scholarly journals Prospects for the Use of Combined Lysozyme Drugs in COVID-19

2021 ◽  
Vol 17 (24) ◽  
pp. 12-17
Author(s):  
A.V. Gorelov ◽  
◽  
D.V. Usenko ◽  
L.P. Antonova ◽  
L.I. Kozlovskaya ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Cetylpyridinium Chloride ◽  
In Vitro Study ◽  
Antiviral Effect ◽  
Local Immunity ◽  
Culture Studies ◽  
Gradual Accumulation ◽  
Coronavirus Infection ◽  
The Moment

According to statistics, 81% of cases of COVID-19 coronavirus infection occur in a mild or moderate form. These patients make up the main part of the flow of calls to a general practitioner at the moment. The difficulties that therapists face during a pandemic are associated with finding the optimal therapy for the disease due to the gradual accumulation of knowledge about a new RNA-containing virus. The article presents the data of a study of the antiviral activity of the drug Lysobact Complete® in vitro on a Vero cell culture. Studies of previous years confirm the antiviral, anti-inflammatory, strengthening local immunity effect of lysozyme hydrochloride, the antiviral effect of cetylpyridinium chloride, both are the components of the drug Lysobact Complete® dosed spray for topical use. This in vitro study confirmed the antiviral activity of the drug on the SARS-CoV-2 virus, which makes its use promising in complex therapy of patients with COVID-19. The theoretical justification of the practical use of the drug is given. The authors hope that the research data will be useful to doctors when providing medical care to patients with COVID-19

scholarly journals EGYVIR: An immunomodulatory herbal extract with potent antiviral activity against SARS-CoV-2

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241739
Author(s):  
Wael H. Roshdy ◽  
Helmy A. Rashed ◽  
Ahmed Kandeil ◽  
Ahmed Mostafa ◽  
Yassmin Moatasim ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Nuclear Translocation ◽  
Antiviral Agents ◽  
In Vitro Study ◽  
Antiviral Effect ◽  
Black Pepper ◽  
Infection Process ◽  
Herbal Extract

Due to the challenges for developing vaccines in devastating pandemic situations of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), developing and screening of novel antiviral agents are peremptorily demanded. Herein, we developed EGYVIR as a potent immunomodulatory herbal extract with promising antiviral activity against SARS-CoV-2. It constitutes of a combination of black pepper extract with curcumin extract. The antiviral effect of EGYVIR extract is attributed to the two key phases of the disease in severe cases. First, the inhibition of the nuclear translocation of NF-kβ p50, attenuating the SARS-CoV-2 infection-associated cytokine storm. Additionally, the EGYVIR extract has an in vitro virucidal effect for SARS-CoV-2. The in vitro study of EGYVIR extract against SARS-CoV-2 on Huh-7 cell lines, revealed the potential role of NF-kβ/TNFα/IL-6 during the infection process. EGYVIR antagonizes the NF-kβ pathway in-silico and in-vitro studies. Consequently, it has the potential to hinder the release of IL-6 and TNFα, decreasing the production of essential cytokines storm elements.

scholarly journals In vitro antiviral activity of phlorotannins isolated from Ecklonia cava against porcine epidemic diarrhea coronavirus infection and hemagglutination

2013 ◽  
Vol 21 (15) ◽  
pp. 4706-4713 ◽  
Author(s):  
Hyung-Jun Kwon ◽  
Young Bae Ryu ◽  
Young-Min Kim ◽  
Naaleum Song ◽  
Cha Young Kim ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Ecklonia Cava ◽  
Coronavirus Infection ◽  
Porcine Epidemic Diarrhea

Destructive effect of RNаse a on the SARS-CoV-2 virus (in vitro research)

2021 ◽  
Vol 21 (3) ◽  
pp. 131-134
Author(s):  
Ilya A. Morozov ◽  
Anatoly P. Godovalov ◽  
Denis A. Oborin
Keyword(s):  
Antiviral Activity ◽  
In Vitro Study ◽  
Primary Diagnosis ◽  
Ribonuclease A ◽  
Medical Community ◽  
Destructive Effect ◽  
Environmental Objects ◽  
Tick Borne Encephalitis ◽  
Prevention And Treatment

BACKGROUND: For today, the most important and discussed issue in the professional medical community is the problem of prevention and treatment of a new coronaviral infection (COVID-19). The main reason for the non-decreasing increase in morbidity and mortality is the absence of an etiotropic drug. In our study, it is proposed to use a previously available drug for the treatment of tick-borne encephalitis, ribonuclease A, obtained from the pancreas of cattle. AIM: The aim of investigation was to study the antiviral activity of RNаse A against SARS-CoV-2 in in vitro experiments. MATERIALS AND METHODS: The experiment used samples of 50 patients with a confirmed (by PCR) primary diagnosis of a new coronoviral infection COVID-19. The preparation for the study was served by ribonuclease A (neoFroxx GmbH, Germany) at a concentration of 0.5; 1; 5 and 10 mg/ml, incubated at 4 and 37C, the exposure was 20 minutes, 20 hours. A set of reagents OTT-PCR-RV-SARS-CoV-2 (Syntol, Russia) was used as test systems. RESULTS: of the current study is the revealed antiviral activity of ribonuclease A at a minmal concentration of 0.5 mg/ml during incubation from 20 minutes to 20 hours, in the temperature range of 437C. CONCLUSIONS: The data obtained in the in vitro study confirmed the ability of ribonuclease A to destroy viral RNA, which suggests the possible use of the drug both for the treatment of patients and for the treatment of environmental objects.

scholarly journals Determination of the maximum inhibitory dilution of cetylpyridinium chloride-based mouthwashes against staphylococcus aureus: an in vitro study

2008 ◽  
Vol 16 (4) ◽  
pp. 275-279 ◽  
Author(s):  
Evandro Watanabe ◽  
Juliane Maria Guerreiro Tanomaru ◽  
Andresa Piacezzi Nascimento ◽  
Fumio Matoba-Júnior ◽  
Mario Tanomaru-Filho ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Cetylpyridinium Chloride ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals The in vitro and in vivo potency of CT-P59 against Delta and its associated variants of SARS-CoV-2

2021 ◽  
Author(s):  
Dong-Kyun Ryu ◽  
Hye-Min Woo ◽  
Bobin Kang ◽  
Hanmi Noh ◽  
Jong-In Kim ◽  
...  
Keyword(s):  
Antiviral Activity ◽  
Respiratory Tract ◽  
Animal Studies ◽  
Antiviral Effect ◽  
The World ◽  
Symptom Remission ◽  
Challenge Experiment ◽  
Reduced Activity

The Delta variant originally from India is rapidly spreading across the world and causes to resurge infections of SARS-CoV-2. We previously reported that CT-P59 presented its in vivo potency against Beta and Gamma variants, despite its reduced activity in cell experiments. Yet, it remains uncertain to exert the antiviral effect of CT-P59 on the Delta and its associated variants (L452R). To tackle this question, we carried out cell tests and animal study. CT-P59 showed reduced antiviral activity but enabled neutralization against Delta, Epsilon, and Kappa variants in cells. In line with in vitro results, the mouse challenge experiment with the Delta variant substantiated in vivo potency of CT-P59 showing symptom remission and virus abrogation in the respiratory tract. Collectively, cell and animal studies showed that CT-P59 is effective against the Delta variant infection, hinting that CT-P59 has therapeutic potency for patients infected with Delta and its associated variants.

Antiviral Activity of Dicrocephala Integrifolia (Kuntze) Against Herpes Simplex Type-1 Virus: An in vitro Study

2018 ◽  
Vol 8 (2) ◽  
pp. 81-85 ◽  
Author(s):  
Abdi Hussein Hadun ◽  
James Mucunu Mbaria ◽  
Gabriel Oluga Aboge ◽  
Mitchel Otieno Okumu ◽  
Antony Letoyah Yiaile
Keyword(s):  
Herpes Simplex ◽  
Antiviral Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Herpes Simplex Type

Cytotoxicity, Antibiotic Combination and Antiviral Activity of Papain Enzyme: In vitro study

2020 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Sara H. Mohamed ◽  
Walaa S. Mohamed ◽  
Mohamed N. F. Shaheen ◽  
Elmahdy M. Elmahdy ◽  
Mona I. Mabrouk
Keyword(s):  
Antiviral Activity ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antibiotic Combination

scholarly journals EPSTI1 Is Involved in IL-28A-Mediated Inhibition of HCV Infection

2015 ◽  
Vol 2015 ◽  
pp. 1-13 ◽  
Author(s):  
Xianghe Meng ◽  
Darong Yang ◽  
Rong Yu ◽  
Haizhen Zhu
Keyword(s):  
Life Cycle ◽  
Antiviral Activity ◽  
Viral Replication ◽  
Virus Replication ◽  
Antiviral Effect ◽  
Vital Role ◽  
Hcv Infection ◽  
Rnase L ◽  
Interferon Treatment

It has been reported that IFN-λs inhibit HCV replication in vitro. But the mechanisms of how IL-28A conducts antiviral activity and the functions of IL-28A-induced ISGs (IFN-stimulated genes) are not fully understood. In this study, we found that IL-28A has the antiviral effect on HCV life cycle including viral replication, assembly, and release. IL-28A and IFN-αsynergistically inhibit virus replication. EPSTI1 (epithelial-stromal interaction 1), one of IL-28A-induced ISGs, plays a vital role in IL-28A-mediated antiviral activity. Furthermore, forced expression of EPSTI1 effectively inhibits HCV replication in the absence of interferon treatment, and knockdown of EPSTI1 contributes to viral enhancement. EPSTI1 can activate PKR promoter and induce several PKR-dependent genes, including IFN-β, IFIT1, OAS1, and RNase L, which is responsible for EPSTI1-mediated antiviral activity.

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