Effect of benvitimod on the proliferation of, inflammatory cytokine secretion by and skin barrier factor production by HaCaT cells

2020 ◽  
Keyword(s):  
Inflammatory Cytokine ◽  
Skin Barrier ◽  
Cytokine Secretion ◽  
Hacat Cells ◽  
Factor Production ◽  
Barrier Factor

Inhibition of Pro-Inflammatory Cytokine Secretion by Select Antioxidants in Human Coronary Artery Endothelial Cells

2020 ◽  
Vol 90 (1-2) ◽  
pp. 103-112 ◽  
Author(s):  
Michael J. Haas ◽  
Marilu Jurado-Flores ◽  
Ramadan Hammoud ◽  
Victoria Feng ◽  
Krista Gonzales ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ascorbic Acid ◽  
Coronary Artery ◽  
Inflammatory Cytokines ◽  
Inflammatory Cytokine ◽  
Culture Media ◽  
Cytokine Secretion ◽  
Human Coronary Artery ◽  
Tnf Α ◽  
Pro Inflammatory Cytokines

Abstract. Inflammatory and oxidative stress in endothelial cells are implicated in the pathogenesis of premature atherosclerosis in diabetes. To determine whether high-dextrose concentrations induce the expression of pro-inflammatory cytokines, human coronary artery endothelial cells (HCAEC) were exposed to either 5.5 or 27.5 mM dextrose for 24-hours and interleukin-1β (IL-1β), interleukin-2 (IL-2), interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor α (TNF α) levels were measured by enzyme immunoassays. To determine the effect of antioxidants on inflammatory cytokine secretion, cells were also treated with α-tocopherol, ascorbic acid, and the glutathione peroxidase mimetic ebselen. Only the concentration of IL-1β in culture media from cells exposed to 27.5 mM dextrose increased relative to cells maintained in 5.5 mM dextrose. Treatment with α-tocopherol (10, 100, and 1,000 μM) and ascorbic acid (15, 150, and 1,500 μM) at the same time that the dextrose was added reduced IL-1β, IL-6, and IL-8 levels in culture media from cells maintained at 5.5 mM dextrose but had no effect on IL-1β, IL-6, and IL-8 levels in cells exposed to 27.5 mM dextrose. However, ebselen treatment reduced IL-1β, IL-6, and IL-8 levels in cells maintained in either 5.5 or 27.5 mM dextrose. IL-2 and TNF α concentrations in culture media were below the limit of detection under all experimental conditions studied suggesting that these cells may not synthesize detectable quantities of these cytokines. These results suggest that dextrose at certain concentrations may increase IL-1β levels and that antioxidants have differential effects on suppressing the secretion of pro-inflammatory cytokines in HCAEC.

High Glucose Enhances Neurotoxicity and Inflammatory Cytokine Secretion by Stimulated Human Astrocytes

2017 ◽  
Vol 14 (7) ◽  
Author(s):  
Manpreet Bahniwal ◽  
Jonathan P. Little ◽  
Andis Klegeris
Keyword(s):  
High Glucose ◽  
Inflammatory Cytokine ◽  
Cytokine Secretion ◽  
Human Astrocytes

Nobiletin reduces LPL-mediated lipid accumulation and pro-inflammatory cytokine secretion through upregulation of miR-590 expression

2019 ◽  
Vol 508 (1) ◽  
pp. 97-101 ◽  
Author(s):  
Ping-Ping He ◽  
Qian-Qian Shen ◽  
Min Wen ◽  
Jie-Qiong Zou ◽  
Yan Wang ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Inflammatory Cytokine ◽  
Cytokine Secretion

Endotoxin recognition in fish results in inflammatory cytokine secretion not gene expression

2010 ◽  
Vol 17 (1) ◽  
pp. 16-28 ◽  
Author(s):  
Nerea Roher ◽  
Agnes Callol ◽  
Josep V. Planas ◽  
Frederick W. Goetz ◽  
Simon A. MacKenzie
Keyword(s):  
Gene Expression ◽  
Inflammatory Cytokine ◽  
Cytokine Secretion

Enhancement of lipopolysaccharide-induced toll-like receptor 2 expression and inflammatory cytokine secretion in HUVECs under high glucose conditions

Life Sciences ◽  
2013 ◽  
Vol 92 (10) ◽  
pp. 582-588 ◽  
Author(s):  
Ying-ying Chen ◽  
Jie Chen ◽  
Jian-wei Hu ◽  
Zi-li Yang ◽  
Yue-liang Shen
Keyword(s):  
High Glucose ◽  
Inflammatory Cytokine ◽  
Cytokine Secretion ◽  
Toll Like Receptor ◽  
Toll Like Receptor 2

scholarly journals Eucalyptus citriodora extract regulates cutaneous homeostasis including immune dysregulation and skin barrier dysfunction via the modulation of peroxisome proliferator-activated receptor-delta (PPAR-delata) pathway

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Akihiro Aioi ◽  
Takuhiro Yamada
Keyword(s):  
Mrna Expression ◽  
Skin Barrier ◽  
Immune Dysregulation ◽  
Keratinocyte Differentiation ◽  
Hacat Cells ◽  
Skin Disorders ◽  
Eucalyptus Citriodora ◽  
Barrier Dysfunction ◽  
Differentiation Markers ◽  
Transfected Cells

Perturbation of cutaneous homeostasis including immune dysregulation and skin barrier dysfunction evokes skin disorders. In this study, we examined the effect of Eucalyptus citriodora (Euc-c) extract on cytokine production, cell proliferation and cell differentiation in HaCaT cells to elucidate its influence on cutaneous homeostasis. Euc-c suppressed significantly LPS-induced IL-6 and TNF-a-induced IL-8 production from HaCaT cells. Conversely IL-1ra production was significantly enhanced by Euc-c. The expressions of IVL, CERS3 and CERS4, keratinocyte differentiation markers, were upregulated to 3.1, 2.8 and 2.7-fold respectively by Euc-c treatment, compared to the control, while the proliferation was downregulated. The lipid contents in Euc-c-treated cells tended to increase, compared with non-treated cells. To explore the underlying mechanism of these effect, we next performed siRNA experiments against PPAR-b/d. Euc-c enhanced PPAR-b/d mRNA expression to 3.25-fold, while PPAR-b/d mRNA expression in transfected cells was suppressed. The expressions of IVL, CERS3 and CERS4 in transfected cells were suppressed to 1.48, 0.82 and 0.72-fold respectively, concomitant with suppression of PPAR-b/d mRNA expression. These results indicated that Euc-c exerts anti-inflammatory effects and regulates keratinocyte differentiation via the modulation of PPAR-b/d pathway. Therefore, the application of Euc-c is expected to exert beneficial effect on skin disorders evoked by perturbation of skin homeostasis.Key words: Eucalyptus citriodora, PPAR-b/d, inflammation, barrier function, cutaneous homeostasis

Mast cell-lung fibroblast interactions signal inflammatory cytokine secretion†

2002 ◽  
Vol 109 (1) ◽  
pp. S22-S22
Author(s):  
S Matthew Fitzgerald ◽  
Steven A Lee ◽  
Kenton Hall ◽  
Kai Liu ◽  
David Chi ◽  
...  
Keyword(s):  
Mast Cell ◽  
Inflammatory Cytokine ◽  
Lung Fibroblast ◽  
Cytokine Secretion

scholarly journals Cytotoxicity studies of Fe 3 O 4 nanoparticles in chicken macrophage cells

2020 ◽  
Vol 7 (4) ◽  
pp. 191561 ◽  
Author(s):  
Shan Zhang ◽  
Shu Wu ◽  
Yiru Shen ◽  
Yunqi Xiao ◽  
Lizeng Gao ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Cell Activity ◽  
Cytokine Secretion ◽  
Ros Production ◽  
Cytotoxic Effects ◽  
Macrophage Cells ◽  
Cytotoxicity Studies ◽  
Significant Difference ◽  
Chicken Macrophage ◽  
Different Doses

Magnetic Fe 3 O 4 nanoparticles (Fe 3 O 4 -NPs) have been widely investigated for their biomedical applications. The main purpose of this study was to evaluate the cytotoxic effects of different sizes of Fe 3 O 4 -NPs in chicken macrophage cells (HD11). Experimental groups based on three sizes of Fe 3 O 4 -NPs (60, 120 and 250 nm) were created, and the Fe 3 O 4 -NPs were added to the cells at different doses according to the experimental group. The cell activity, oxidative index (malondialdehyde (MDA), superoxide dismutase (SOD) and reactive oxygen species (ROS)), apoptosis and pro-inflammatory cytokine secretion level were detected to analyse the cytotoxic effects of Fe 3 O 4 -NPs of different sizes in HD11 cells. The results revealed that the cell viability of the 60 nm Fe 3 O 4 -NPs group was lower than those of the 120 and 250 nm groups when the same concentration of Fe 3 O 4 -NPs was added. No significant difference in MDA was observed among the three Fe 3 O 4 -NP groups. The SOD level and ROS production of the 60 nm group were significantly greater than those of the 120 and 250 nm groups. Furthermore, the highest levels of apoptosis and pro-inflammatory cytokine secretion were caused by the 60 nm Fe 3 O 4 -NPs. In conclusion, the smaller Fe 3 O 4 -NPs produced stronger cytotoxicity in chicken macrophage cells, and the cytotoxic effects may be related to the oxidative stress and apoptosis induced by increased ROS production as well as the increased expression of pro-inflammatory cytokines.

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