scholarly journals A Gold Growth-Based Plasmonic ELISA for the Sensitive Detection of Fumonisin B1 in Maize

Toxins ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 323 ◽  
Author(s):  
Shengnan Zhan ◽  
Lingyan Zheng ◽  
Yaofeng Zhou ◽  
Kesheng Wu ◽  
Hong Duan ◽  
...  

In this paper, a highly sensitive plasmonic enzyme-linked immunosorbent assay (pELISA) was developed for the naked-eye detection of fumonisin B1 (FB1). Glucose oxidase (GOx) was used as an alternative to horseradish peroxidase as the carrier of the competing antigen. GOx catalyzed the oxidation of glucose to produce hydrogen peroxide, which acted as a reducing agent to reduce Au3+ to Au on the surface of gold seeds (5 nm), This reaction led to a color change in the solution from colorless to purple, which was observable to the naked eye. Various parameters that could influence the detection performance of pELISA were investigated. The developed method exhibited a considerably high sensitivity for FB1 qualitative naked-eye detection, with a visible cut-off limit of 1.25 ng/mL. Moreover, the proposed pELISA showed a good linear range of 0.31–10 ng/mL with a half maximal inhibitory concentration (IC50) of 1.86 ng/mL, which was approximately 13-fold lower than that of a horseradish peroxidase- (HRP)-based conventional ELISA. Meanwhile, the proposed method was highly specific and accurate. In summary, the new pELISA exhibited acceptable accuracy and precision for sensitive naked-eye detection of FB1 in maize samples and can be applied for the detection of other chemical contaminants.

2019 ◽  
Vol 11 (29) ◽  
pp. 3706-3713 ◽  
Author(s):  
Mohammed Awad Abedalwafa ◽  
Yan Li ◽  
Chunfang Ni ◽  
Gang Yang ◽  
Lu Wang

Non-enzymatic colorimetric sensor strip for detection of metronidazole (MTZ) was designed and constructed, with high sensitivity and selectivity. Which can be used for naked-eye detection of MTZ with a visible color change from pink to purple.


RSC Advances ◽  
2015 ◽  
Vol 5 (105) ◽  
pp. 86463-86472 ◽  
Author(s):  
Ye Won Choi ◽  
Jae Jun Lee ◽  
Ga Rim You ◽  
Sun Young Lee ◽  
Cheal Kim

Colorimetric chemosensor was reported for detection of Cu2+ and F−via the color change from colorless to yellow and to orange.


2021 ◽  
Vol 21 (4) ◽  
pp. 2123-2131
Author(s):  
Alagan Jeevika ◽  
Dhesingh Ravi Shankaran

A simple, low-cost and highly selective nanosensor was developed for naked-eye detection of mercury ions (Hg2+) based on Eosin/silver nanocubes (Eosin/AgNCbs). Silver nanocubes (AgNCbs) were synthesized by polyol assisted chemical method. HR-TEM result shows the formed AgNCbs have a mean diameter of 84±0.005 nM (diagonally measured) and edge length of 55±0.01 nM. XRD result confirms that the AgNCbs are single crystalline in nature with a phase structure of face centered cubic (FCC) of silver. On interaction of Hg2+, AgNCbs exhibits a color change from gray to black up to 16.67 μM of Hg2+ owed to the formation of solid like bimetallic complex of Ag/Hg amalgam. The selectivity of AgNCbs was evaluated with several other toxic metal ions including, Mg2+, Ba2+, Ca4+, Pb2+, Cd4+, Zn2+, Co2+, Cu2+, K+ and Ni2+ and found good selectivity towards Hg2+. The sensitivity of the AgNCbs sensor system was tuned by using Eosin as a co-staining agent. The Eosin/AgNCbs showed a limit of detection of 60±0.050 nM with the color change from orange to purple. The results suggests that the Eosin/AgNCbs nanosensor exhibits good selectivity, sensitivity, repeatability and rapid response, which could be explored for real-time detection of Hg2+ in environmental and biological samples.


2021 ◽  
Vol 9 ◽  
Author(s):  
Zhifeng Deng ◽  
Cheng Wang ◽  
Haichang Zhang ◽  
Taotao Ai ◽  
Kaichang Kou

In recent years, the wide application of fluoride materials has grown rapidly, therefore excessive discharge in the surrounding environment, especially in drinking water and organic effluent, has become a potential hazard to humans, and has even resulted in fluorosis disease. The development of a highly effective and convenient method to recognize fluoride anions in surrounding environments seems necessary and urgent. Among which, the development of a colorimetric and fluorescence fluoride chemosensor with obvious color changing allowing for naked-eye detection with high sensitivity and selectivity is more interesting and challenging. In this minireview, current novel colorimetric and fluorescence chemosensors for fluoride anions by hydrogen-bond interaction are introduced, including obvious color changing by naked-eye detection, high sensitivity and selectivity, non-pollution and fluoride extraction ability, aqueous detection, and other additional functions. Finally, the perspective of the fluoride chemosensor design concept and potential evolution trends are pointed out.


2017 ◽  
Vol 30 (2) ◽  
pp. 225-230 ◽  
Author(s):  
Yan Zhou ◽  
Li-qiang Yan ◽  
Zhi-neng Kong ◽  
Wen-qi Du ◽  
Bao-ying Wu ◽  
...  

2019 ◽  
Vol 11 (26) ◽  
pp. 3280-3285 ◽  
Author(s):  
Zhen Huang ◽  
Cuiyan Wu ◽  
Yaqian Li ◽  
Zile Zhou ◽  
Ruihua Xie ◽  
...  

A turn-on fluorescent probe for cysteine with high selectivity was designed and synthetized. The probe can be used for “naked-eye” detection of Cys with an obvious color change from yellow to colorless. The probe could be applied for Cys detection in real human serum.


2016 ◽  
Vol 52 (13) ◽  
pp. 2748-2751 ◽  
Author(s):  
Xiang-Chun Li ◽  
Chun-Yu Wang ◽  
Yi Wan ◽  
Wen-Yong Lai ◽  
Li Zhao ◽  
...  

A T-shaped Schiff-base triazatruxene derivative (TATNFF) was designed, synthesized, and explored as a sensitive probe to detect HCl gas by the naked eye.


2020 ◽  
Vol 98 (10) ◽  
pp. 659-666
Author(s):  
Cheng Cao ◽  
Xingmei You ◽  
Lei Feng ◽  
Guanghong Luo ◽  
Guoren Yue ◽  
...  

Two new chromogenic sensors 1-(2-hydroxyphenyl)-3-(4-nitrophenyl)thiourea 1 and 1-(3-hydroxypyridin-2-yl)-3-(4-nitrophenyl)thiourea 2 bearing nitrophenyl and thiourea groups were designed and synthesized by one-step procedure and characterized through 1H NMR, 13C NMR, FTIR, and MS. The anion recognition property of the receptors was studied via naked-eye detection, UV–vis, and 1H NMR. Based on the existence of amino gen and hydroxyl moieties in receptors, receptors 1 and 2 exhibit obvious selectivity by the redshift of UV–vis signals, colour changes through naked-eye detection, and binding effects for F–, H2PO4–, and Ac–. Surprisingly, the detection limits of receptor 1 for F– and Ac– were calculated to be 5.45 × 10−7 and 2.11 × 10−7 (mol/L)−1, respectively, which indicated that F– and Ac– can be identified with high sensitivity by receptor 1. Besides, simple “test strips” were developed and were used as sensors for recognition of F–, H2PO4–, or Ac– in DMSO solution. Lastly, the mechanisms of the recognition process were studied through DFT calculation and 1H NMR titration.


Nanoscale ◽  
2016 ◽  
Vol 8 (8) ◽  
pp. 4458-4462 ◽  
Author(s):  
Lu Li ◽  
Yue Long ◽  
Jin-Ming Gao ◽  
Kai Song ◽  
Guoqiang Yang

Here we demonstrate a facile method for naked-eye detection of urea based on the structure color change of pH-sensitive photonic crystals.


Author(s):  
Hadiseh Yazdani Nyaki ◽  
Nosrat Mahmoodi ◽  
Meysam Pasandideh Nadamani

A novel tripod-chromogenic sensor contains central nucleus s-triazine and thiazolidine-2,4-dione ring (TCST) was designed, synthesized and applied as colorimetric probes in aqueous solutions of dimethyl sulfoxide (DMSO). The probe showed a highly sensitive and selective colorimetric sensor for naked-eye detection of Li+, with a different color change from colorless to yellow. The probe’s detection limit (LOD) toward Li+ was found to be 1.2 μM. The result of the Job’s plot showed 1:1 stoichiometry for the interaction between tripod-chemosensor and Li+ that this result was confirmed by 1H NMR titration experiments. The probe can also be used for biological activities depending on the results of microbial tests.


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