Faculty Opinions recommendation of The integral membrane enzyme PagP alternates between two dynamically distinct states.

AbstractCampylobacter spp. enteritis is the most frequent bacterial enteritis in both adults and children and is sometimes a source of severe complications. Its diagnosis by culture suffers from a lack of sensitivity and delays the result, preventing an early initiation of optimal antibiotic therapy in some cases. Our aim was to test a new rapid immuno-enzymatic method for Campylobacter spp. diagnosis in comparison to a composite reference standard (CRS). Stool samples from the French National Reference Center for Campylobacter and Helicobacter were tested with the CAMPYLOBACTER QUIK CHEK™ (Abbott). The CRS used to consider a case positive for Campylobacter spp. was a positive culture and, in case of a negative culture, a positive result obtained with both an ELISA and a molecular test. One hundred and eight stools were included: 53 were positive according to the CRS. If performed alone, culture would have missed 5 cases which the CAMPYLOBACTER QUIK CHEK™ detected. Finally, the CAMPYLOBACTER QUIK CHEK™ showed a sensitivity of 96.2% and a specificity of 94.5% and is relevant for clinical practice. Given the characteristics of the new method, it can be used as a screening method for Campylobacter spp. detection.

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Comparison of pH-Membrane Enzyme Electrodes for Urea with Covalently Bound Enzyme.

Analytical Letters ◽

10.1080/00032719408001088 ◽

1994 ◽

Vol 27 (3) ◽

pp. 475-486 ◽

Cited By ~ 20

Author(s):

Robert Koncki ◽

Ewa Kopczewska ◽

Stanisław Glab

Keyword(s):

Enzyme Electrodes ◽

Membrane Enzyme ◽

Covalently Bound

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Incidence and distribution of Sweetpotato viruses and their implication on sweetpotato seed system in Malawi

Journal of Plant Pathology ◽

10.1007/s42161-021-00830-4 ◽

2021 ◽

Author(s):

Willard Mbewe ◽

Andrew Mtonga ◽

Margret Chiipanthenga ◽

Kennedy Masamba ◽

Gloria Chitedze ◽

...

Keyword(s):

Sweet Potato ◽

Virus Disease ◽

Disease Incidence ◽

Mottle Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Foliar Symptoms ◽

Membrane Enzyme ◽

Sweet Potato Virus ◽

Seed System ◽

Clean Seed

AbstractA survey was carried out in 19 districts to investigate the prevalence and distribution of sweetpotato virus disease (SPVD) and its implication on the sustainability of clean seed system in Malawi. A total of 166 leaf samples were collected and tested for the presence of 8 viruses using nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). SPVD foliar symptoms were observed in 68.42% of the surveyed districts. There were significant variations in disease incidence and severity (p < 0.001) among districts, with the highest incidence in Mulanje (28.34%). Average SPVD severity score was 3.05. NCM-ELISA detected sweet potato feathery mottle virus (SPFMV, 30.54%), sweet potato mild mottle virus (SPMMV, 31.14%), sweet potato mild speckling virus (SPMSV, 16.17%), sweet potato C-6 virus (SPC6V, 13.77%), sweet potato chlorotic stunt virus (SPCSV, 22.16%), sweet potato collusive virus (SPCV, 30.54%), sweet potato virus G (SPVG, 11.38%), cucumber mosaic virus (CMV, 7.78%) either in single or mixed infections. Data from this study indicate a significant SPVD occurrence in the country, and the consequence implications towards national sweetpotato seed system.

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