Organophosphate insecticide susceptible test and transovarial transmission detection of dengue virus on Aedes aegypti in Kendari

Abstract: Capability of Dengue virus to maintain its existence by two mechanism, that is horizontal transmission between veremia vertebrata that contracted by Aedes mosquito and vertical transmission from female mosquito infective to next generation. The most mechanism common occurrence is vertical transmission whereabout of transovarial transmission can be detected by many kind of way, one of way is immunositochemical technic. Immunositochemical is reported to be able detect antigen Dengue virus in low level. This research aims to prove whereabout of transovarial transmission Dengue virus on Aedes aegypti in Manado city. Researche sampel is Aedes aegypti mosquitos from eggs Aedes aegypti that was taken from urban village that is DBD case. This research was held on November 2015 – January 2016. Research result show that Aedes aegypti mosquito antigen positive Dengue are 24 tail of mosquitos from 48 tail of mosquitos that was tested with Index Transmissi Transovarial (ITT) about 39,1% - 70%. Based on research result can be proven that there is transovarial transmission Dengue virus on Aedes aegypti in West Kombos Urban Village, Singkil Distric, Manado City.Keywords: transovarial transmission, dengue virus, aedes aegypti, immunositochemical technicAbstrak: Kemampuan Virus Dengue untuk mempertahankan keberadaannya melalui dua mekanisme yaitu transmisi horizontal antara vertebrata viremia yang ditularkan oleh nyamuk Aedes dan transmisi vertikal (transovarial) yaitu dari nyamuk betina infektif ke generasi berikutnya. Mekanisme yang paling umum terjadi adalah transmisi vertikal (transovarial). Ada tidaknya transmisi transovarial dapat diketahui dengan berbagai cara, salah satunya dengan teknik imunositokimia. Metode imunositokimia dilaporkan dapat mendeteksi antigen virus Dengue dalam kadar yang rendah. Penelitian ini bertujuan untuk membuktikan ada tidaknya transmisi transovarial virus Dengue pada Aedes aegypti di kota Manado. Sampel penelitian adalah nyamuk Aedes aegypti yang berasal dari telur Aedes yang diambil dari kelurahan yang terdapat kasus DBD. Penelitian ini dilaksanakan pada bulan November 2015 – Januari 2016. Hasil penelitian menunjukkan bahwa nyamuk Aedes aegypti yang positif antigen virus Dengue berjumlah 24 ekor nyamuk dari 48 ekor nyamuk yang diperiksa dengan Index Transmisi Transovarial (ITT) berkisar 39,1% - 70%. Berdasarkan hasil penelitian tersebut, terbukti bahwa adanya transmisi transovarial virus Dengue pada Aedes aegypti di Kelurahan Kombos Barat Kecamatan Singkil, Kota Manado.Kata kunci: transmisi transovarial, virus dengue, aedes aegypti, teknik imunositokimia

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Transovarial transmission of Dengue virus 1 and 2 as showed by detection in Aedes aegypti larvae

VIRUS Reviews & Research ◽

10.17525/vrrjournal.v9i2.231 ◽

2004 ◽

Vol 9 (2) ◽

Cited By ~ 1

Author(s):

Alzira B. Cecilio ◽

Marcelo De C. Resende ◽

Fernanda C. M. Jorge ◽

Simone M. De Resende ◽

Mariana G. De Brito ◽

...

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Transovarial Transmission

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Deteksi Transmisi Transovarial Virus Dengue PadaAedes AegyptiPada Wilayah Endemis Kota Pontianak

Prosiding Seminar Nasional Kesehatan ◽

10.48144/prosiding.v1i.779 ◽

2021 ◽

Vol 1 ◽

pp. 984-995

Author(s):

Malik Saepudin ◽

Heru Subaris Kasjono ◽

M Martini

Keyword(s):

Polymerase Chain Reaction ◽

Aedes Aegypti ◽

Dengue Virus ◽

Aedes Albopictus ◽

Transovarial Transmission ◽

Chain Reaction ◽

Cross Sectional ◽

Transmission Index ◽

Virus Serotype ◽

Polymerase Chain

AbstractDengue virus is highly pathogenic in humans and spreads rapidly through Aedes aegypti and Aedes albopictus mosquitoes. More than half a billion people from 100 countries in the world are at serious risk of dengue virus infection. The purpose of this study was to prove the existence of transovarial transmission of dengue virus in Aedes aegypti mosquitoes with a transovarial transmission index (TTI) in endemic areas in Pontianak, West Kalimantan. This research method is descriptive observational with a cross-sectional study. The results of the Microscopic Examination of Head Squash Preparations on the Aedes aegypti mosquito, showed the Transovarial Transmission Index in Batulayang Village was 39.60% higher, compared to Sungai Jawi Village, which was 29.30%, but both were still lower than ITT in 2012. The results of the Aedes mosquito examination aegypti using the Polymerase Chain Reaction Transcription Reaction (PCR-TR) method found the dengue virus strain. The conclusion of this study proves that the transovarial transmission of dengue virus in Aedes aegypti mosquitoes in Sungai Jawi Village is 29.30% lower than in Batulayang Village by 39.60%, and the dengue virus serotype, DENV-3, has been found.Keywords: transovarial transmission; dengue virus; Aedes aegypti AbstrakVirus Dengue sangat patogen pada manusia dan menyebar dengan cepat melalui nyamuk Aedes aegypti dan Aedes albopictus. Lebih dari setengah miliar penduduk dari 100 negara di dunia berada pada risiko serius infeksi virus dengue. Tujuan dari penelitian ini adalah untuk membuktikan adanya penularan virus dengue transovarial pada nyamuk Aedes aegypti dengan indeks transmisi transovarial (TTI) di daerah Endemis di Pontianak, Kalimantan Barat. Metode penelitian ini adalah deskriptif observasional dengan studi potong lintang. Hasil Pemeriksaan Mikroskopis Sediaan Head Squash pada nyamuk Aedes aegypti, menunjukan Indeks Transmisi Transovarial pada Kelurahan Batulayang lebih tinggi yaitu 39,60%, dibandingkan dengan Kelurahan Sungai Jawi yaitu 29,30%, tetapi keduanya masih lebih rendah dibandingkan ITT pada Tahun 2012. Hasil pemeriksaan nyamuk Aedes aegypti dengan metode Polymerase Chain Reaction Transcription Reaction(PCR-TR) ditemukan strain virus Dengue-3. Kesimpulan penelitian ini membuktikan adanya transmisi transovarial virus dengue pada nyamuk Aedes aegypti di Kelurahan Sungai Jawi sebesar 29,30% lebih rendah dibandingkan di kelurahan Batulayang sebesar 39,60%, serta berhasil ditemukan serotipe virus Dengue yaitu DENV-3.Kata Kunci : transmisi transovarial; virus dengue; Aedes aegypti

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PENYEBARAN VIRUS DENGUE SECARA TRANSOVARIAL PADA VEKTOR DEMAM BERDARAH DENGUE NYAMUK Aedes aegypti

HOLISTIK JURNAL KESEHATAN ◽

10.33024/hjk.v12i4.81 ◽

2019 ◽

Vol 12 (4) ◽

pp. 216-223

Author(s):

Devita Febriani Putri ◽

Nurhaida Widiani ◽

Debi Arivo

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Prediction Models ◽

Virus Transmission ◽

Hemorrhagic Fever ◽

Transovarial Transmission ◽

Scientific Article ◽

Vector Surveillance ◽

Population Vector ◽

Dengue Outbreaks

TRANSOVARIAL TRANSMISSION OF DENV IN AEDES AEGYPTI Background: Transovarial transmission of dengue virus in Aedes aegypti mosquitoes is a vertical transmission of dengue virus infection in female Ae. aegypti mosquitoes to the offspring. The phenomenon of transovarial dengue virus transmission in Dengue Hemorrhagic Fever (DHF) vectors has been proven by laboratory and nature, which indicates the transovarial transmission of dengue virus has an important role in maintaining the dengue epidemic. DHF vector control especially Ae. aegypti mosquitoes is an effective method of stopping transmission and expansion of dengue cases.Purpose: This scientific article aims to understand the spread of dengue virus transovarially in dengue mosquito vectors, and its relation to the prediction of outbreak dengue cases as information on DHF vector surveillance so that it can make the appropriate control program.Methods: Collecting several scientific articles to obtain information on the studies that have been done and summarizing the results of the study.Results: Several result of study are proving that transovarial transmission of dengue virus in Aedes spp. mosquitoes can predict dengue outbreaks case by monitoring the stadium immature Aedes sp., but it need further comprehension statistically about occurrence of dengue outbreaks and the increasing of dengue virus infections in immature stadium of mosquitoes.Discussion: Transovarial transmission rates from Ae. aegypti mosquito sample obtained from nature may be lower than in the laboratory, because laboratory condition can be controlled in accordande with the development of viruses in mosquito bodies. The dengue virus is proven to be able to spread between stages from eggs, larvae, pupae to imago and Ae. aegypti mosquitoes can act as reservoirs for dengue virus until the 7th progeni.Conclusion: Dispersion dengue virus through transovarial in Ae. aegypti mosquito playing important role in viruses maintained in nature during absence of viremic vertebrata host or when the climate condition are not favorable for that viruses. Continuous monitoring of Ae. aegypti mosquitoes population vector related the early detection of virus circulation may contribute to the prediction models for dengue outbreaks, so that DHF control can be more effective.Pendahuluan: Penularan virus dengue secara transovarial pada nyamuk Aedes aegypti adalah transmisi secara vertikal dari nyamuk Ae. aegypti betina yang infektif virus dengue kepada keturunannya. Fenomena penularan transovarial virus dengue pada vektor Demam Berdarah Dengue (DBD) telah banyak dibuktikan skala laboratorium dan secara natural di alam, yang mengindikasikan penularan transovarial virus dengue memiliki peranan penting dalam mempertahankan epidemik DBD. Pengendalian vektor DBD khususnya nyamuk Ae. aegypti merupakan salah satu metode efektif dalam menghentikan penularan dan perluasan kasus DBD.Tujuan: Studi ini untuk memahami penyebaran virus dengue secara transovarial pada vektor nyamuk DBD, serta kaitannya terhadap prediksi kasus luar biasa (KLB) DBD sebagai informasi surveilans vektor DBD sehingga dapat membuat program pengendalian yang tepat.Metode: Dengan mengumpulkan beberapa artikel ilmiah untuk mendapatkan informasi studi yang telah dilakukan sebelumnya dan membuat ringkasan dari hasil studi tersebut.Hasil: Beberapa studi membuktikan penelitian transovarial virus dengue pada nyamuk Aedes spp. dapat memprediksi kasus KLB DBD dengan cara memonitoring stadium immature Aedes sp., namun perlu dilakukan studi statistik lebih lanjut untuk membuktikan hubungan terjadinya KLB DBD dan meningkatnya infeksi virus dengue pada stadium immature nyamuk.Pembahasan: Angka infeksi penularan transovarial virus dengue dari sampel nyamuk Ae. aegypti yang didapatkan langsung dari alam lebih rendah dari skala laboratorium, dikarenakan kondisi laboratorium dapat dikendalikan sesuai dengan perkembangan virus ditubuh nyamuk. Virus dengue terbukti dapat menyebar antar stadium dari telur, larva, pupa, sampai imago dan nyamuk Ae. aegypti dapat menjadi reservoir virus dengue sampai progeni ke 7.Kesimpulan: Penyebaran virus dengue secara transovarial pada nyamuk Ae. aegypti berperan penting dalam mempertahankan keberadaan virus di alam khususnya dimana tidak ada hospes vertebrata yang viremik atau ketika keadaan (kondisi iklim) yang tidak menguntungkan virus tersebut di alam. Pemantauan berkelanjutan pada vektor demam berdarah nyamuk Ae. aegypti terkait deteksi dini sirkulasi virus dengue dapat berkontribusi pada pengembangan model prediksi KLB DBD, sehingga pengendalian DBD dapat lebih efektif.

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Transovarial Transmission of Dengue Virus in Aedes aegypti: A Case in Quezon City, Philippine

Acta Medica Philippina ◽

10.47895/amp.v48i4.1051 ◽

2014 ◽

Vol 48 (4) ◽

Author(s):

Ralph Julius G. Bawalan ◽

Nelia P. Salazar ◽

Francisco M. Heralde III

Keyword(s):

Aedes Aegypti ◽

Dengue Virus ◽

Transovarial Transmission ◽

Quezon City

...

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Detection of dengue virus in Aedes mosquitoes in Delhi, India

ENTOMON ◽

10.33307/entomon.v44i3.466 ◽

2019 ◽

Vol 44 (3) ◽

pp. 213-218

Author(s):

Suresh Chand Kaushik ◽

Sukhvir Singh ◽

Purnima Srivastava ◽

R. Rajendran

Keyword(s):

Early Intervention ◽

Early Detection ◽

Aedes Aegypti ◽

Dengue Virus ◽

Infection Rate ◽

Transmission Season ◽

Aedes Mosquitoes ◽

Alternative Approach ◽

Human Sera ◽

Minimum Infection Rate

Detection of viruses in human sera particularly in endemic areas is cumbersome and laborious. Therefore, an alternative approach, Immuno-fluorescence assay (IFA) was performed to determine dengue virus (DENV) positivity in mosquitoes. A total of 1055 adult Aedes aegypti female mosquitoes were tested for IFA test against DENV. Minimum infection rate (MIR) for DENV was found higher during August to November 2016 ranging from 10.75 to 20.83. The average yearly MIR was about 6.64. Higher MIR for Ae. aegypti was found in Sarfabad, Noida (12.71) and Khoda Colony, Ghaziabad (11.90). Minimum MIR (4.67) was observed in Sanjay colony (Faridabad). The main contribution of this study resides in the development of a more suitable monitoring system for early detection of viral circulation and to prioritize early intervention in the non-transmission season.

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Difusi dan Pola Spasial Sebaran Penyakit Demam Berdarah Dengue (DBD) Di Kota Bandar Lampung

KESMARS Jurnal Kesehatan Masyarakat Manajemen dan Administrasi Rumah Sakit ◽

10.31539/kesmars.v1i1.192 ◽

2018 ◽

Vol 1 (1) ◽

pp. 87-95

Author(s):

Nurul Qamila ◽

Agel Vidian Krama

Keyword(s):

Public Health ◽

Spatial Analysis ◽

Aedes Aegypti ◽

Dengue Virus ◽

Spatial Pattern ◽

Dengue Hemorrhagic Fever ◽

Health Problem ◽

Public Health Problem ◽

Hemorrhagic Fever ◽

Descriptive Method

Dengue hemorrhagic fever (DHF) is a contagious disease caused by the dengue virus and is transmitted by the mosquito Aedes aegypti (Aa.aegypti). The population is still a public health problem that increases the number of sufferers and also widespread, with population and education. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the spatial pattern and the spread of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. This study aims to reveal the spatial pattern and distribution of Dengue Hemorrhagic Fever (DHF) with the descriptive method and spatial pattern of Dengue Hemorrhagic Fever (DHF) can result in different locations of these allegations. From the map that can be used for the prevention of Dengue Hemorrhagic Fever (DBD) in Bandar Lampung City. Keywords: DHF, Spatial Analysis

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