Heat shock protein 70 as an indicator of toxic heavy metal exposure and oxidative stress in the serum of asymptomatic shipyard welders

The present study investigated the effects of ferulic acid (FA) on pentylenetetrazole (PTZ)-induced seizures, oxidative stress markers (malondialdehyde (MDA), catalase, and reduced glutathione (GSH)), connexin (Cx) 43, heat shock protein 70 (Hsp 70), and monoamines (serotonin (5-HT) and norepinephrine (NE)) levels in a rat model of PTZ-induced kindling. Sixty Sprague Dawley rats were divided into 5 equal groups: (a) normal group; (b) FA group: normal rats received FA at a dose of 40 mg/kg daily; (c) PTZ group: normal rats received PTZ at a dose of 50 mg/kg i.p. on alternate days for 15 days; (d) FA-before group: treatment was the same as for the PTZ group, except rats received FA; and (e) FA-after group: rats received FA from sixth dose of PTZ. PTZ caused a significant increase in MDA, Cx43, and Hsp70 along with a significant decrease in GSH, 5-HT, and NE levels and CAT activity in the hippocampus (p < 0.05). Pre- and post-treatment with FA caused significant improvement in behavioral parameters, MDA, CAT, GSH, 5-HT, NE, Cx43 expression, and Hsp70 expression in the hippocampal region (p < 0.05). We conclude that FA has neuroprotective effects in PTZ-induced epilepsy, which might be due to attenuation of oxidative stress and Cx43 expression and upregulation of neuroprotective Hsp70 and neurotransmitters (5-HT and NE).

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Effect of Probiotic and Amla Powder Supplementation on Metabolic Profile and Milk Production of Summer Stressed Buffaloes Residing in Heavy Metal Polluted Areas of Ludhiana

Indian Journal of Animal Research ◽

10.18805/ijar.b-4270 ◽

2021 ◽

Author(s):

Pranesh V. Yeotikar ◽

Shashi Nayyar ◽

Chanchal Singh ◽

C.S. Mukhopadhyay ◽

Sandeep Sodhi Kakkar ◽

...

Keyword(s):

Oxidative Stress ◽

Heavy Metal ◽

Milk Production ◽

Metabolic Profile ◽

Antioxidant Status ◽

Live Cells ◽

Metal Exposure ◽

Heavy Metal Exposure ◽

Exposed Area ◽

Oral Supplementation

Background: Oxidative stress is a unified concept for the assessment of metabolic status of buffaloes affected by a cocktail of heavy metal exposure from environment and it may significantly affect the metabolic profile and milk production during summer season. The present study aimed at evaluation of effect of probiotic (Saccharomyces cerevisiae strain 1026) and amla powder (Embilica officinalis) supplementation on levels of heavy metals, antioxidant status, oxidative stress in lymphocytes, metabolic profile and milk production of summer stressed buffaloes exposed to environmental heavy metal pollution. Methods: Twenty summer stressed lactating Murrah buffaloes of the same age group, close parity and in early lactation from the heavy metal exposed area were divided into two groups: Control (without supplementation) and Treatment group (supplemented with Saccharomyces cerevisiae1026) @ 50 billion live cells / animal /day and Amla powder @ 86 mg/kg body weight for 30 days. Blood and milk samples were collected from both the groups on the day of starting of supplementation (i.e. day 0) and on there after days 15 and 30 and analyzed for antioxidant status, metabolic profile, milk yield and quality.Result: Oral supplementation of probiotic and amla powder for 30 days improved the antioxidant status, metabolic profile and milk production of summer stressed buffaloes residing in heavy metal exposed area. The percentage monetary gains from buffaloes fed probiotic and amla powder was 13.68% more as compared to control.

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