scholarly journals Design of Temperature-Responsive Cell Culture Surfaces for Cell Sheet Engineering

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Y. Akiyama
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Cell Sheet ◽  
Extensive Study ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Interdisciplinary Field ◽  
Culture Surface ◽  
Responsive Cell

Temperature-responsive cell culture surfaces, which modulate cell attachment/detachment characteristics with temperature, have been used to fabricate cell sheets. Extensive study on fabrication of cell sheet with the temperature-responsive cell culture surface, manipulation, and transplantation of the cell sheet has established the interdisciplinary field of cell sheet engineering, in which engineering, biological, and medical fields closely collaborate. Such collaboration has pioneered cell sheet engineering, making it a promising and attractive technology in tissue engineering and regenerative medicine. This review introduces concepts of cell sheet engineering, followed by designs for the fabrication of various types of temperature-responsive cell culture surfaces and technologies for cell sheet manipulation. The development of various methods for the fabrication of temperature-responsive cell culture surfaces was also summarized. The availability of cell sheet engineering for the treatment and regeneration of damaged human tissue has also been described, providing examples of the clinical application of cell sheet transplantation in humans.

scholarly journals Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Mitsuyoshi Nakao ◽  
Kyungsook Kim ◽  
Kenichi Nagase ◽  
David W. Grainger ◽  
Hideko Kanazawa ◽  
...  
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Sheet ◽  
Cellular Structures ◽  
Phenotypic Traits ◽  
Cell Sheets ◽  
Temperature Responsive ◽  
Cell Sheet Technology ◽  
Responsive Cell

Abstract Background In most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and function. MSC therapeutic efficacies are inconsistent, largely attributed to transplanted MSC difficulties both in engrafting at tissue sites and in retaining their therapeutic functions from suspension formulations. MSC functional components, including cell adhesion and cell–cell junction proteins, and ECM that contribute to essential cellular therapeutic effects, are damaged or removed by proteolytic enzymes used in stem cell harvesting strategies from culture. To overcome these limitations, methods to harvest and transplant cells without disrupting critical stem cell functions are required. Cell sheet technology, exploiting temperature-responsive cell culture surfaces, permits cell harvest without cell protein damage. This study is focused on phenotypic traits of MSC sheets structurally and functionally to understand therapeutic benefits of cell sheets. Methods/results This study verified cleaved cellular proteins (vinculin, fibronectin, laminin, integrin β-1, and connexin 43) and increased apoptotic cell death produced under standard trypsin harvesting treatment in a time-dependent manner. However, MSC sheets produced without trypsin using only temperature-controlled sheet harvest from culture plastic exhibited intact cellular structures. Also, MSCs harvested using enzymatic treatment (i.e., chemical disruption) showed higher pYAP expression compared to MSC sheets. Conclusion Retention of cellular structures such as ECM, cell–cell junctions, and cell–ECM junctions is correlated with human umbilical cord mesenchymal stem cell (hUC-MSC) survival after detachment from cell culture surfaces. Retaining these proteins intact in MSC cultures using cell sheet technology is proposed to enhance stem cell survival and their function in stem cell-based therapy.

Temperature-responsive cell culture surfaces for regenerative medicine with cell sheet engineering

2007 ◽  
Vol 32 (8-9) ◽  
pp. 1123-1133 ◽  
Author(s):  
Masayuki Yamato ◽  
Yoshikatsu Akiyama ◽  
Jun Kobayashi ◽  
Joseph Yang ◽  
Akihiko Kikuchi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Regenerative Medicine ◽  
Cell Sheet ◽  
Cell Sheet Engineering ◽  
Temperature Responsive ◽  
Responsive Cell

Preparation of Poly(N-isopropylacrylamide) Grafted Polydimethylsiloxane by Using Electron Beam Irradiation

2013 ◽  
Vol 25 (4) ◽  
pp. 631-636 ◽  
Author(s):  
Yoshikatsu Akiyama ◽  
◽  
Masayuki Yamato ◽  
Teruo Okano
Keyword(s):  
Cell Culture ◽  
Electron Beam ◽  
Cell Attachment ◽  
Pdms Surface ◽  
Chemical Treatments ◽  
Temperature Responsive ◽  
Treatment Conditions ◽  
Culture Surface ◽  
Surface Modified ◽  
Responsive Cell

A poly(N-isopropylacrylamide) (PIPAAm) grafted poly(dimethylsiloxane) (PDMS) surface was prepared as a temperature-responsive cell culture surface by using electron beam (EB) irradiation. Different chemical treatments to modify the bare PDMS surface were investigated for subsequent grafting of PIPAAm, and treatment conditions were optimized to prepare the temperature-responsive cell culture surface. The PDMS surface was initially activated to form silanol groups with conventional O2 plasma or hydrochloric acid (HCl) treatment. Activated PDMS surfaces were individually immobilized with three different conventional silane compounds, i.e., 3-mercaptopropyltrimethoxysilane (MerTMS), 3-methacryloxypropyltrimethoxysilane (MetTMS), and 3-aminopropyltrimethoxysilane (AmiTMS). O2 plasma treatment made PDMS more hydrophilic. In contrast, PDMS surfaces activated with HCl treatment were relatively hydrophobic. Observation of the activated PDMS surface modified with MerTMS, MetTMS, and AmiTMS indicated that these silane compounds had been favorably immobilized on plasma-treated PDMS surfaces. FT-IR/ATR analysis demonstrated that immobilized silane compounds enabled PIPAAm grafting on the PDMS surface. Cell attachment and detachment analysis also suggested that the PDMS surface sequentially treated with O2 plasma and AmiTMS compound was a substrate appropriate for preparing a temperature-responsive cell culture surface by EB irradiation-induced PIPAAm grafting method. The intelligent surface may further be applied to mechanically stretchable temperature-responsive cell culture surfaces.

scholarly journals Recent Advances in ROS-Responsive Cell Sheet Techniques for Tissue Engineering

2019 ◽  
Vol 20 (22) ◽  
pp. 5656 ◽  
Author(s):  
Min-Ah Koo ◽  
Mi Hee Lee ◽  
Jong-Chul Park
Keyword(s):  
Tissue Engineering ◽  
Cell Sheet ◽  
Cell Detachment ◽  
Oxygen Species ◽  
Cell Sheets ◽  
Cell Sheet Engineering ◽  
New Approach ◽  
Responsive Systems ◽  
Cell Based Therapy ◽  
Responsive Cell

Cell sheet engineering has evolved rapidly in recent years as a new approach for cell-based therapy. Cell sheet harvest technology is important for producing viable, transplantable cell sheets and applying them to tissue engineering. To date, most cell sheet studies use thermo-responsive systems to detach cell sheets. However, other approaches have been reported. This review provides the progress in cell sheet detachment techniques, particularly reactive oxygen species (ROS)-responsive strategies. Therefore, we present a comprehensive introduction to ROS, their application in regenerative medicine, and considerations on how to use ROS in cell detachment. The review also discusses current limitations and challenges for clarifying the mechanism of the ROS-responsive cell sheet detachment.

Bulk poly(N-isopropylacrylamide) (PNIPAAm) thermoresponsive cell culture platform: toward a new horizon in cell sheet engineering

2019 ◽  
Vol 7 (6) ◽  
pp. 2277-2287 ◽  
Author(s):  
Andrew Choi ◽  
Kyoung Duck Seo ◽  
Hyungjun Yoon ◽  
Seon Jin Han ◽  
Dong Sung Kim
Keyword(s):  
Cell Culture ◽  
Cell Sheet ◽  
Cell Sheets ◽  
Cell Sheet Engineering

In contrast to the conventional ‘grafting’-based thermoresponsive cell culture platform, we first developed a bulk form of thermoresponsive cell culture platform for attaching/detaching diverse types and origins of the cell sheets in different shape.

scholarly journals Temperature-responsive intelligent interfaces for biomolecular separation and cell sheet engineering

2009 ◽  
Vol 6 (suppl_3) ◽  
Author(s):  
Kenichi Nagase ◽  
Jun Kobayashi ◽  
Teruo Okano
Keyword(s):  
Cell Sheet ◽  
Copolymer Composition ◽  
Cell Sheets ◽  
Chromatographic Separations ◽  
Cell Sheet Engineering ◽  
External Temperature ◽  
Cell Monolayers ◽  
Temperature Responsive ◽  
Confluent Cell ◽  
Dependent Cell

Temperature-responsive intelligent surfaces, prepared by the modification of an interface with poly( N -isopropylacrylamide) and its derivatives, have been used for biomedical applications. Such surfaces exhibit temperature-responsive hydrophilic/hydrophobic alterations with external temperature changes, which, in turn, result in thermally modulated interactions with biomolecules and cells. In this review, we focus on the application of these intelligent surfaces to chromatographic separation and cell cultures. Chromatographic separations using several types of intelligent surfaces are mentioned briefly, and various effects related to the separation of bioactive compounds are discussed, including wettability, copolymer composition and graft polymer architecture. Similarly, we also summarize temperature-responsive cell culture substrates that allow the recovery of confluent cell monolayers as contiguous living cell sheets for tissue-engineering applications. The key factors in temperature-dependent cell adhesion/detachment control are discussed from the viewpoint of grafting temperature-responsive polymers, and new methodologies for effective cell sheet culturing and the construction of thick tissues are summarized.

Synthesis of a Kind of Temperature-responsive Cell Culture Surface for Corneal Sheet

2010 ◽  
Vol 26 (12) ◽  
pp. 1119-1126 ◽  
Author(s):  
Yanqing Guan ◽  
Zhibin Li ◽  
Xin Wang ◽  
Xiaoli Ni ◽  
Ami Yang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Temperature Responsive ◽  
Culture Surface ◽  
Responsive Cell
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