scholarly journals Detoxification of the Fumonisin Mycotoxins in Maize: An Enzymatic Approach

Toxins ◽  
2019 ◽  
Vol 11 (9) ◽  
pp. 523 ◽  
Author(s):  
Johanna Alberts ◽  
Gerd Schatzmayr ◽  
Wulf-Dieter Moll ◽  
Ibtisaam Davids ◽  
John Rheeder ◽  
...  

Enzymatic detoxification has become a promising approach for control of mycotoxins postharvest in grains through modification of chemical structures determining their toxicity. In the present study fumonisin esterase FumD (EC 3.1.1.87) (FUMzyme®; BIOMIN, Tulln, Austria), hydrolysing fumonisin (FB) mycotoxins by de-esterification, was utilised to develop an enzymatic reduction method in a maize kernel enzyme incubation mixture. Efficacy of the FumD FB reduction method in “low” and “high” FB contaminated home-grown maize was compared by monitoring FB1 hydrolysis to the hydrolysed FB1 (HFB1) product utilising a validated LC-MS/MS analytical method. The method was further evaluated in terms of enzyme activity and treatment duration by assessing enzyme kinetic parameters and the relative distribution of HFB1 between maize kernels and the residual aqueous environment. FumD treatments resulted in significant reduction (≥80%) in “low” (≥1000 U/L, p < 0.05) and “high” (100 U/L, p < 0.05; ≥1000 U/L, p < 0.0001) FB contaminated maize after 1 h respectively, with an approximate 1:1 µmol conversion ratio of FB1 into the formation of HFB1. Enzyme kinetic parameters indicated that, depending on the activity of FumD utilised, a significantly (p < 0.05) higher FB1 conversion rate was noticed in “high” FB contaminated maize. The FumD FB reduction method in maize could find application in commercial maize-based practices as well as in communities utilising home-grown maize as a main dietary staple and known to be exposed above the tolerable daily intake levels.

1969 ◽  
Vol 191 (1) ◽  
pp. 155-157 ◽  
Author(s):  
A.P. Brestkin ◽  
E.V. Rozengart ◽  
V.A. Samokish ◽  
I.N. Soboleva

1980 ◽  
Vol 185 (2) ◽  
pp. 535-536 ◽  
Author(s):  
A Cornish-Bowden ◽  
J T Wong

An observation by Duggleby [Biochem. J. (1979) 181, 255-256] that estimates of kinetic parameters by the jack-knife technique [Cornish-Bowden & Wong (1978) Biochem. J. 175, 969-976] are sometimes outside the range of estimates from which they are calculated has been examined. No significant correlation has been found between the occurrence of this behaviour and the actual quality of the estimates.


2006 ◽  
Vol 4 (3) ◽  
pp. 9
Author(s):  
F.A Leone ◽  
R.P.M. Furriel ◽  
I.A. Borin ◽  
J.C. McNamara

2015 ◽  
Vol 1 (2) ◽  
Author(s):  
Stanko Dimitrov ◽  
Gergana Velikova ◽  
Venko Beschkov ◽  
Svetoslav Markov

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