Polymeric Hydrogel Scaffolds: Skin Tissue Engineering and Regeneration

2021 ◽  
Author(s):  
Varuna Naga Venkata Arjun Uppuluri ◽  
Shanmugarajan Thukani Sathanantham ◽  
Sai Krishna Bhimavarapu ◽  
Lokesh Elumalai
Keyword(s):  
Tissue Engineering ◽  
Effective Rate ◽  
The Body ◽  
Skin Tissue ◽  
The Novel ◽  
Hydrogel Scaffolds ◽  
Skin Tissue Engineering ◽  
Polymeric Hydrogel ◽  
Regenerative Approach ◽  
Tissue Defects

Tissue engineering is a novel regenerative approach in the medicinal field that promises the regeneration of damaged tissues. Moreover, tissue engineering involves synthetic and natural biomaterials that facilitate tissue or organ growth outside the body. Not surprisingly, the demand for polymer-based therapeutical approaches in skin tissue defects has increased at an effective rate, despite the pressing clinical need. Among the 3D scaffolds for tissue engineering and regeneration approaches, hydrogel scaffolds have shown significant importance for their use as 3D cross-linked scaffolds in skin tissue regeneration due to their ideal moisture retention property and porosity biocompatibility, biodegradable, and biomimetic characteristics. In this review, we demonstrated the choice of ideal biomaterials to fabricate the novel hydrogel scaffolds for skin tissue engineering. After a short introduction to the bioactive and drug-loaded polymeric hydrogels, the discussion turns to fabrication and characterisation techniques of the polymeric hydrogel scaffolds. In conclusion, we discuss the excellent wound healing potential of stem cell-loaded hydrogels and Nano-based approaches to designing hydrogel scaffolds for skin tissue engineering.

scholarly journals Formulation and Evaluation of 7, 8- Dihydroxy Flavone Loaded Topical Hydrogel Scaffold

2021 ◽  
Vol 12 (1) ◽  
pp. 416-422
Author(s):  
Varuna Naga Venkata Arjun Uppuluri ◽  
Shanmugarajan T S
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cellular Proliferation ◽  
Current Treatment ◽  
Skin Tissue ◽  
Treatment Modalities ◽  
Hydrogel Scaffold ◽  
Skin Tissue Engineering ◽  
Promising Therapeutic Approach ◽  
Tissue Defects

Burnt skin tissue defects pose a significant challenge for physicians to manage owing to the limited skin regeneration potential. Various conventional approaches (i.e. autografts and allografts) have been endorsed for the restoration of tissue defects with inadequate results. However, skin tissue engineering techniques were appeared as an optimistic approach to improve the skin tissue regeneration. Hydrogels offering advantages over the current treatment modalities of the damaged skin tissue were proposed as an advanced and promising therapeutic approach for effective skin tissue regeneration. In general, an ideal hydrogel scaffold used for burnt skin tissue regeneration must show excellent biodegradability, biocompatibility and bioadhesive characteristics. Moreover, among the variety of several hydrogel scaffolds for skin tissue regeneration, topical hydrogels developed from both natural and synthetic polymers are likely to fulfil these above characteristics. In this study Novel 7, 8- Dihydroxy flavone loaded PVA/Agar hydrogel was developed using the facile physical cross-linking technique. Further, the characterization studies confirmed that this hydrogel scaffold possess an ideal porous cross linked structure along with excellent Hemocompatibility, cellular proliferation and controlled drug release. Histopathological staining’s provided further evidence for the reepithelization, and revascularization potential of the developed hydrogel scaffold. Moreover, our research findings revealed that the 7, 8- Dihydroxy flavone loaded PVA/Agar hydrogel is hemocomapatible and capable of burnt skin tissue repair and also possesses significant potential for skin tissue engineering.

scholarly journals Formulation and Evaluation of 7, 8- Dihydroxy Flavone Loaded Topical Hydrogel Scaffold

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 3150-3156
Author(s):  
Varuna Naga Venkata Arjun Uppuluri ◽  
Shanmugarajan T S
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cellular Proliferation ◽  
Current Treatment ◽  
Skin Tissue ◽  
Treatment Modalities ◽  
Hydrogel Scaffold ◽  
Skin Tissue Engineering ◽  
Promising Therapeutic Approach ◽  
Tissue Defects

Burnt skin tissue defects pose a significant challenge for physicians to manage owing to the limited skin regeneration potential. Various conventional approaches (i.e. autografts and allografts) have been endorsed for the restoration of tissue defects with inadequate results. However, skin tissue engineering techniques were appeared as an optimistic approach to improve the skin tissue regeneration. Hydrogels offering advantages over the current treatment modalities of the damaged skin tissue were proposed as an advanced and promising therapeutic approach for effective skin tissue regeneration. In general, an ideal hydrogel scaffold used for burnt skin tissue regeneration must show excellent biodegradability, biocompatibility and bioadhesive characteristics. Moreover, among the variety of several hydrogel scaffolds for skin tissue regeneration, topical hydrogels developed from both natural and synthetic polymers are likely to fulfil these above characteristics. In this study Novel 7, 8- Dihydroxy flavone loaded PVA/Agar hydrogel was developed using the facile physical cross-linking technique. Further, the characterization studies confirmed that this hydrogel scaffold possess an ideal porous cross linked structure along with excellent Hemocompatibility, cellular proliferation and controlled drug release. Histopathological staining’s provided further evidence for the reepithelization, and revascularization potential of the developed hydrogel scaffold. Moreover, our research findings revealed that the 7, 8- Dihydroxy flavone loaded PVA/Agar hydrogel is hemocomapatible and capable of burnt skin tissue repair and also possesses significant potential for skin tissue engineering.

scholarly journals Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering

2021 ◽  
Vol 6 (3) ◽  
pp. 579-588 ◽  
Author(s):  
Ting Su ◽  
Mengying Zhang ◽  
Qiankun Zeng ◽  
Wenhao Pan ◽  
Yijing Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Hydrogel Scaffolds ◽  
Skin Tissue Engineering ◽  
Agarose Hydrogel

scholarly journals Fabrication and characterization of scaffolds containing different amounts of allantoin for skin tissue engineering

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yeganeh Dorri Nokoorani ◽  
Amir Shamloo ◽  
Maedeh Bahadoran ◽  
Hamideh Moravvej
Keyword(s):  
Tissue Engineering ◽  
Cell Viability ◽  
Healing Process ◽  
The Body ◽  
Skin Tissue ◽  
Sem Images ◽  
Average Pore ◽  
Skin Tissue Engineering ◽  
Freeze Dried

AbstractUsing the skin tissue engineering approach is a way to help the body to recover its lost skin in cases that the spontaneous healing process is either impossible or inadequate, such as severe wounds or burns. In the present study, chitosan/gelatin-based scaffolds containing 0.25, 0.5, 0.75, and 1% allantoin were created to improve the wounds’ healing process. EDC and NHS were used to cross-link the samples, which were further freeze-dried. Different in-vitro methods were utilized to characterize the specimens, including SEM imaging, PBS absorption and degradation tests, mechanical experiments, allantoin release profile assessment, antibacterial assay, and cell viability and adhesion tests. The results indicated that the scaffolds’ average pore sizes were approximately in the range of 390–440 µm, and their PBS uptake amounts were about 1000% to 1250% after being soaked in PBS for 24 h. Around 70% of the specimens were degraded in 6 days, but they were not fully degraded after 21 days. Besides, the samples showed antibacterial activity against S. aureus and E. coli bacteria. In general, the MTT cell viability test indicated that the cells’ density increased slightly or remained the same during the experiment. SEM images of cells seeded on the scaffolds indicated appropriate properties of the scaffolds for cell adhesion.

Aloe vera incorporated biomimetic nanofibrous scaffold: a regenerative approach for skin tissue engineering

2014 ◽  
Vol 23 (3) ◽  
pp. 237-248 ◽  
Author(s):  
S. Suganya ◽  
J. Venugopal ◽  
S. Agnes Mary ◽  
S. Ramakrishna ◽  
B. S. Lakshmi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Aloe Vera ◽  
Nanofibrous Scaffold ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Regenerative Approach

Preparation, Characterization and Assessment of the Novel Gelatin–tamarind Gum/Carboxymethyl Tamarind Gum-Based Phase-Separated Films for Skin Tissue Engineering Applications

2016 ◽  
Vol 56 (2) ◽  
pp. 141-152 ◽  
Author(s):  
Gauri Shankar Shaw ◽  
Dibyajyoti Biswal ◽  
Anupriya B ◽  
Indranil Banerjee ◽  
Krishna Pramanik ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
The Novel ◽  
Engineering Applications ◽  
Skin Tissue Engineering

Needleless electrospinning of PVP/PGS fibrous scaffolds for skin tissue engineering applications

2018 ◽  
Author(s):  
Antonios Keirouz ◽  
Giuseppino Fortunato ◽  
Anthony Callanan ◽  
Norbert Radacsi
Keyword(s):  
Tissue Engineering ◽  
Tensile Modulus ◽  
Dermal Fibroblasts ◽  
Skin Tissue ◽  
Skin Substitute ◽  
Electrospinning Technique ◽  
Skin Tissue Engineering ◽  
Needleless Electrospinning ◽  
High Concentrations

Scaffolds and implants used for tissue engineering need to be adapted for their mechanical properties with respect to their environment within the human body. Therefore, a novel composite for skin tissue engineering is presented by use of blends of Poly(vinylpyrrolidone) (PVP) and Poly(glycerol sebacate) (PGS) were fabricated via the needleless electrospinning technique. The formed PGS/PVP blends were morphologically, thermochemically and mechanically characterized. The morphology of the developed fibers related to the concentration of PGS, with high concentrations of PGS merging the fibers together plasticizing the scaffold. The tensile modulus appeared to be affected by the concentration of PGS within the blends, with an apparent decrease in the elastic modulus of the electrospun mats and an exponential increase of the elongation at break. Ultraviolet (UV) crosslinking of PGS/PVP significantly decreased and stabilized the wettability of the formed fiber mats, as indicated by contact angle measurements. In vitro examination showed good viability and proliferation of human dermal fibroblasts over the period of a week. The present findings provide important insights for tuning the elastic properties of electrospun material by incorporating this unique elastomer, as a promising future candidate for skin substitute constructs.

Optimization and evaluation of ciprofloxacin-loaded collagen/chitosan scaffolds for skin tissue engineering

3 Biotech ◽  
2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Satyavrat Tripathi ◽  
Bhisham Narayan Singh ◽  
Divakar Singh ◽  
Gaurav kumar ◽  
Pradeep Srivastava
Keyword(s):  
Tissue Engineering ◽  
Skin Tissue ◽  
Skin Tissue Engineering ◽  
Chitosan Scaffolds
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