Faculty Opinions recommendation of Crucial roles of Foxa2 in mouse anterior-posterior axis polarization via regulation of anterior visceral endoderm-specific genes.

2007 ◽  
Author(s):  
Patrick Tam
Keyword(s):  
Visceral Endoderm ◽  
Anterior Posterior ◽  
Anterior Posterior Axis ◽  
Anterior Visceral Endoderm

HNF3beta and Lim1 interact in the visceral endoderm to regulate primitive streak formation and anterior-posterior polarity in the mouse embryo

Development ◽  
1999 ◽  
Vol 126 (20) ◽  
pp. 4499-4511 ◽  
Author(s):  
A. Perea-Gomez ◽  
W. Shawlot ◽  
H. Sasaki ◽  
R.R. Behringer ◽  
S. Ang
Keyword(s):  
Mouse Embryo ◽  
Primitive Streak ◽  
Axis Formation ◽  
Visceral Endoderm ◽  
Primary Defect ◽  
Homozygous Mutant ◽  
Early Mouse ◽  
Mesoderm Patterning ◽  
Anterior Posterior ◽  
Anterior Visceral Endoderm

Recent embryological and genetic experiments have suggested that the anterior visceral endoderm and the anterior primitive streak of the early mouse gastrula function as head- and trunk-organising centers, respectively. Here, we report that HNF3beta and Lim1 are coexpressed in both organising centers suggesting synergistic roles of these genes in regulating organiser functions and hence axis development in the mouse embryo. To investigate this possibility, we generated compound HNF3beta and Lim1 mutant embryos. An enlarged primitive streak and a lack of axis formation were observed in HNF3beta (−)(/)(−);Lim1(−)(/)(−), but not in single homozygous mutant embryos. Chimera experiments indicate that the primary defect in these double homozygous mutants is due to loss of activity of HNF3beta and Lim1 in the visceral endoderm. Altogether, these data provide evidence that these genes function synergistically to regulate organiser activity of the anterior visceral endoderm. Moreover, HNF3beta (−)(/)(−);Lim1(−)(/)(−) mutant embryos also exhibit defects in mesoderm patterning that are likely due to lack of specification of anterior primitive streak cells.

scholarly journals Canonical Wnt Signaling and Its Antagonist Regulate Anterior-Posterior Axis Polarization by Guiding Cell Migration in Mouse Visceral Endoderm

2005 ◽  
Vol 9 (5) ◽  
pp. 639-650 ◽  
Author(s):  
Chiharu Kimura-Yoshida ◽  
Hiroshi Nakano ◽  
Daiji Okamura ◽  
Kazuki Nakao ◽  
Shigenobu Yonemura ◽  
...  
Keyword(s):  
Cell Migration ◽  
Wnt Signaling ◽  
Visceral Endoderm ◽  
Canonical Wnt Signaling ◽  
Canonical Wnt ◽  
Anterior Posterior ◽  
Anterior Posterior Axis

scholarly journals Heading forwards: anterior visceral endoderm migration in patterning the mouse embryo

2014 ◽  
Vol 369 (1657) ◽  
pp. 20130546 ◽  
Author(s):  
Matthew J. Stower ◽  
Shankar Srinivas
Keyword(s):  
Epithelial Cells ◽  
Early Development ◽  
Mouse Embryo ◽  
Visceral Endoderm ◽  
Coordinated Action ◽  
Specialized Population ◽  
The Brain ◽  
Anterior Posterior ◽  
Anterior Visceral Endoderm

The elaboration of anterior–posterior (A–P) pattern is one of the earliest events during development and requires the precisely coordinated action of several players at the level of molecules, cells and tissues. In mammals, it is controlled by a specialized population of migratory extraembryonic epithelial cells, the anterior visceral endoderm (AVE). The AVE is a signalling centre that is responsible for several important patterning events during early development, including specifying the orientation of the A–P axis and the position of the heart with respect to the brain. AVE cells undergo a characteristic stereotypical migration which is crucial to their functions.

scholarly journals Requirement for β-Catenin in Anterior-Posterior Axis Formation in Mice

2000 ◽  
Vol 148 (3) ◽  
pp. 567-578 ◽  
Author(s):  
Joerg Huelsken ◽  
Regina Vogel ◽  
Volker Brinkmann ◽  
Bettina Erdmann ◽  
Carmen Birchmeier ◽  
...  
Keyword(s):  
Wnt Pathway ◽  
Wnt Signaling Pathway ◽  
Primitive Streak ◽  
Mouse Embryos ◽  
Axis Formation ◽  
Deficient Mouse ◽  
Visceral Endoderm ◽  
Embryonic Ectoderm ◽  
Anterior Posterior ◽  
Anterior Posterior Axis

The anterior-posterior axis of the mouse embryo is defined before formation of the primitive streak, and axis specification and subsequent anterior development involves signaling from both embryonic ectoderm and visceral endoderm. Τhe Wnt signaling pathway is essential for various developmental processes, but a role in anterior-posterior axis formation in the mouse has not been previously established. β-Catenin is a central player in the Wnt pathway and in cadherin-mediated cell adhesion. We generated β-catenin–deficient mouse embryos and observed a defect in anterior-posterior axis formation at embryonic day 5.5, as visualized by the absence of Hex and Hesx1 and the mislocation of cerberus-like and Lim1 expression. Subsequently, no mesoderm and head structures are generated. Intercellular adhesion is maintained since plakoglobin substitutes for β-catenin. Our data demonstrate that β-catenin function is essential in anterior-posterior axis formation in the mouse, and experiments with chimeric embryos show that this function is required in the embryonic ectoderm.

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