scholarly journals IDENTIFIKASI PENANDA ISOZIM PER-7, PER-8, DAN RAPD OB17375 PADA KELAPA GENJAH SALAK (GSK) DAN BEBERAPA HASIL SILANGANNYA DENGAN KELAPA DALAM

EUGENIA ◽  
2016 ◽  
Vol 11 (1) ◽  
Author(s):  
Semuel D. Runtunuwu ◽  
Eddy F. Lengkong
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Rapd Marker ◽  
Lesion Size ◽  
West African ◽  
Starch Gel Electrophoresis ◽  
Chain Reaction ◽  
Isozyme Markers ◽  
Starch Gel ◽  
Polymerase Chain

ABSTRACT Runtunuwu, S.D. and E.F. Lengkong. 2005. Identification of Isozyme Markers (PER-7, PER-8, and RAPD OBI7375) of Dwarf Salak Coconut (GSK) and its Tall Hibrids. Eugenia 11 (1): 8-17. The objective of this research was to identify PER-7 and PER-8 isozymes, and OB17 375 RAPD as molecular markers for resistance to NF Phytophthora disease in Genjah Salak (GSK) dwarf coconut and its several hybrids with Tall coconut at The Research Institute for Coconut and Palmae (RICP) Manado. Coconut resistance to NF Phytophthora disease was determined based on disease lesion size of inoculated coconut fruits at 7 days after inoculation. PER-7 and PER-8 isozymes marker were identified using horizontal starch gel electrophoresis, and OB17375 RAPD marker was identified based on polymerase chain reaction (PCR) using kit B number 17 primer (Operon Technologies, California). PER-7 isozyme marker could be used to select GSK x DTA (Dalam Tenga) and GSK x RLT (Rennell Tall) hybrid coconuts that resistant to NF Phytophthora disease. PER-7 isozyme without OB17375 markers (PER-7/-) could be used to select resistant GSK x WAT (West African Tall) hybrid coconut to the disease. Keywords: Isozymes, RAPD, Molecular marker, Phytophthora disease, coconut

scholarly journals Polymerase Chain Reaction-And Rna Hybridization-Based Method for the Investigation of Deep-Seated Candidiasis

1997 ◽  
Vol 8 (6) ◽  
pp. 329-334 ◽  
Author(s):  
Peter R Couroux ◽  
Zafar Hussain ◽  
Frank Rutledge ◽  
Robert Lannigan ◽  
Edward D Ralph ◽  
...  
Keyword(s):  
Risk Factors ◽  
Polymerase Chain Reaction ◽  
Invasive Candidiasis ◽  
Gel Electrophoresis ◽  
Tertiary Care ◽  
Immunosuppressive Drugs ◽  
Blood Cultures ◽  
Hybridization Method ◽  
Chain Reaction ◽  
Polymerase Chain

OBJECTIVE: To determine the usefulness of a polymerase chain reaction (PCR) and RNA hybridization method for the diagnosis of invasive candidiasis and to compare its sensitivity with blood cultures.DESIGN: Blood cultures and a blood sample for PCR were taken from patients with suspected invasive candidiasis. A 105 base pair conserved segment within the rDNA ofCandidaspecies was amplified. The amplicon was detected by hybridization and gel electrophoresis.SETTING: Intensive care units of two tertiary care hospitals.PATIENTS: One hundred and eighteen patients 16 years of age or older with four more risk factors for invasive candidiasis were enrolled. Present or recent past treatment with broad spectrum antibiotics, cancer chemotherapy, immunosuppressive drugs, granulocytopenia or granulocytosis, intravascular catheterization, tracheal intubation, recent abdominal surgery and parenteral nutrition were considered risk factors.RESULTS: Forty-three patients had invasive candidiasis. PCR detected infections in 28 and 26 patients (sensitivity 65.1% and 60.4%) by hybridization and gel electrophoresis, respectively. The sensitivity of blood cultures was 58.1%. Of 25 patients with positive blood cultures, 17 were positive by PCR with the hybridization method. Eleven patients with invasive candidiasis had negative blood cultures but were positive by PCR.CONCLUSION: PCR, especially with a hybridization detection method, is more sensitive than blood culture for invasive candidiasis and may facilitate the diagnosis of nonfungemic disease.

scholarly journals Epidemiological Investigation ofSalmonella Tileneby Pulsed-Field Gel Electrophoresis and Polymerase Chain Reaction

1997 ◽  
Vol 8 (6) ◽  
pp. 318-322 ◽  
Author(s):  
Chandar M Anand ◽  
Kevin Fonseca ◽  
Ken Longmore ◽  
Robert Rennie ◽  
Linda Chui ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gel Electrophoresis ◽  
Washington State ◽  
Pulsed Field Gel Electrophoresis ◽  
Dna Fingerprint ◽  
Rrna Genes ◽  
23S Rrna ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Polymerase Chain

Pulsed-field gel electrophoresis (PFGE) and DNA fingerprinting by the polymerase chain reaction (PCR) were performed on 11 isolates ofSalmonella tilene. Five strains were from a cluster of human patients, six from sugar gliders and pygmy hedgehogs kept as family pets or from local pet retailers, and one isolate from the first North American case ofS tilenedescribed in Washington State in 1994. The PFGE restriction patterns showed all isolates to be similar. However, PCR using primers to the 16S and 23S rRNA genes ofEscherichia colidemonstrated that the Washington State isolate differed from the rest of the other isolates, which were all similar based upon their DNA fingerprint. This study indicates that reliance on one technique alone may be insufficient to show nuances between strains that are, in many respects, closely related.

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