Faculty Opinions recommendation of Lipidic cubic phase technologies for membrane protein structural studies.

2016 ◽  
Author(s):  
Louis De Felice
Keyword(s):  
Membrane Protein ◽  
Cubic Phase ◽  
Structural Studies ◽  
Lipidic Cubic Phase

An efficient nanolitre-volume multi-channel device for highly viscous materials used in membrane protein crystallization

2013 ◽  
Vol 46 (3) ◽  
pp. 829-831
Author(s):  
Jinghui Luo ◽  
Raphaël Zwier ◽  
Jan Pieter Abrahams
Keyword(s):  
Lower Bound ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Crystal Structures ◽  
Protein Crystallization ◽  
Cubic Phase ◽  
Operation Speed ◽  
Membrane Protein Crystallization ◽  
Lipidic Cubic Phase ◽  
Materials Used

The crystal structures of various important membrane proteins could not have been solved without lipidic cubic phase (LCP) crystallization, and yet, compared to traditionalin surfocrystallization, LCP crystallization is not widely used because its extreme viscosity makes the cubic phase difficult to handle. Robots that can dispense LCPs are very specialized and therefore very expensive. Here, an accurate multi-channel device is described. It dispenses LCPs onto glass plates down to volumes of 20 nl accuracy and has an accuracy of 10% when dispensing 200 nl – the lower bound of LCP volumes dispensed for crystallization trials. Because of its multi-channel tips, operation speed goes up by a factor of four compared to simpler devices. It can be operated by hand, but its design also allows it to be built into a basic dispensing robot. Thus, the device lowers the threshold for LCP crystallization of membrane proteins/peptides.

scholarly journals Lipidic cubic phase injector facilitates membrane protein serial femtosecond crystallography

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Uwe Weierstall ◽  
Daniel James ◽  
Chong Wang ◽  
Thomas A. White ◽  
Dingjie Wang ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Cubic Phase ◽  
Lipidic Cubic Phase ◽  
Serial Femtosecond Crystallography

scholarly journals Usefulness of oils for cleaning the host matrix and for cryoprotection of lipidic cubic phase crystals

2019 ◽  
Vol 52 (4) ◽  
pp. 864-868
Author(s):  
Satomi Niwa ◽  
Kazuki Takeda
Keyword(s):  
Data Collection ◽  
Membrane Protein ◽  
Crystal Surface ◽  
Phase Method ◽  
Cubic Phase ◽  
Data Sets ◽  
Accurate Data ◽  
Host Matrix ◽  
Crystal Stability ◽  
Lipidic Cubic Phase

The lipidic cubic phase method is an effective approach for membrane protein crystallography. The in meso grown crystals are usually cryocooled directly without removing the host matrix from the harvested crystal surface. However, the host matrix often causes the appearance of scattering rings and an increase in background scattering during the data collection. Moreover, the frozen host matrix sometimes becomes opaque and it can hinder conventional crystal centering. In this study, several oils were examined for their ability to clean the host matrix and to provide cryoprotection for crystals grown in the lipidic cubic phase. Several of the tested oils appeared to be useful in terms of their effect on crystal stability and background scattering. This method should be of value for the collection of highly accurate data sets.

scholarly journals Vitamin E-based glycoside amphiphiles for membrane protein structural studies

2018 ◽  
Vol 16 (14) ◽  
pp. 2489-2498 ◽  
Author(s):  
Muhammad Ehsan ◽  
Yang Du ◽  
Iago Molist ◽  
Alpay B. Seven ◽  
Parameswaran Hariharan ◽  
...  
Keyword(s):  
Vitamin E ◽  
Membrane Protein ◽  
G Protein ◽  
Structural Studies ◽  
G Protein Coupled Receptor ◽  
G Protein Coupled ◽  
Novel Agent

A vitamin E-based novel agent (i.e., VEG-3) was markedly effective at stabilizing and visualizing a G-protein coupled receptor (GPCR)-Gs complex.

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