scholarly journals Ethylenediaminetetraacetic Acid Disodium Salt Acts as an Antifungal Candidate Molecule against Fusarium graminearum by Inhibiting DON Biosynthesis and Chitin Synthase Activity

Toxins ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 17
Author(s):  
Xiu-Shi Song ◽  
Kai-Xin Gu ◽  
Jing Gao ◽  
Jian-Xin Wang ◽  
Shao-Chen Ding ◽  
...  
Keyword(s):  
Ethylenediaminetetraacetic Acid ◽  
Fusarium Species ◽  
Field Tests ◽  
Disodium Salt ◽  
H2o2 Production ◽  
Chitin Synthesis ◽  
Head Blight ◽  
Control Effect ◽  
Ethylenediaminetetraacetic Acid Disodium Salt

Fusarium fungi are the cause of an array of devastating diseases affecting yield losses and accumulating mycotoxins. Fungicides can be exploited against Fusarium and deoxynivalenol (DON) production. However, Fusarium resistance to common chemicals has become a therapeutic challenge worldwide, which indicates that new control agents carrying different mechanisms of action are desperately needed. Here, we found that a nonantibiotic drug, ethylenediaminetetraacetic acid disodium salt (EDTANa2), exhibited various antifungal activities against Fusarium species and DON biosynthesis. The infection of wheat seeding caused by F. graminearum was suppressed over 90% at 4 mM EDTANa2. A similar control effect was observed in field tests. Mycotoxin production assays showed DON production was significantly inhibited, 47% lower than the control, by 0.4 mM EDTANa2. In vitro experiments revealed a timely inhibition of H2O2 production as quickly as 4 h after amending cultures with EDTANa2 and the expression of several TRI genes significantly decreased. Chitin synthases of Fusarium were Mn2+-containing enzymes that were strongly inhibited by Mn2+ deficiency. EDTANa2 inhibited chitin synthesis and destroyed the cell wall and cytomembrane integrity of Fusarium, mainly via the chelation of Mn2+ by EDTANa2, and thus led to Mn deficiency in Fusarium cells. Taken together, these findings uncover the potential of EDTANa2 as a fungicide candidate to manage Fusarium head blight (FHB) and DON in agricultural production.

Synthesis of Ethylenediaminetetraacetic Acid Disodium Salt Functionalized Magnetite/Chitosan Nanospheres for the Highly Efficient Removal of Methylene Blue

2021 ◽  
Vol 21 (3) ◽  
pp. 1694-1702
Author(s):  
Mengke Zheng ◽  
Zhenguo Wu ◽  
Yang Wang ◽  
Nannan Chen ◽  
Zhaogang Teng ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Ethylenediaminetetraacetic Acid ◽  
Disodium Salt ◽  
Pseudo Second Order ◽  
Sorption Parameters ◽  
Adsorption Kinetics And Isotherm ◽  
Adsorption Desorption ◽  
The Impact ◽  
Desorption Cycle ◽  
Ethylenediaminetetraacetic Acid Disodium Salt

In this paper, novel Ethylenediaminetetraacetic acid disodium salt (EDTA) functionalized magnetite/ chitosan nanospheres (Fe3O4/CS-EDTA) are synthesized by combining solvothermal method and chemical modification, and they are further applied as a kind of adsorbent to eliminate dye of methylene blue (MB) from wastewater. The properties as well as structure exhibited by the fabricated adsorbent are characterized through FTIR, XRD, TG and TEM, together with VSM. The impact exerted by sorption parameters (time of contact, initial dye concentration, temperature, etc.) on the adsorptions were evaluated in batch system. These results demonstrated that our magnetic materials held the adsorption capacity for MB of 256 mg g−1 (pH = 11), and the kinetic model of pseudo-second-order and the Langmuir model could make an effective simulation regarding the adsorption kinetics and isotherm, respectively. Besides, the external magnetic field can assist in easily separating dye adsorbed Fe3O4/CS-EDTA from solution for regeneration. The removal efficiency of recycled adsorbents remained above 92% in the 5th adsorption/desorption cycle. These superioritiesmake Fe3O4/CS-EDTA a high-efficientmultifunctional adsorbent for removing dyes from wastewater.

Characterization of an antifungal soil bacterium and its antagonistic activities againstFusariumspecies

2003 ◽  
Vol 49 (4) ◽  
pp. 253-262 ◽  
Author(s):  
Yiu-Kwok Chan ◽  
Wayne A McCormick ◽  
Keith A Seifert
Keyword(s):  
Bacillus Subtilis ◽  
Culture Filtrate ◽  
Fusarium Species ◽  
Hyphal Growth ◽  
Antagonistic Activity ◽  
Plant Diseases ◽  
Ear Rot ◽  
In Planta ◽  
Head Blight

Bacteria were isolated from a cultivated soil and screened for antagonistic activity against Fusarium graminearum, a predominant agent of ear rot and head blight in cereal crops. Based on its in vitro effectiveness, isolate D1/2 was selected for characterization and identified as a strain of Bacillus subtilis by phenotypic tests and comparative analysis of its 16S ribosomal RNA gene (rDNA) sequence. It inhibited the mycelial growth of a collection of common fungal phytopathogens, including eight Fusarium species, three other ascomycetes, and one basidiomycete. The cell-free culture filtrate of D1/2 at different dilutions was active against macroconidium germination and hyphal growth of F. graminearum, depending on the initial macroconidium density. It induced the formation of swollen hyphal cells in liquid cultures of this fungus grown from macroconidia. A bioassay also demonstrated that D1/2 offered in planta protection against the damping-off disease in alfalfa seedlings caused by F. graminearum, while the type strain of B. subtilis was ineffective. Hence, B. subtilis D1/2 or its culture filtrate has potential application in controlling plant diseases caused by Fusarium.Key words: antifungal activity, Bacillus subtilis, biological control, biopesticide, Fusarium species.

In vitro evaluation of antimicrobial peptides against Fusarium species

2009 ◽  
Vol 89 (4) ◽  
pp. 739-743 ◽  
Author(s):  
A Badea ◽  
F Eudes ◽  
R Graf ◽  
A Laroche ◽  
A E Berg ◽  
...  
Keyword(s):  
Antimicrobial Peptides ◽  
Fusarium Head Blight ◽  
Key Words ◽  
Mycelial Growth ◽  
Fusarium Species ◽  
Common Species ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Head Blight

The in vitro activity of five antimicrobial peptides was evaluated against several Fusarium species that affect wheat in Canada. Among the peptides tested (10R, 11R, BMAP-18, MsrA2 and MsrA3) 10R was most effective against conidial germination, whereas MsrA2 showed activity against mycelial growth. Antimicrobial peptides 10R and MsrA2 were identified as potential candidates for engineering resistance against common species causing fusarium head blight in wheat. Key words: Antimicrobial peptides, fusarium head blight, conidia, mycelia

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