to compute the electric field intensity and particle trajectory based on DEP and drag forces. Moreover, the particle trajectories are quantified in a high and low conductive medium. To justify the simulation, further DEP experiments are carried out by applying a non-uniform electric field to a mixture of different sizes of polystyrene particles and keratinocyte cells, and these results are well agreed. The alive keratinocyte cells exhibit NDEP force in a highly conductive medium from 100 kHz to 25 MHz. 2D/3D motion analysis software (DIPP-MotionV) can also perform image analysis of keratinocyte cells and evaluate the average speed, acceleration, and trajectory position. The resultant NDEP force can align the keratinocyte cells in the wound site upon suitable applied frequency. Thus, MyDEP estimates the Clausius–Mossotti factors (CMF), FEM computes the cell trajectory, and the experimental results of prototypic polystyrene particles are well correlated and provide an optimistic response towards keratinocyte cells for rapid wound healing applications.

Pressure has been used as the principal parameter in calculations of the fundamental vibrational frequencies of spherical drops of radius R , density ρ, and surface tension T carrying a charge Q or uncharged spheroidal drops of axial ratio a / b situated in a uniform electric field of strength E . Freely vibrating charged drops have a frequency f = f 0 ( 1 - Q 2 /16π R 3 T ) ½ , as shown previously by Rayleigh (1882) using energy considerations; f 0 is the vibrational frequency of non-electrified drops (Rayleigh 1879). The fundamental frequency of an uncharged drop in an electric field will decrease with increasing field strength and deformation a / b and will equal zero when E ( R )/ T ) ½ = 1.625 and a / b = 1.86; these critical values correspond to the disintegration conditions derived by Taylor (1964). An interferometric technique involving a laser confirmed the accuracy of the calculations concerned with charged drops. The vibration of water drops of radius around 2 mm was studied over a wide range of temperatures as they fell through electric fields either by suspending them in a vertical wind tunnel or allowing them to fall between a pair of vertical electrodes. Photographic analysis of the vibrations revealed good agreement between theory and experiment over the entire range of conditions studied even though the larger drops were not accurately spheroidal and the amplitude of the vibrations was large.


2018 ◽  
Vol 2018 ◽  
pp. 1-15
Author(s):  
Ying Li ◽  
Yu Gu ◽  
He Wang ◽  
Zhipeng Liu ◽  
Bing Song ◽  
...  

Galvanotaxis, or electrotaxis, plays an essential role in wound healing, embryogenesis, and nerve regeneration. Up until now great efforts have been made to identify the underlying mechanism related to galvanotaxis in various cells under direct current electric field (DCEF) in laboratory studies. However, abundant clinical research shows that non-DCEFs including monopolar or bipolar electric field may also contribute to wound healing and regeneration, although the mechanism remains elusive. Here, we designed a novel electric stimulator and applied DCEF, pulsed DCEF (pDCEF), and bipolar pulse electric field (bpEF) to the cells of Dictyostelium discoideum. The cells had better directional performance under asymmetric 90% duty cycle pDCEF and 80% duty cycle bpEF compared to DCEF, with 10 Hz frequency electric fields eliciting a better cell response than 5 Hz. Interestingly, electrically neutral 50% duty cycle bpEF triggered the highest migration speed, albeit in random directions. The results suggest that electric pulses are vital to galvanotaxis and non-DCEF is promising in both basic and clinical researches.


2010 ◽  
Vol 664 ◽  
pp. 286-296 ◽  
Author(s):  
DOV RHODES ◽  
EHUD YARIV

A dielectric drop is suspended within a dielectric liquid and is exposed to a uniform electric field. Due to polarization forces, the drop deforms from its initial spherical shape, becoming prolate in the field direction. At strong electric fields, the drop elongates significantly, becoming long and slender. For moderate ratios of the permittivities of the drop and surrounding liquid, the drop ends remain rounded. The slender limit was originally analysed by Sherwood (J. Phys. A, vol. 24, 1991, p. 4047) using a singularity representation of the electric field. Here, we revisit it using matched asymptotic expansions. The electric field within the drop is continued into a comparable solution in the ‘inner’ region, at the drop cross-sectional scale, which is itself matched into the singularity representation in the ‘outer’ region, at the drop longitudinal scale. The expansion parameter of the problem is the elongated drop slenderness. In contrast to familiar slender-body analysis, this parameter is not provided by the problem formulation, and must be found throughout the course of the solution. The drop aspect-ratio scaling, with the 6/7-power of the electric field, is identical to that found by Sherwood (J. Phys. A, vol. 24, 1991, p. 4047). The predicted drop shape is compared with the boundary-integral solutions of Sherwood (J. Fluid Mech., vol. 188, 1988, p. 133). While the agreement is better than that found by Sherwood (J. Phys. A, vol. 24, 1991, p. 4047), the weak logarithmic decay of the error terms still hinders an accurate calculation. We obtain the leading-order correction to the drop shape, improving the asymptotic approximation.


2020 ◽  
pp. 64-72
Author(s):  
Mustafa Erol ◽  
İldahan Özdeyiş Çolak

This work offers an unproblematic teaching tool for the instruction of challeng-ing concept of electric potential difference in a non-uniform electric field. Specifically, mathematical modelling process is employed and managed to comprehend and teach exceedingly difficult concepts of uniform and non-uniform electric fields, electrical potential difference, scalar products of vectors and also concept of path integral. In order to accomplish those tasks, initially a basic conducting panel/sheet, that is simply a wet cardboard, is designed as a part of the apparatus, together with a dc power supply, a multi meter and connecting cables. The established method is interesting in the sense that the 3D wet cardboard is novel, very practical and minimal costing, hence the approach offers physics educators fresh teaching routes and opportunities to clarify the puzzling concept of electrical potential difference and further.


2018 ◽  
Vol 844 ◽  
pp. 567-596 ◽  
Author(s):  
Yunqiao Liu ◽  
Dongdong He ◽  
Xiaobo Gong ◽  
Huaxiong Huang

In this paper, we investigate the dynamics of an encapsulated bubble in steady and oscillatory electric fields theoretically, based on a leaky dielectric model. On the bubble surface, an applied electric field generates a Maxwell stress, in addition to hydrodynamic traction and membrane mechanical stress. Our model also includes the effect of interfacial charge due to the jump of the current and the stretching of the interface. We focus on the axisymmetric deformation of the encapsulated bubble induced by the electric field and carry out our analysis using Legendre polynomials. In our first example, the encapsulating membrane is modelled as a nearly incompressible interface with bending rigidity. Under a steady uniform electric field, the encapsulated bubble resumes an elongated equilibrium shape, dominated by the second- and fourth-order shape modes. The deformed shape agrees well with experimental observations reported in the literature. Our model reveals that the interfacial charge distribution is determined by the magnitude of the shape modes, as well as the permittivity and conductivity of the external and internal fluids. The effects of the electric field on the natural frequency of the oscillating bubble are also shown. For our second example, we considered a bubble encapsulated with a hyperelastic membrane with bending rigidity, subject to an oscillatory electric field. We show that the bubble can modulate its oscillating frequency and reach a stable shape oscillation at an appreciable amplitude.


Author(s):  
Matthew R. Pearson ◽  
Jamal Seyed-Yagoobi

Past research in the area of pool boiling within the presence of electric fields has generally focused on the case of uniform field intensity. Any numerical or analytical studies of the effect of non-uniform fields on the motion of bubbles within a dielectric liquid medium have assumed that the bubbles will retain their spherical shape rather than deform. These studies also ignore changes to the electrical field caused by the presence of the bubbles. However, these assumptions are not necessarily accurate as, even in the case of a nominally uniform electric field distribution, bubbles can exhibit considerable physical deformation and the field can become noticeably affected in the vicinity of the bubble. This study explores the effect that a non-uniform electric field can have on vapor bubbles of a dielectric fluid by modeling the physical deformation of the bubble and the alteration of the surrounding field. Numerical results show that the imbalance of electrical stresses at the bubble surface exerts a net dielectrophoretic force on the bubble, propelling the bubble to the vicinity of weakest electric field, thereby enhancing the separation of liquid and vapor phases during pool boiling. However, the proximity of the bubble to one of the electrodes can considerably alter the bubble trajectory due to an attractive force that arises from local distortions of the potential and electric fields. This phenomenon cannot be predicted if bubble deformation and field distortion effects are neglected.


1970 ◽  
Vol 4 (3) ◽  
pp. 441-450 ◽  
Author(s):  
Barbara Abraham-Shrauner

Suppression of runaway of electrons in a weak, uniform electric field in a fully ionized Lorentz plasma by crossed magnetic and electric fields is analysed. A uniform, constant magnetic field parallel to a constant or harmonically time varying electric field does not alter runaway from that in the absence of the magnetic field. For crossed, constant fields the passage to runaway or to free motion as described by constant drift motion and spiral motion about the magnetic field is lengthened in time for strong magnetic fields. The new ‘runaway’ time scale is roughly the ratio of the cyclotron frequency to the collision frequency squared for cyclotron frequencies much greater than the collision frequency. All ‘runaway’ time scales may be given approximately by t2E Teff where tE is the characteristic time of the electric field and Teff is the ffective collision time as estimated from the appropriate component of the electrical conductivity.


Micromachines ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 423 ◽  
Author(s):  
Haoqing Zhang ◽  
Honglong Chang ◽  
Pavel Neuzil

Dielectric particles in a non-uniform electric field are subject to a force caused by a phenomenon called dielectrophoresis (DEP). DEP is a commonly used technique in microfluidics for particle or cell separation. In comparison with other separation methods, DEP has the unique advantage of being label-free, fast, and accurate. It has been widely applied in microfluidics for bio-molecular diagnostics and medical and polymer research. This review introduces the basic theory of DEP, its advantages compared with other separation methods, and its applications in recent years, in particular, focusing on the different electrode types integrated into microfluidic chips, fabrication techniques, and operation principles.


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