scholarly journals Optimization of Lactoperoxidase and Lactoferrin Separation on an Ion-Exchange Chromatography Step

Separations ◽  
2017 ◽  
Vol 4 (2) ◽  
pp. 10 ◽  
Author(s):  
Naeimeh Faraji ◽  
Yan Zhang ◽  
Ajay Ray
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Chromatography Step

Model-Based Process Challenge of an Industrial Ion-Exchange Chromatography Step

2011 ◽  
Vol 35 (1) ◽  
pp. 183-190 ◽  
Author(s):  
K. Westerberg ◽  
E. Broberg Hansen ◽  
M. Degerman ◽  
T. Budde Hansen ◽  
B. Nilsson
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Model Based ◽  
Chromatography Step

Using computer simulation to assist in the robustness analysis of an ion-exchange chromatography step

2005 ◽  
Vol 1063 (1-2) ◽  
pp. 99-109 ◽  
Author(s):  
Niklas Jakobsson ◽  
David Karlsson ◽  
Jan Peter Axelsson ◽  
Guido Zacchi ◽  
Bernt Nilsson
Keyword(s):  
Computer Simulation ◽  
Ion Exchange ◽  
Robustness Analysis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Chromatography Step

Model based robustness analysis of an ion-exchange chromatography step

2007 ◽  
Vol 1138 (1-2) ◽  
pp. 109-119 ◽  
Author(s):  
Niklas Jakobsson ◽  
Marcus Degerman ◽  
Emelie Stenborg ◽  
Bernt Nilsson
Keyword(s):  
Ion Exchange ◽  
Robustness Analysis ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Model Based ◽  
Chromatography Step

Simultaneous HPTLC identification of Fig, Senna and P. hybridus using Ion Exchange Chromatography

2017 ◽  
Author(s):  
B Nägele ◽  
J Wüthrich ◽  
S Toff ◽  
A Schenk
Keyword(s):  
Ion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography

Studies on an Inhibitor of Plasminogen Activation in Human Serum

1973 ◽  
Vol 30 (02) ◽  
pp. 414-424 ◽  
Author(s):  
Ulla Hedner
Keyword(s):  
Ion Exchange ◽  
Human Serum ◽  
Antithrombin Iii ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Specific Adsorption ◽  
Partial Purification ◽  
Plasminogen Activation ◽  
Preparative Electrophoresis

SummaryA procedure is described for partial purification of an inhibitor of the activation of plasminogen by urokinase and streptokinase. The method involves specific adsorption of contammants, ion-exchange chromatography on DEAE-Sephadex, gel filtration on Sephadex G-200 and preparative electrophoresis. The inhibitor fraction contained no antiplasmin, no plasminogen, no α1-antitrypsin, no antithrombin-III and was shown not to be α2 M or inter-α-inhibitor. It contained traces of prothrombin and cerulo-plasmin. An antiserum against the inhibitor fraction capable of neutralising the inhibitor in serum was raised in rabbits.

Analysis of mouse liver membrane proteins using multidimensional ion exchange chromatography and tandem mass spectrometry

2010 ◽  
Vol 28 (2) ◽  
pp. 115-122
Author(s):  
Zhuowei WANG ◽  
Fuli PENG ◽  
Yuan WANG ◽  
Wei TONG ◽  
Yan REN ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Membrane Proteins ◽  
Ion Exchange ◽  
Tandem Mass Spectrometry ◽  
Mouse Liver ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Tandem Mass ◽  
Liver Membrane

Review on the Application of Mixed-mode Chromatography for Separation of Structure Isoforms

2018 ◽  
Vol 20 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Tsutomu Arakawa
Keyword(s):  
Ion Exchange ◽  
Mixed Mode ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Charged State ◽  
Partial Structure ◽  
Reverse Phase ◽  
Oligomer Formation ◽  
Structure Rearrangement ◽  
Disulfide Scrambling

Proteins often generate structure isoforms naturally or artificially due to, for example, different glycosylation, disulfide scrambling, partial structure rearrangement, oligomer formation or chemical modification. The isoform formations are normally accompanied by alterations in charged state or hydrophobicity. Thus, isoforms can be fractionated by reverse-phase, hydrophobic interaction or ion exchange chromatography. We have applied mixed-mode chromatography for fractionation of isoforms for several model proteins and observed that cation exchange Capto MMC and anion exchange Capto adhere columns are effective in separating conformational isoforms and self-associated oligomers.

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