scholarly journals Biostimulation of Microbial Communities from Malaysian Agricultural Soil for Detoxification of Metanil Yellow Dye; a Response Surface Methodological Approach

2020 ◽  
Vol 13 (1) ◽  
pp. 138
Author(s):  
Fatin Natasha Amira Muliadi ◽  
Mohd Izuan Effendi Halmi ◽  
Samsuri Bin Abdul Wahid ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Microbial Communities ◽  
Response Surface ◽  
Ammonium Sulfate ◽  
Mixed Culture ◽  
Agricultural Soil ◽  
Bacterial Culture ◽  
Dye Decolorization ◽  
Mixed Bacterial Culture ◽  
Metanil Yellow

In the present study, a mixed culture from a local agricultural soil sample was isolated for Metanil Yellow (MY) dye decolorization. The metagenomic analysis confirmed that 42.6% has been dominated by genus Bacillus, while Acinetobacter (14.0%) is present in the microbial communities of the mixed culture. For fungi diversity analysis, around 97.0% was “unclassified” fungi and 3% was Candida. The preliminary investigation in minimal salt media (MSM) showed that 100% decolorization was achieved after 24 h of incubation. Response surface methodology (RSM) was successfully applied using Box-Behnken design (BBD) to study the effect of four independent parameters—MY dye concentration, glucose concentration, ammonium sulfate concentration, and pH—on MY dye decolorization by the mixed bacterial culture. The optimal conditions predicted by the desirability function were 73 mg/L of MY, 1.934% glucose, 0.433 g/L of ammonium sulfate, and a pH of 7.097, with 97.551% decolorization The correlation coefficients (R2 and R2 adj) of 0.913 and 0.825 indicate that the established model is suitable to predict the effectiveness of dye decolorization under the investigated condition. The MY decolorization of the mixed bacterial culture was not affected by the addition of heavy metals in the growth media. Among the 10 heavy metals tested, only copper gave 56.19% MY decolorization, whereas the others gave almost 100% decolorization. The decolorization potential of the mixed bacterial culture indicates that it could be effective for future bioremediation of soil-contaminated sites and treatment solutions of water bodies polluted with the MY dye.

scholarly journals Immobilization of Metanil Yellow Decolorizing Mixed Culture FN3 Using Gelling Gum as Matrix for Bioremediation Application

2020 ◽  
Vol 13 (1) ◽  
pp. 36
Author(s):  
Fatin Natasha Amira Muliadi ◽  
Mohd Izuan Effendi Halmi ◽  
Samsuri Bin Abdul Wahid ◽  
Siti Salwa Abd Gani ◽  
Khairil Mahmud ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Mixed Culture ◽  
Immobilized Cells ◽  
Desirability Function ◽  
Correlation Coefficients ◽  
Dye Decolorization ◽  
Gellan Gum ◽  
The Media ◽  
Metanil Yellow ◽  
Immobilized Beads

In this study, the Metanil Yellow (MY) decolorizing mixed culture, namely FN3, has been isolated from agriculture soil. The mixed culture was immobilized using gellan gum. In order to optimize the immobilization process for maximal dye decolorization, Response Surface Methodology (RSM) was performed. The optimal conditions for immobilization predicted by desirability function are 130 mg/L of MY dye concentration, 1.478% of gellan gum concentration, 50 beads and 0.6 cm of beads size with the percentage of decolorization of 90.378%. The correlation coefficients of the model (R2 and R2 adj) are 0.9767 and 0.9533, respectively. This indicates that the established model is suitable to predict the effectiveness of dye decolorization under the investigated condition. The immobilized beads of mixed culture FN3 were able to be reused up to 15 batches of decolorization. The immobilized cells also have high tolerance towards heavy metals. This was proven by higher dye decolorization rate by the immobilized cells even with the addition of heavy metals in the media. The decolorization potential of the mixed culture indicates that it could be useful for future bioremediation of soil contaminated sites and treatment solutions of water bodies polluted with MY dye.

scholarly journals Microbial Decolorization of Triazo Dye, Direct Blue 71: An Optimization Approach Using Response Surface Methodology (RSM) and Artificial Neural Network (ANN)

2020 ◽  
Vol 2020 ◽  
pp. 1-16
Author(s):  
Khairunnisa’ Mohd Zin ◽  
Mohd Izuan Effendi Halmi ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
A. Wahid Samsuri ◽  
...  
Keyword(s):  
Neural Network ◽  
Artificial Neural Network ◽  
Response Surface Methodology ◽  
Response Surface ◽  
Bacterial Culture ◽  
Dye Decolorization ◽  
Mixed Bacterial Culture ◽  
Direct Blue ◽  
Artificial Neural Network Ann ◽  
Direct Blue 71

The release of wastewater from textile dyeing industrial sectors is a huge concern with regard to pollution as the treatment of these waters is truly a challenging process. Hence, this study investigates the triazo bond Direct Blue 71 (DB71) dye decolorization and degradation dye by a mixed bacterial culture in the deficiency source of carbon and nitrogen. The metagenomics analysis found that the microbial community consists of a major bacterial group of Acinetobacter (30%), Comamonas (11%), Aeromonadaceae (10%), Pseudomonas (10%), Flavobacterium (8%), Porphyromonadaceae (6%), and Enterobacteriaceae (4%). The richest phylum includes Proteobacteria (78.61%), followed by Bacteroidetes (14.48%) and Firmicutes (3.08%). The decolorization process optimization was effectively done by using response surface methodology (RSM) and artificial neural network (ANN). The experimental variables of dye concentration, yeast extract, and pH show a significant effect on DB71 dye decolorization percentage. Over a comparative scale, the ANN model has higher prediction and accuracy in the fitness compared to the RSM model proven by approximated R2 and AAD values. The results acquired signify an efficient decolorization of DB71 dye by a mixed bacterial culture.

scholarly journals Isolation of Mixed Bacterial Culture from Rajshahi Silk Industrial Zone and their Efficiency in Azo Dye Decolorization

2015 ◽  
Vol 8 (10) ◽  
pp. 950 ◽  
Author(s):  
Farhana Parvin ◽  
Muhammed Mizanur Rahman ◽  
Md. Mahmudul Islam ◽  
Naoshin Jahan ◽  
Md. Pallob Ebna Shaekh ◽  
...  
Keyword(s):  
Azo Dye ◽  
Bacterial Culture ◽  
Dye Decolorization ◽  
Industrial Zone ◽  
Mixed Bacterial Culture ◽  
Azo Dye Decolorization

scholarly journals Hazards involved in the use of furazolidone for the prevention of salmonellosis in broiler chickens

1974 ◽  
Vol 72 (3) ◽  
pp. 349-354 ◽  
Author(s):  
Marjatta Rantala ◽  
E. Nurmi
Keyword(s):  
Mixed Culture ◽  
Broiler Chickens ◽  
Bacterial Culture ◽  
Intestinal Flora ◽  
Preventive Effect ◽  
Mixed Bacterial Culture ◽  
Inoculation Treatment

SUMMARYThe purpose of this work was to study the effects of interrupted, continuous and post-salmonella inoculation treatment with furazolidone in the feed on the colon-ization of Salmonella infantis in the intestines of chickens, as well as the influence of furazolidone in vitro on the effect of a mixed culture used for the prevention of salmonellosis in chickens.It was shown that chickens given interrupted treatment with 0·01% furazoli-done had significantly more salmonellas in the caeca than either chickens fed continuously with this drug or chickens without any treatment. The use of 0·01% furazolidone after inoculation with Salmonella infantis had no effect on Salmonella infantis in the caeca of chickens.The mixed bacterial culture from the normal intestinal flora lost its preventive effect on salmonellosis when cultured with 0·01% furazolidone.

scholarly journals Accumulation and bioconcentration of heavy metals in two phases from agricultural soil to plants in Usangu Agroecosystem-Tanzania

Heliyon ◽  
2021 ◽  
pp. e07514
Author(s):  
Marco Mng’ong’o ◽  
Linus K. Munishi ◽  
Patrick A. Ndakidemi ◽  
William Blake ◽  
Sean Comber ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Agricultural Soil ◽  
Two Phases

scholarly journals Nitrification exhibits Haldane kinetics in an agricultural soil treated with ammonium sulfate or dairy-waste compost

2010 ◽  
Vol 74 (2) ◽  
pp. 316-322 ◽  
Author(s):  
Teresa E. Koper ◽  
John M. Stark ◽  
Mussie Y. Habteselassie ◽  
Jeanette M. Norton
Keyword(s):  
Ammonium Sulfate ◽  
Agricultural Soil ◽  
Dairy Waste

scholarly journals Do soil bacterial communities respond differently to abrupt or gradual additions of copper?

2018 ◽  
Vol 95 (1) ◽  
Author(s):  
Michael McTee ◽  
Lorinda Bullington ◽  
Matthias C Rillig ◽  
Philip W Ramsey
Keyword(s):  
Heavy Metals ◽  
Soil Respiration ◽  
Microbial Communities ◽  
Bacterial Communities ◽  
Diversity Indices ◽  
Microbial Populations ◽  
Metal Concentrations ◽  
Soil Bacterial Communities ◽  
Bacterial Richness

ABSTRACTMany experiments that measure the response of microbial communities to heavy metals increase metal concentrations abruptly in the soil. However, it is unclear whether abrupt additions mimic the gradual and often long-term accumulation of these metals in the environment where microbial populations may adapt. In a greenhouse experiment that lasted 26 months, we tested whether bacterial communities and soil respiration differed between soils that received an abrupt or a gradual addition of copper or no copper at all. Bacterial richness and other diversity indices were consistently lower in the abrupt treatment compared to the ambient treatment that received no copper. The abrupt addition of copper yielded different initial bacterial communities than the gradual addition; however, these communities appeared to converge once copper concentrations were approximately equal. Soil respiration in the abrupt treatment was initially suppressed but recovered after four months. Afterwards, respiration in both the gradual and abrupt treatments wavered between being below or equal to the ambient treatment. Overall, our study indicates that gradual and abrupt additions of copper can yield similar bacterial communities and respiration, but these responses may drastically vary until copper concentrations are equal.

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