Purpose: This research introduces a polymeric nanosphere as a new dispersive solid phase extraction adsorbent for the extraction of methylphenidate from urine and its HPLC analysis. Methods: Polymeric nanosphere is a kind of copolymeric network obtained by copolymerization of an ionic liquid monomer and styrene in the presence of vinyltriethoxysilane and 2-hydroxyethylmethacrylate. High performance liquid chromatography coupled with ultra violet detector was applied for the determination and quantification of methylphenidate. Dominant parameters in extraction were modified by the one-parameter-at-a-time method. The results are as follow: 10 mg of polymeric nanospheres, 400 µL of acetonitrile, 5 mL of urine with the pH value of 9, and the extraction and desorption times of 2 and 5 min, respectively, which can be selected as the optimum extraction conditions. Results: Calibration curve was plotted through optimized conditions, and the proposed method was validated. The results demonstrated that the method presented linearity in the concentration range of 30-1200 ng/mL. Selectivity, matrix effect and metabolites interference effect were investigated and the method presented no obvious interference effect during the analysis run time. Repeatability, LOD and LOQ values of the method can be reported in this section as well. The method showed satisfactory results with 98.8% relative recovery in the analysis of positive urine samples. Conclusion: The findings convinced the applicability of the introduced method for dispersive solid phase extraction and HPLC analysis of the positive urine samples in different laboratories.
Application of Carbonic Nanosheets Based on Urea Precursors as Dispersive Solid Phase Extraction Adsorbent for Extraction of Methamphetamine from Urine Samples