Faculty Opinions recommendation of Identification and characterization of Escherichia coli thioesterase III that functions in fatty acid beta-oxidation.

2008 ◽  
Author(s):  
Wolfgang Buckel
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Beta Oxidation ◽  
Fatty Acid Beta Oxidation ◽  
Identification And Characterization

BIOSYNTHESIS OF BUTYRIC ACID FROM GLUCOSE THROUGH THE INVERTED FATTY ACID BETA-OXIDATION PATHWAY BY RECOMBINANT ESCHERICHIA COLI STRAIN IN BIOCATALYTIC MODE DUE TO ENFORCED ATP HYDROLYSIS

2021 ◽  
Vol 1 (19) ◽  
pp. 268-269
Author(s):  
A.Yu. Skorokhodova ◽  
V.G. Debabov
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Butyric Acid ◽  
Atp Hydrolysis ◽  
Coli Strain ◽  
Recombinant Escherichia Coli ◽  
Beta Oxidation ◽  
Oxidation Pathway ◽  
Full Cell ◽  
Fatty Acid Beta Oxidation

The feasibility of the application of enforced ATP hydrolysis to ensure anaerobic functioning of Escherichia coli strain producing butyric acid through the inverted fatty acid beta-oxidation pathway as a full-cell biocatalyst has been demonstrated.

BIOSYNTHESIS OF 3-HYDROXYFUNCTIONALIZED C4-C8 ALIPHATIC CARBOXYLIC ACID FROM GLUCOSE THROUGH INVERTED FATTY ACID BETA- OXIDATION PATHWAY BY RECOMBINANT ESCHERICHIA COLI STRAINS

2020 ◽  
pp. 10-11
Author(s):  
A. Skorokhodova ◽  
V. Debabov
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Carboxylic Acid ◽  
Recombinant Escherichia Coli ◽  
Beta Oxidation ◽  
Aliphatic Carboxylic Acid ◽  
Oxidation Pathway ◽  
Fatty Acid Beta Oxidation

The feasibility of the biosynthesis from glucose of 3-hydroxyfunctionalized C4-C8 carboxylates upon the reversal of the fatty acid beta-oxidation in recombinant Escherichia coli strains has been demonstrated.

Study of the Potential of Reversal of the Fatty Acid Beta-Oxidation Pathway for Stereoselective Biosynthesis of (S)-1,3-Butanediol from Glucose by Recombinant Escherichia coli Strains

2019 ◽  
Vol 35 (5) ◽  
pp. 12-19
Author(s):  
A.Yu. Skorokhodova ◽  
V.G. Debabov
Keyword(s):  
Escherichia Coli ◽  
Fatty Acid ◽  
Essential Gene ◽  
Coli Strain ◽  
Recombinant Escherichia Coli ◽  
Beta Oxidation ◽  
Oxidation Pathway ◽  
Gene Coding ◽  
Fundamental Research ◽  
Fatty Acid Beta Oxidation

A possible contribution of collateral enzymes to the formation of key precursor metabolite, 3-hydroxybutyryl-CoA, in a recombinant Escherichia coli strain engineered for 1,3-butanediol biosynthesis from glucose through the inverted fatty acid beta-oxidation pathway has been evaluated. The inactivation of the 3-hydroxyadipyl-CoA dehydrogenase gene, paaH, did not prevent the 1,3-butanol biosynthesis during anaerobic glucose utilization by the strain with the intact essential gene fabG coding for 3-ketoacyl-ACP reductase, which can catalyze the conversion of acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA. The subsequent inactivation in the strain of fadB gene coding for (S)-stereospecific 3-hydroxyacyl-CoA dehydrogenase of the fatty acid beta-oxidation led to the abolishment of the 1,3-butanediol synthesis. The respective diol was also not found among the products secreted by the strain possessing the intact fabG and paaH genes upon an individual deletion of fadB gene. It was established that the collateral enzymes did not participate in the formation of 3-hydroxybutyryl-CoA in the studied strains and the respective CoA-derivative was synthesized solely by the (S)-specific enzyme of the fatty acid beta-oxidation pathway. The obtained results indicate that the reversal of the fatty acid beta-oxidation pathway can ensure the enantioselective biosynthesis of the (S)-stereoisomer of 1,3-butanediol in engineered E. coli strains. 1,3-butanediol, fatty acid beta-oxidation, Escherichia coli, glucose, metabolic engineering, stereoisomer. The work was carried out with financial support Russian Foundation for Fundamental Research (No. 18-29-08059).

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