scholarly journals Deteksi transmisi transovarial virus dengue pada Aedes aegypti dengan teknik imunositokimia di Kota Manado

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Lidiasani P. Mosesa ◽  
Angle Sorisi ◽  
Victor D. Pijoh
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Vertical Transmission ◽  
Horizontal Transmission ◽  
Research Result ◽  
Transovarial Transmission ◽  
Aedes Mosquito ◽  
Common Occurrence ◽  
Urban Village ◽  
Result Show

Abstract: Capability of Dengue virus to maintain its existence by two mechanism, that is horizontal transmission between veremia vertebrata that contracted by Aedes mosquito and vertical transmission from female mosquito infective to next generation. The most mechanism common occurrence is vertical transmission whereabout of transovarial transmission can be detected by many kind of way, one of way is immunositochemical technic. Immunositochemical is reported to be able detect antigen Dengue virus in low level. This research aims to prove whereabout of transovarial transmission Dengue virus on Aedes aegypti in Manado city. Researche sampel is Aedes aegypti mosquitos from eggs Aedes aegypti that was taken from urban village that is DBD case. This research was held on November 2015 – January 2016. Research result show that Aedes aegypti mosquito antigen positive Dengue are 24 tail of mosquitos from 48 tail of mosquitos that was tested with Index Transmissi Transovarial (ITT) about 39,1% - 70%. Based on research result can be proven that there is transovarial transmission Dengue virus on Aedes aegypti in West Kombos Urban Village, Singkil Distric, Manado City.Keywords: transovarial transmission, dengue virus, aedes aegypti, immunositochemical technicAbstrak: Kemampuan Virus Dengue untuk mempertahankan keberadaannya melalui dua mekanisme yaitu transmisi horizontal antara vertebrata viremia yang ditularkan oleh nyamuk Aedes dan transmisi vertikal (transovarial) yaitu dari nyamuk betina infektif ke generasi berikutnya. Mekanisme yang paling umum terjadi adalah transmisi vertikal (transovarial). Ada tidaknya transmisi transovarial dapat diketahui dengan berbagai cara, salah satunya dengan teknik imunositokimia. Metode imunositokimia dilaporkan dapat mendeteksi antigen virus Dengue dalam kadar yang rendah. Penelitian ini bertujuan untuk membuktikan ada tidaknya transmisi transovarial virus Dengue pada Aedes aegypti di kota Manado. Sampel penelitian adalah nyamuk Aedes aegypti yang berasal dari telur Aedes yang diambil dari kelurahan yang terdapat kasus DBD. Penelitian ini dilaksanakan pada bulan November 2015 – Januari 2016. Hasil penelitian menunjukkan bahwa nyamuk Aedes aegypti yang positif antigen virus Dengue berjumlah 24 ekor nyamuk dari 48 ekor nyamuk yang diperiksa dengan Index Transmisi Transovarial (ITT) berkisar 39,1% - 70%. Berdasarkan hasil penelitian tersebut, terbukti bahwa adanya transmisi transovarial virus Dengue pada Aedes aegypti di Kelurahan Kombos Barat Kecamatan Singkil, Kota Manado.Kata kunci: transmisi transovarial, virus dengue, aedes aegypti, teknik imunositokimia

scholarly journals TRANSMISI TRANSOVARIAL VIRUS DENGUE PADA NYAMUKAEDES SPP.

2013 ◽  
Vol 5 (1) ◽  
Author(s):  
Angle M. H. Sorisi
Keyword(s):  
Dengue Virus ◽  
Vertical Transmission ◽  
Dengue Hemorrhagic Fever ◽  
Virus Transmission ◽  
Hemorrhagic Fever ◽  
Health Problems ◽  
Transovarial Transmission ◽  
Blood Sucking ◽  
Endemic Areas ◽  
And Control

Abstract: Dengue Hemorrhagic Fever (DHF) is one of the most serious health problems in Indonesia which often causes outbreaks with numerous deaths. The disease is transmitted byAedes sp.females. Generally, dengue virus transmission occurs horizontally from human carriers, and the dengue viruses are passed on bytheir vectors through blood sucking activity. After propagation in the mosquito, the viruses are transmitted to human recipients. In addition, there is a vertical transmission (transovarial) of dengue virusesin the ova of Aedes sp.females. The viruses propagate in the ova that undergo  metamorphosis to become larvae, pupae, and imagoes. The transovarial transmission of dengue virusesin its vectors in endemic areas could be a causative key which is responsible for the phenomenon of increasing cases of DHF. Any effort to prevent and control DHF requires a thorough understanding about virDen transmission, including this transovarial transmission in Aedes spfemales. Keywords: DHF, transovarial transmission, Aedes sp.     Abstrak: Penyakit Demam Berdarah Dengue (DBD) merupakan salah satu masalah kesehatan yang semakin serius di Indonesia dan sering menimbulkan suatu Kejadian Luar Biasa (KLB) dengan jumlah kematian tinggi. Penyakit ditularkan melalui Aedes sp.betina. Transmisi virus dengue umumnya terjadi secara horizontal, yaitu dari manusia pembawa virus dengue ke nyamuk vektor Aedes sp. melalui aktivitasnya mengisap darahSetelah mengalami propagasi  dalam  tubuh nyamuk, virus dengue ditularkan ke  manusia penerima. Selain itu, transmisi virus dapat terjadi secara vertikal (transovarial) yaitu virus dengue dalam tubuh nyamuk vektorAedes sp. betinake ovum, kemudian berpropagasi dalam ovum, larva, pupa, dan imago. Transmisi transovarial virus dengueke vektornya di daerah endemik bisa menjadi kunci penyebab yang bertanggung jawab terhadap fenomena peningkatan kasus deman berdarah dengue. Upaya pencegahan dan penanggulangan DBD memerlukan pengetahuan yang matang tentang adanya infeksi transovarial virDen pada nyamuk Aedes sp. Kata kunci : DBD, transmisi transovarial,  Aedes sp.

scholarly journals Vertical Transmission of Dengue Virus in Aedes aegypti Collected in Surabaya, Indonesia, during 2008^|^ndash;2011

2012 ◽  
Vol 65 (3) ◽  
pp. 274-276 ◽  
Author(s):  
Kris Cahyo Mulyatno ◽  
Atsushi Yamanaka ◽  
Subagyo Yotopranoto ◽  
Eiji Konishi
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Vertical Transmission

scholarly journals Natural transovarial transmission of dengue virus 4 in Aedes aegypti from Cuiabá, State of Mato Grosso, Brazil

2015 ◽  
Vol 48 (1) ◽  
pp. 18-25 ◽  
Author(s):  
Lucinéia Claudia de Toni Aquino da Cruz ◽  
Otacília Pereira Serra ◽  
Fábio Alexandre Leal-Santos ◽  
Ana Lucia Maria Ribeiro ◽  
Renata Dezengrini Slhessarenko ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Transovarial Transmission ◽  
Mato Grosso

scholarly journals VERTICAL TRANSMISSION OF DENGUE VIRUS IN Aedes aegypti COLLECTED IN PUERTO IGUAZÚ, MISIONES, ARGENTINA

2014 ◽  
Vol 56 (2) ◽  
pp. 165-167 ◽  
Author(s):  
Manuel Espinosa ◽  
Sergio Giamperetti ◽  
Marcelo Abril ◽  
Alfredo Seijo
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Vertical Transmission ◽  
Winter Period ◽  
Rt Pcr ◽  
Epidemiological Situation

A finding of vertical transmission of the DEN 3 virus in male specimens of Aedes aegypti, collected in the 2009 fall-winter period, in Puerto Iguazú city, Misiones, Argentina, using the RT-PCR technique in a 15-specimen pool is reported. This result is analyzed within the context of the epidemiological situation of Argentina's northeast border.

scholarly journals Highly Efficient Vertical Transmission for Zika Virus in Aedes aegypti after Long Extrinsic Incubation Time

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 366 ◽  
Author(s):  
Menchie Manuel ◽  
Dorothée Missé ◽  
Julien Pompon
Keyword(s):  
Viral Load ◽  
Aedes Aegypti ◽  
Vertical Transmission ◽  
Incubation Time ◽  
Zika Virus ◽  
Horizontal Transmission ◽  
Oral Infection ◽  
Gonotrophic Cycle ◽  
Pooled Samples ◽  
Shed Light

While the Zika virus (ZIKV) 2014–2017 pandemic has subsided, there remains active transmission. Apart from horizontal transmission to humans, the main vector Aedes aegypti can transmit the virus vertically from mother to offspring. Large variation in vertical transmission (VT) efficiency between studies indicates the influence of parameters, which remain to be characterized. To determine the roles of extrinsic incubation time and gonotrophic cycle, we deployed an experimental design that quantifies ZIKV in individual progeny and larvae. We observed an early infection of ovaries that exponentially progressed. We quantified VT rate, filial infection rate, and viral load per infected larvae at 10 days post oral infection (d.p.i.) on the second gonotrophic cycle and at 17 d.p.i. on the second and third gonotrophic cycle. As compared to previous reports that studied pooled samples, we detected a relatively high VT efficiency from 1.79% at 10 d.p.i. and second gonotrophic cycle to 66% at 17 d.p.i. and second gonotrophic cycle. At 17 d.p.i., viral load largely varied and averaged around 800 genomic RNA (gRNA) copies. Longer incubation time and fewer gonotrophic cycles promoted VT. These results shed light on the mechanism of VT, how environmental conditions favor VT, and whether VT can maintain ZIKV circulation.

scholarly journals An Updated Review of the Invasive Aedes Albopictus in the Americas; Geographical Distribution, Host Feeding Patterns, Arbovirus Infection, and the Potential for Vertical Transmission of Dengue Virus

2021 ◽  
Author(s):  
Julian E. Garcia-Rejon ◽  
Juan-Carlos Navarro ◽  
Nohemi Cigarroa-Toledo ◽  
Carlos M. Baak-Baak
Keyword(s):  
Dengue Virus ◽  
Vertical Transmission ◽  
Natural Infection ◽  
Horizontal Transmission ◽  
Blood Feeding ◽  
The United States ◽  
Host Feeding ◽  
Feeding Patterns ◽  
Cache Valley Virus ◽  
Dengue Disease

Aedes (Stegomyia) albopictus is a mosquito native to Southeast Asia. Currently, it has a wide distribution in America where natural infection with arboviruses of medical and veterinary importance has been reported. In spite of their importance in the transmission of endemic arbovirus, basic information of parameters affecting their vectorial capacity is poorly investigated. The aim of the work was to update the distribution range of Ae. albopictus in the Americas, review the blood-feeding patterns and compare the minimum infection rate (MIR) of the dengue virus (DENV) between studies of vertical and horizontal transmission. The current distribution of Ae. albopictus encompasses 21 countries in the Americas. Extensive review has been conducted for the blood-feeding patterns of Ae. albopictus. The results suggest that the mosquito is capable of feeding on 16 species of mammals and five species of avian. Humans, dogs, and rats are the most common host. Eight arboviruses with the potential to infect humans and animals have been isolated in Ae. albopictus. In the United States of America (USA), Eastern equine encephalitis virus, Keystone virus, La Crosse Virus, West Nile virus, and Cache Valley virus were isolated in the Asian mosquito. In Brazil, Mexico, Colombia, and Costa Rica, DENV (all serotypes) has been frequently identified in field-caught Ae. albopictus. Overall, the estimated MIR in Ae. albopictus infected with DENV is similar between horizontal (10.95) and vertical transmission (8.28). However, in vertical transmission, there is a difference in the MIR values if the DENV is identified from larvae or adults (males and females emerged from a collection of eggs or larvae). MIR estimated from larvae is 14.04 and in adults is 4.04. In conclusion, it has to be highlighted that Ae. albopictus is an invasive mosquito with wide phenotypic plasticity to adapt to broad and new areas, it is highly efficient to transmit the DENV horizontally and vertically, it can participate in the inter-endemic transmission of the dengue disease, and it can spread zoonotic arboviruses across forest and urban settings.

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