Faculty Opinions recommendation of Inhibition of IFN-gamma-inducible protein-10 abrogates colitis in IL-10-/- mice.

2003 ◽  
Author(s):  
Steve Ward
Keyword(s):  
Ifn Gamma ◽  
Inducible Protein

scholarly journals Inhibition of angiogenesis by interleukin-12 is mediated by the interferon-inducible protein 10

Blood ◽  
1996 ◽  
Vol 87 (9) ◽  
pp. 3877-3882 ◽  
Author(s):  
C Sgadari ◽  
AL Angiolillo ◽  
G Tosato
Keyword(s):  
Interleukin 12 ◽  
Human Interferon ◽  
Ifn Gamma ◽  
Mouse Splenocytes ◽  
Angiogenesis Inhibition ◽  
Inhibition Of Angiogenesis ◽  
Inducible Protein ◽  
Interferon Inducible Protein

Interleukin 12 (IL-12), a multifunctional cytokine produced by macrophages and B-cell lines, induces interferon-gamma (IFN-gamma) production, stimulates growth of both T and natural killer cells, promotes Th1-type helper T-cell responses, and inhibits neovascularization. Because the human interferon-inducible protein 10 (IP-10) can also inhibit neovascularization, we tested whether IP-10, induced by IL-12 through the intermediate IFN-gamma, might be a mediator of IL-12 angiogenesis inhibition. We report here that murine IL-12 profoundly inhibited basic fibroblast growth factor (bFGF)- induced Matrigel neovascularization in vivo, and that this effect of IL- 12 was neutralized by systemic administration of antibodies to either murine IFN-gamma or IP-10. Murine IL-12 induced murine IP-10 expression in mouse splenocytes, and human IFN-gamma induced human IP-10 expression in purified human endothelial cells, suggesting that IL-12 can induce IP-10 expression in certain cells. These results document the important role of IP-10 as a mediator of angiogenesis inhibition by IL-12, and raise the possibility that IP-10 may also contribute to the antitumor effect of IL-12.

scholarly journals Regulation of synthesis and turnover of an interferon-inducible mRNA.

1986 ◽  
Vol 6 (6) ◽  
pp. 2062-2067 ◽  
Author(s):  
J Kusari ◽  
G C Sen
Keyword(s):  
Actinomycin D ◽  
Ifn Gamma ◽  
Putative Protein ◽  
Ifn Alpha ◽  
Regulation Of Synthesis ◽  
Inducible Protein ◽  
High Level ◽  
Inhibitor Of Protein Synthesis ◽  
Interferon Inducible Protein ◽  
In Cells

Regulation of synthesis and turnover of an interferon (IFN)-inducible mRNA, mRNA 561, in HeLa monolayer cells was studied. Cytoplasmic levels of this mRNA were estimated by hybridization analyses with a cDNA clone that we have isolated as a probe. IFN-alpha A induced a high level of this mRNA in a transient fashion, whereas no induction was observed in response to IFN-gamma. Surprisingly little mRNA 561 was induced in cells treated simultaneously with IFN-alpha A and an inhibitor of protein synthesis, suggesting that in addition to IFN-alpha A, an interferon-inducible protein was needed for induction of this mRNA. Apparently this putative protein could be induced by IFN-gamma as well. Thus, although little mRNA 561 was synthesized in cells treated either with IFN-gamma alone or with IFN-alpha A and cycloheximide, a large quantity of this mRNA was induced in cells which had been pretreated with IFN-gamma and then treated with IFN-alpha A and cycloheximide. Once mRNA 561 was induced by IFN-alpha A, it turned over rapidly. This rapid turnover could be blocked by actinomycin D or cycloheximide indicating that another IFN-inducible protein may mediate this process.

scholarly journals Biochemical characterization of a gamma interferon-inducible cytokine (IP-10).

1987 ◽  
Vol 166 (4) ◽  
pp. 1084-1097 ◽  
Author(s):  
A D Luster ◽  
J V Ravetch
Keyword(s):  
Biochemical Characterization ◽  
Delayed Type Hypersensitivity ◽  
Hypersensitivity Response ◽  
Ifn Gamma ◽  
Primary Cell Lines ◽  
Inducible Protein ◽  
Monospecific Antisera ◽  
Delayed Type Hypersensitivity Response

An IFN-gamma-inducible protein, IP-10, has previously been described to belong to a gene family of chemotactic and mitogenic proteins, associated with inflammation and proliferation. Biochemical characterization of this predicted protein has been pursued through the development of polyclonal monospecific antisera to recombinant protein and synthetic peptides. These reagents establish that the IP-10 protein is secreted from a variety of cells (endothelial, monocyte, fibroblast, and keratinocyte) in response to IFN-gamma. Posttranslational processing occurs in the biosynthesis of this protein, resulting in a 6-7-kD species, which may reflect COOH-terminal cleavage. Pulse-chase studies indicate that this processing is a rapid event in the primary cell lines studied, completed in the 30-min labeling period. A model is presented for the processing and secondary structure of this protein. In an accompanying study, Kaplan, et al. using these antisera, demonstrate that the IP-10 protein is associated, in vivo, with a delayed-type hypersensitivity response.

Regulation of synthesis and turnover of an interferon-inducible mRNA

1986 ◽  
Vol 6 (6) ◽  
pp. 2062-2067
Author(s):  
J Kusari ◽  
G C Sen
Keyword(s):  
Actinomycin D ◽  
Ifn Gamma ◽  
Putative Protein ◽  
Ifn Alpha ◽  
Regulation Of Synthesis ◽  
Inducible Protein ◽  
High Level ◽  
Inhibitor Of Protein Synthesis ◽  
Interferon Inducible Protein ◽  
In Cells

Regulation of synthesis and turnover of an interferon (IFN)-inducible mRNA, mRNA 561, in HeLa monolayer cells was studied. Cytoplasmic levels of this mRNA were estimated by hybridization analyses with a cDNA clone that we have isolated as a probe. IFN-alpha A induced a high level of this mRNA in a transient fashion, whereas no induction was observed in response to IFN-gamma. Surprisingly little mRNA 561 was induced in cells treated simultaneously with IFN-alpha A and an inhibitor of protein synthesis, suggesting that in addition to IFN-alpha A, an interferon-inducible protein was needed for induction of this mRNA. Apparently this putative protein could be induced by IFN-gamma as well. Thus, although little mRNA 561 was synthesized in cells treated either with IFN-gamma alone or with IFN-alpha A and cycloheximide, a large quantity of this mRNA was induced in cells which had been pretreated with IFN-gamma and then treated with IFN-alpha A and cycloheximide. Once mRNA 561 was induced by IFN-alpha A, it turned over rapidly. This rapid turnover could be blocked by actinomycin D or cycloheximide indicating that another IFN-inducible protein may mediate this process.

Serum interferon-gamma (IFN-gamma) in chronic oral candidosis

1998 ◽  
Vol 36 (5) ◽  
pp. 269-273
Author(s):  
SZKARADKIEWICZ ◽  
SZPONAR ◽  
KRZEMINSKA-JASKOWIAK ◽  
TUECKA
Keyword(s):  
Interferon Gamma ◽  
Ifn Gamma ◽  
Oral Candidosis
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