Faculty Opinions recommendation of Heritable variation for female remating in Lobesia botrana, a usually monandrous moth.

AbstractChronic kidney disease (CKD), which can ultimately progress to kidney failure, is influenced by genetics and the environment. Genes identified in human genome wide association studies (GWAS) explain only a small proportion of the heritable variation and lack functional validation, indicating the need for additional model systems. Outbred heterogeneous stock (HS) rats have been used for genetic fine-mapping of complex traits, but have not previously been used for CKD traits. We performed GWAS for urinary protein excretion (UPE) and CKD related serum biochemistries in 245 male HS rats. Quantitative trait loci (QTL) were identified using a linear mixed effect model that tested for association with imputed genotypes. Candidate genes were identified using bioinformatics tools and targeted RNAseq followed by testing in a novel in vitro model of human tubule, hypoxia-induced damage. We identified two QTL for UPE and five for serum biochemistries. Protein modeling identified a missense variant within Septin 8 (Sept8) as a candidate for UPE. Sept8/SEPTIN8 expression increased in HS rats with elevated UPE and tubulointerstitial injury and in the in vitro hypoxia model. SEPTIN8 is detected within proximal tubule cells in human kidney samples and localizes with acetyl-alpha tubulin in the culture system. After hypoxia, SEPTIN8 staining becomes diffuse and appears to relocalize with actin. These data suggest a role of SEPTIN8 in cellular organization and structure in response to environmental stress. This study demonstrates that integration of a rat genetic model with an environmentally induced tubule damage system identifies Sept8/SEPTIN8 and informs novel aspects of the complex gene by environmental interactions contributing to CKD risk.

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Organic Farming and Cover-Crop Management Reduce Pest Predation in Austrian Vineyards

Insects ◽

10.3390/insects12030220 ◽

2021 ◽

Vol 12 (3) ◽

pp. 220

Author(s):

Jo Marie Reiff ◽

Sebastian Kolb ◽

Martin H. Entling ◽

Thomas Herndl ◽

Stefan Möth ◽

...

Keyword(s):

Organic Farming ◽

Pest Control ◽

Cover Crops ◽

Cover Crop ◽

Management Practices ◽

Integrated Management ◽

Lobesia Botrana ◽

Management Options ◽

Natural Pest Control ◽

Predation Rates

Habitat simplification and intensive use of pesticides are main drivers of global arthropod declines and are, thus, decreasing natural pest control. Organic farming, complex landscapes, and local vineyard management practices such as implementation of flower-rich cover-crop mixtures may be a promising approach to enhance predator abundance and, therefore, natural pest control. We examined the effect of organic versus integrated management, cover-crop diversity in the vineyard inter-rows, and landscape composition on the natural pest control of Lobesia botrana eggs and pupae. Predation of L. botrana pupae was reduced by organic farming and species-poor cover-crops by about 10%. Predation rates of L. botrana eggs did not differ significantly in any of the studied management options. Dominant predators were earwigs (Forficulidae), bush crickets (Tettigoniidae), and ants (Formicidae). Negative effects of organic viticulture are most likely related to the negative nontarget effects on arthropods related to the frequent sulfur and copper applications in combination with the avoidance of strongly damaging insecticides by integrated winegrowers. While a 10% difference in predation rates on a single pest stage is unlikely to have strong practical implications, our results show that the assumed effectiveness of environmentally friendly agriculture needs to be evaluated for specific crops and regions.

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Models Applied to Grapevine Pests: A Review

Insects ◽

10.3390/insects12020169 ◽

2021 ◽

Vol 12 (2) ◽

pp. 169

Author(s):

Federico Lessio ◽

Alberto Alma

Keyword(s):

Lobesia Botrana ◽

Grape Berry ◽

Homalodisca Vitripennis ◽

Flavescence Dorée ◽

Or Groups ◽

Scaphoideus Titanus ◽

Different Types ◽

Computer Based ◽

Biological Aspects ◽

Definition Of

This paper reviews the existing predictive models concerning insects and mites harmful to grapevine. A brief conceptual description is given on the definition of a model and about different types of models: deterministic vs. stochastics, continuous vs. discrete, analytical vs. computer-based, and descriptive vs. data-driven. The main biological aspects of grapevine pests covered by different types of models are phenology, population growth and dynamics, species distribution, and invasion risk. A particular emphasis is put on forecasting epidemics of plant disease agents transmitted by insects with sucking-piercing mouthparts. The most investigated species or groups are the glassy-winged sharpshooter Homalodisca vitripennis (Germar) and other vectors of Xylella fastidiosa subsp. fastidiosa, a bacterium agent of Pierce’s disease; the European grape berry moth, Lobesia botrana (Denis and Schiffermuller); and the leafhopper Scaphoideus titanus Ball, the main vector of phytoplasmas agents of Flavescence dorée. Finally, the present and future of decision-support systems (DSS) in viticulture is discussed.

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Male-derived copulatory plugs enhance implantation success in female Mus musculus†

Biology of Reproduction ◽

10.1093/biolre/ioaa228 ◽

2020 ◽

Author(s):

Lough-Stevens Michael ◽

Caleb Ghione ◽

Matthew Urness ◽

Adelaide Hobbs ◽

Colleen Sweeney ◽

...

Keyword(s):

Mus Musculus ◽

Genetically Engineered ◽

Fertilization Rates ◽

Copulatory Plug ◽

Female Remating ◽

Implantation Rates ◽

Transfer Method ◽

Copulatory Plugs ◽

Threshold Amount ◽

Implantation Success

Abstract Among a wide diversity of sexually reproducing species, male ejaculates coagulate to form what has been termed a copulatory plug. A number of functions have been attributed to copulatory plugs, including, but not limited to, the inhibition of female remating and the promotion of ejaculate movement. Here we demonstrate that copulatory plugs also influence the likelihood of implantation, which occurs roughly four days after copulation in mice. Using a bead transfer method to control for differences in ejaculate retention and fertilization rates, we show that implantation rates significantly drop among females mated to genetically engineered males incapable of forming plugs (because they lack functional TGM4, the main enzyme responsible for its formation). Surprisingly, this result does not correlate with differences in circulating progesterone levels among females, an important hormone involved in implantation. In this paper we discuss three models that connect male-derived copulatory plugs to implantation success, including the hypothesis that plugs contribute to a threshold amount of stimulation required for females to become receptive to implantation.

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Grape variety affects larval performance and also female reproductive performance of the European grapevine moth Lobesia botrana (Lepidoptera: Tortricidae)

Bulletin of Entomological Research ◽

10.1079/ber2005417 ◽

2006 ◽

Vol 96 (2) ◽

pp. 205-212 ◽

Cited By ~ 54

Author(s):

J. Moreau ◽

B. Benrey ◽

D. Thiéry

Keyword(s):

Host Plant ◽

Adult Emergence ◽

Lobesia Botrana ◽

Larval Performance ◽

European Grapevine Moth ◽

Wild Grape ◽

Larval Host ◽

Fitness Index ◽

Grapevine Moth

AbstractFor insect herbivores, the quality of the larval host plant is a key determinant of fitness. Therefore, insect populations are supposed to be positively correlated with the nutritional quality of their host plant. This study aimed to determine if and how different varieties of grapes (including the wild grape Lambrusque) affect both larval and adult performance of the polyphagous European grapevine moth Lobesia botrana (Denis & Schiffermüller). Significant differences were found in larval development time, but not in pupal mass, adult emergence rate, or sex ratio. Although the fecundity of females is not different among varieties, females fed on some varieties produced eggs of different sizes which are correlated to their fertility. Thus, females adapt resource allocation to eggs depending on their diet as larvae. Using a fitness index, the average reproductive output was found to be highest for females reared on cv. Chardonnay. Females reared on wild grape produced a fitness index identical to the cultivated grapes. However, Lambrusque and Gewurztraminer separate themselves from the cultivated varieties according to our discriminant analyses. It is emphasized, through this study, that cultivars fed on by larvae should be considered in the population dynamics of L. botrana and that egg number is insufficient to determine host plant quality.

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