scholarly journals Determination of antibiotic susceptibility, ESBL genes and pulsed-field gel electrophoresis profiles of extended-spectrum β-lactamase-containing Escherichia coli isolates

2019 ◽  
Author(s):  
Hidayet Tutun ◽  
Alper Karagöz ◽  
Levent Altıntaş ◽  
Nadir Koçak
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Antibiotic Susceptibility ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Extended Spectrum

scholarly journals Emergence of Enterobacteriaceae Isolates Producing CTX-M Extended-Spectrum β-Lactamase in Austria

2006 ◽  
Vol 50 (2) ◽  
pp. 785-787 ◽  
Author(s):  
Alexandra Eisner ◽  
Elizabeth J. Fagan ◽  
Gebhard Feierl ◽  
Harald H. Kessler ◽  
Egon Marth ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
United Kingdom ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
E Coli ◽  
The United Kingdom ◽  
Extended Spectrum ◽  
Epidemiological Link ◽  
Esbl Producers

ABSTRACT Among 149 extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae isolates collected from patients in southeast Austria from 1998 to 2004, 38 Escherichia coli isolates and 11 Klebsiella spp. were CTX-M producers. The proportion of CTX-M-producers among all ESBL producers rose from 0% in 1998 to 58% in 2004. In general, CTX-M-producers had heterogeneous pulsed-field gel electrophoresis patterns, but one E. coli isolate was identical to a United Kingdom epidemic CTX-M-15-producing strain, although no epidemiological link with the United Kingdom was apparent.

scholarly journals Clinical Isolates of Enterobacteriaceae Producing Extended-Spectrum β-Lactamases: Prevalence of CTX-M-3 at a Hospital in China

2003 ◽  
Vol 47 (2) ◽  
pp. 790-793 ◽  
Author(s):  
Hui Wang ◽  
Swathi Kelkar ◽  
Weiyuan Wu ◽  
Minjun Chen ◽  
John P. Quinn
Keyword(s):  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Teaching Hospital ◽  
Gel Electrophoresis ◽  
Enterobacter Cloacae ◽  
Pulsed Field Gel Electrophoresis ◽  
Clinical Isolates ◽  
Citrobacter Freundii ◽  
Pulsed Field ◽  
Extended Spectrum

ABSTRACT The prevalence of extended-spectrum β-lactamase-producing strains was demonstrated in 5 of 44 (11.4%) Escherichia coli, 17 of 43 (39.5%) Klebsiella pneumoniae, 3 of 50 (6.0%) Enterobacter cloacae, and 2 of 25 (8.0%) Citrobacter freundii strains at a teaching hospital in China. Nineteen of these 27 strains expressed CTX-M-3 β-lactamase (pI 8.6). A subset of the clinical isolates expressing the CTX-M-3 enzyme, tested by pulsed-field gel electrophoresis, revealed multiple clones. Five isolates expressed a novel enzyme, SHV-43 (pI 8.0), which had two substitutions (Leu113Phe and Thr149Ser) compared with SHV-1.

scholarly journals Monitoring and Characterization of Extended-Spectrum β-Lactamases in Escherichia coli Strains from Healthy and Sick Animals in Spain in 2003

2005 ◽  
Vol 49 (3) ◽  
pp. 1262-1264 ◽  
Author(s):  
Laura Briñas ◽  
Miguel Angel Moreno ◽  
Tirushet Teshager ◽  
Yolanda Sáenz ◽  
María Concepción Porrero ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Extended Spectrum ◽  
Genes Encoding ◽  
M 14

ABSTRACT Genes encoding CTX-M-14, CTX-M-9, CTX-M-1, CTX-M-32, SHV-12, TEM-52, or CMY-2 β-lactamases were detected in 21 Escherichia coli strains recovered during 2003 from sick animals (11 of 459 [2.4%] strains) and healthy animals (10 of 158 [6.3%] strains) in Spain. Twelve of these strains harbored bla CTX-M genes and showed unrelated pulsed-field gel electrophoresis patterns.

Occurrence of extended-spectrum β-lactamase, AmpC and virulence genes in Escherichia coli isolates from vegetable salads in Morocco

2017 ◽  
Vol 119 (7) ◽  
pp. 1633-1647
Author(s):  
Kaotar Nayme ◽  
Abouddihaj Barguigua ◽  
Brahim Bouchrif ◽  
Idrissa Diawara ◽  
Fatima El Otmani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Virulence Genes ◽  
Phylogenetic Group ◽  
Pulsed Field Gel Electrophoresis ◽  
Resistant Bacteria ◽  
Content Type ◽  
Pulsed Field ◽  
E Coli ◽  
Extended Spectrum

Purpose The purpose of this paper is to assess the occurrence of the extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamase genes in 144 Escherichia coli isolates recovered from 160 vegetable salad samples. Design/methodology/approach Among the 144 E. coli isolates recovered from 160 vegetable salads, 17 (12 percent) ceftazidime-resistant isolates were screened for ESBL production with the double disk-diffusion test. The ESBL-producing isolates were characterized for antimicrobial resistance, the presence of virulence genes and plasmid-mediated quinolone resistance (PMQR) determinants. The isolates were also subjected to phylogenetic group typing. The existence of plasmid AmpC genes and mutations in the regulatory region of the chromosomal AmpC gene was assessed using polymerase chain reaction (PCR) and sequencing. All β-lactamase isolates were further characterized by pulsed-field gel electrophoresis to determine the genetic relatedness. Findings Overall, 17 (12 percent) of the 144 E. coli isolates studied were ceftazidime resistant. Among the 17 isolates, 13 (77 percent) were multidrug resistant and four (23.5 percent) were ESBL producers. The bla CTX-M14 was the only gene detected. Of the 12 AmpC-producing isolates, three (18 percent) harbored plasmid-encoded AmpC and sequencing analysis of the chromosomal AmpC genes revealed mutations in the promoter/attenuator region. PMQR determinants were detected in 9 (52 percent) isolates. A was the most prevalent phylogenetic group (56 percent), followed by groups B1 (31 percent), D (6 percent), and B2 (6 percent). PCR showed that six (50 percent) ESBL/AmpC-producing E. coli isolates carried one and/or two virulence genes. Pulsed-field gel electrophoresis showed no epidemiological relationship between these isolates. Originality/value This study places vegetable salads within the spectrum of ecological niches that may be vehicles for antibiotic-resistant bacteria/genes with clinical interest and these findings are worthy of attention as their spread to humans by ingestion cannot be dismissed.

scholarly journals Carriage of Escherichia coli Producing CTX-M-Type Extended-Spectrum β-Lactamase in Healthy Vietnamese Individuals

2015 ◽  
Vol 59 (10) ◽  
pp. 6611-6614 ◽  
Author(s):  
Thi Mai Huong Bui ◽  
Itaru Hirai ◽  
Shuhei Ueda ◽  
Thi Kim Ngan Bui ◽  
Kouta Hamamoto ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Fecal Samples ◽  
Content Type ◽  
Pulsed Field ◽  
E Coli ◽  
Extended Spectrum

ABSTRACTHealthy carriage of CTX-M-type extended-spectrum β-lactamase (ESBL)-producingEscherichia coliwas examined by thrice collecting fecal samples from the same 199 healthy Vietnamese subjects every 6 months. Using pulsed-field gel electrophoresis (PFGE), identical PFGE patterns throughout the three samplings were not observed, although prevalence ofE. coliin the subjects was around 50% in the three samplings. Our results suggested a short carriage period of the CTX-M-type ESBL-producingE. coliin healthy Vietnamese subjects.

scholarly journals Increased Mutation Frequencies in Escherichia coli Isolates Harboring Extended-Spectrum β-Lactamases

2005 ◽  
Vol 49 (11) ◽  
pp. 4754-4756 ◽  
Author(s):  
María-Rosario Baquero ◽  
Juan Carlos Galán ◽  
María del Carmen Turrientes ◽  
Rafael Cantón ◽  
Teresa M. Coque ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Gel Electrophoresis ◽  
Mutation Frequency ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
E Coli ◽  
Extended Spectrum

ABSTRACT Hypermutable (mutation frequency [f], ≥4 × 10−8) Escherichia coli strains were more frequently found (43%) in a collection of 89 extended-spectrum β-lactamase (ESBL)-producing isolates from different patients (77 pulsed-field gel electrophoresis clones, 12 ESBL types) than in non-ESBL E. coli (26%) strains (P = 0.03). Among urinary tract isolates, the frequency of hypermutation was 40% in ESBL versus 26% in non-ESBL isolates (P = 0.03).

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