Urine collection bags.

The potential of first-void (FV) urine as a non-invasive liquid biopsy for detection of human papillomavirus (HPV) DNA and other biomarkers has been increasingly recognized over the past decade. In this study, we investigated whether the volume of this initial urine stream has an impact on the analytical performance of biomarkers. In parallel, we evaluated different DNA extraction protocols and introduced an internal control in the urine preservative. Twenty-five women, diagnosed with high-risk HPV, provided three home-collected FV urine samples using three FV urine collection devices (Colli-Pee) with collector tubes that differ in volume (4, 10, 20 mL). Each collector tube was prefilled with Urine Conservation Medium spiked with phocine herpesvirus 1 (PhHV-1) DNA as internal control. Five different DNA extraction protocols were compared, followed by PCR for GAPDH and PhHV-1 (qPCR), HPV DNA, and HBB (HPV-Risk Assay), and ACTB (methylation-specific qPCR). Results showed limited effects of collection volume on human and HPV DNA endpoints. In contrast, significant variations in yield for human endpoints were observed for different DNA extraction methods (p < 0.05). Additionally, the potential of PhHV-1 as internal control to monitor FV urine collection, storage, and processing was demonstrated.

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Development and double cross-validation of new spot urine sodium equation to predict 24-h urine sodium in the Malaysian population

Journal of Health Population and Nutrition ◽

10.1186/s41043-021-00232-3 ◽

2021 ◽

Vol 40 (S1) ◽

Author(s):

Fatimah Othman ◽

Rashidah Ambak ◽

Mohd Azahadi Omar ◽

Suzana Shahar ◽

Noor Safiza Mohd Nor ◽

...

Keyword(s):

Cross Validation ◽

Sodium Intake ◽

Study Groups ◽

Urine Collection ◽

Urine Sodium ◽

Data Set ◽

Spot Urine ◽

Malaysian Population ◽

New Equation ◽

Double Cross

Abstract Background Monitoring sodium intake through 24-h urine collection sample is recommended, but the implementation of this method can be difficult. The objective of this study was to develop and validate an equation using spot urine concentration to predict 24-h sodium excretion in the Malaysian population. Methods This was a Malaysian Community Salt Study (MyCoSS) sub-study, which was conducted from October 2017 to March 2018. Out of 798 participants in the MyCoSS study who completed 24-h urine collection, 768 of them have collected one-time spot urine the following morning. They were randomly assigned into two groups to form separate spot urine equations. The final spot urine equation was derived from the entire data set after confirming the stability of the equation by double cross-validation in both study groups. Newly derived spot urine equation was developed using the coefficients from the multiple linear regression test. A Bland-Altman plot was used to measure the mean bias and limits of agreement between estimated and measured 24-h urine sodium. The estimation of sodium intake using the new equation was compared with other established equations, namely Tanaka and INTERSALT. Results The new equation showed the least mean bias between measured and predicted sodium, − 0.35 (− 72.26, 71.56) mg/day compared to Tanaka, 629.83 (532.19, 727.47) mg/day and INTERSALT, and 360.82 (284.34, 437.29) mg/day. Predicted sodium measured from the new equation showed greater correlation with measured sodium (r = 0.50) compared to Tanaka (r =0.24) and INTERSALT (r = 0.44), P < 0.05. Conclusion Our newly developed equation from spot urine can predict least mean bias of sodium intake among the Malaysian population when 24-h urine sodium collection is not feasible.

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Comparison of two human infant urine collection methods for measuring estrone‐3‐glucuronide

American Journal of Physical Anthropology ◽

10.1002/ajpa.24299 ◽

2021 ◽

Author(s):

Carlye Chaney ◽

Margaret Corley ◽

Claudia Valeggia

Keyword(s):

Human Infant ◽

Urine Collection ◽

Collection Methods

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Concentrations of Seven Phthalate Monoesters in Infants and Toddlers Quantified in Urine Extracted from Diapers

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18136806 ◽

2021 ◽

Vol 18 (13) ◽

pp. 6806

Author(s):

Fiorella Lucarini ◽

Marc Blanchard ◽

Tropoja Krasniqi ◽

Nicolas Duda ◽

Gaëlle Bailat Rosset ◽

...

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Young Children ◽

Sampling Technique ◽

Gas Chromatography Mass Spectrometry ◽

Urine Collection ◽

Infants And Toddlers ◽

Urine Sampling ◽

Disposable Diapers ◽

Children’S Exposure

Carrying out exposure studies on children who are not toilet trained is challenging because of the difficulty of urine sampling. In this study, we optimized a protocol for urine collection from disposable diapers for the analysis of phthalate metabolites. The exposure of Swiss children (n = 113) between 6 months and 3 years of life to seven phthalates was assessed by gas chromatography–mass spectrometry measurements. The study showed limited exposures to phthalates, with only 22% of the samples containing some of the metabolites investigated. The three most frequently detected metabolites were monoethyl phthalate, mono-cyclohexyl phthalate, and mono-benzyl phthalate. We also detected mono-n-octyl phthalate and mono(3,5,5-trimethylhexyl) phthalate, which have rarely been observed in urine from infants and toddlers; therefore, di-n-octyl phthalate and bis(3,5,5-trimethylhexyl) phthalate can be considered as potentially new emerging phthalates. This study presents an initial snapshot of the Swiss children’s exposure to phthalates and provides a promising approach for further phthalate biomonitoring studies on young children using disposable diapers as urine sampling technique.

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