scholarly journals Potensi Bakteri Endofit Ubi Jalar (Ipomoea batatas L.) dalam Menghasilkan Hormon Indole Acetic Acid (IAA) dengan Penambahan L-triptofan

2020 ◽  
Vol 10 (1) ◽  
pp. 21
Author(s):  
Agustina Monalisa Tangapo
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Sweet Potato ◽  
Growth Regulators ◽  
Endophytic Bacteria ◽  
Ipomoea Batatas ◽  
Indole Acetic Acid ◽  
Iaa Production ◽  
Promote Plant Growth ◽  
Indole 3 Acetic Acid

Potensi Bakteri Endofit Ubi Jalar (Ipomoea batatas L.) dalam Menghasilkan Hormon Indole Acetic Acid (IAA) dengan Penambahan L-triptofan(Potential of endophytic bacteria of sweet potato (Ipomoea batatas L.) in producing Indole Acetic Acid (IAA) with the addition of L-tryptophan) Agustina Monalisa TangapoProgram Studi Biologi FMIPA Universitas Sam RatulangiJl. Kampus Unsrat, Manado 95115*Email korespondensi:[email protected] (Article History: Received 5-01-2019; Revised 15-01-2020; Accepted 05-02-2020) ABSTRAKAsosiasi bakteri-tanaman, dapat mempengaruhi produktivitas tanaman secara langsung dan tidak langsung. Secara langsung, salah satunya yaitu bakteri dapat memproduksi dan menyekresikan zat pengatur tumbuh indole-3-acetic acid (IAA, auksin). Penelitian ini bertujuan untuk menguji kemampuan bakteri endofit ubi jalar dalam menghasilkan IAA. Metode analisis IAA dilakukan dengan metode kolorimetri. Analisis produksi IAA dilakukan dengan penambahan dan tanpa penambahan L-triptofan. Hasil penelitian menunjukkan bahwa tanpa penambahan L-triptofan, diperoleh sejumlah 19 jenis yang menghasilkan IAA dengan kisaran konsentrasi 0,29-7,21 mg/L. Dengan penambahan L-triptofan, jumlah jenis positif dan konsentrasi IAA yang dihasilkan meningkat signifikan. Jumlah jenis positif 20 jenis (91%) dan konsentrasi IAA yang dihasilkan mencapai kisaran 0,96-115,63 mg/L.Kata kunci: bakteri endofit; IAA; ubi jalar; L-triptofan ABSTRACTPlant-bacteria associations, can promote plant growth by both direct and indirect mechanisms. One of direct mechanisms is that bacteria can produce and secrete indole-3-acetic acid (IAA, auxin) growth regulators. This study aims to examine the ability of sweet potato endophytic bacteria to produce IAA. The detection of IAA production was conducted by colorimetric technique. IAA production analysis was carried out with addition and without addition of L-tryptophan. Without the addition of L-tryptophan, a total of 19 species produced IAA with a concentration range of 0.29-7.21 mg/L. With the addition of L-tryptophan, the number of positive species and the concentration of IAA produced increased significantly. The number of positive species was 20 species (91%) and the concentration of IAA produced reached a range of 0.96-115.63 mg/L.Keywords: endophytes bacterial; IAA; sweet potato; L-tryptophan

Promotion of plant growth and inhibition of enzymic degradation of indole-3-acetic acid by metabolites of carbofuran, a carbamate insecticide

1977 ◽  
Vol 55 (5) ◽  
pp. 574-579 ◽  
Author(s):  
T. T. Lee
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Inhibitory Action ◽  
Critical Level ◽  
Growth Promotion ◽  
Stem Segment ◽  
Stem Tissue ◽  
Carbamate Insecticide ◽  
Promote Plant Growth ◽  
Indole 3 Acetic Acid

The carbamate insecticide carbofuran (2,2-dimethyl-2,3-dihydrobenzofuranyl-7-N-methyl carbamate) and three of its metabolites (7-hydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (III), 3,7-dihydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (IV), and 3-keto-7-hydroxy-2,2-dimethyl-2,3-dihydrobenzofuran (V)) stimulated growth in the pea stem segment assay in the presence, but not absence, of a low concentration of indole-3-acetic acid (IAA). The metabolites were more active than carbofuran itself. The synergistic effect on growth was specific with IAA since it was not observed in the presence of other auxins.Metabolites III, IV, and V and, to a lesser degree, carbofuran were found to be inhibitory to IAA degradation catalyzed by pea stem tissue or purified horseradish peroxidase (EC 1.11.1.7). Comparison of the relative activities of the compounds in the inhibition of IAA degradation and in the promotion of plant growth suggests a causal relationship. The implication is that carbofuran may promote plant growth through the inhibitory action of its metabolites on enzymic breakdown of IAA, thus preserving a critical level of IAA required for growth promotion.

Monoclonal antibodies to plant growth regulators. II. Indole-3-acetic acid

Planta ◽  
1985 ◽  
Vol 166 (3) ◽  
pp. 389-393 ◽  
Author(s):  
R. Mertens ◽  
J. Eberle ◽  
A. Arnscheidt ◽  
A. Ledebur ◽  
E. W. Weiler
Keyword(s):  
Acetic Acid ◽  
Monoclonal Antibodies ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Indole 3 Acetic Acid

scholarly journals Effect of Gibberellic Acid and Indole 3-Acetic Acid on Seed Germination Performance of Horse gram (Macrotyloma uniflorum) Lam (Verdc)

2016 ◽  
Vol 1 (2) ◽  
pp. 36 ◽  
Author(s):  
J. Lalitha ◽  
Haseena Rafath ◽  
M. Subash
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Germination Percentage ◽  
Potential Growth ◽  
Macrotyloma Uniflorum ◽  
Indole 3 Acetic Acid

The experiment was undertaken with an objective to investigate the effect of various concentrations of plant growth regulators, i.e., Gibberellic acid (GA3) and Indole 3-acetic acid (IAA) on seed germination of Macrotyloma uniflorum. Seeds were soaked for 12 hours in different concentrations substances (1.0, 2.0, 3.0 and 4.0 mg/L) of IAA, GA3 and control set was soaked only in distilled water.  Three replicates of each treatment with fifty seeds per replicate were arranged for precise physiological analysis. Significant variation was found in all aspects after analysis of variance (ANOVA) of each mean value. After two weeks of seed soaking, it was noted that germination percentages were significantly accelerated by lower concentrations (1 and 2 mg) of used hormones. Amongst the two potential growth regulators, 2 mg/L was found most effective because it showed highest germination percentage for IAA (93%) and GA3 (88%). A great deal of information relating to seed germination practices shows that these plant growth regulators were efficient in overcoming dormancy leading to rapid seed germination. IAA was selected as best hormone in this study, which showed highest seed germination (93%). 

scholarly journals Isolation and identification of indole acetic acid producing bacteria from the coasts of Ben Tre and Tra Vinh Provinces

2019 ◽  
Vol 41 (4) ◽  
Author(s):  
Nguyen Ngoc Lan ◽  
Vu Van Dzung ◽  
Nguyen Thi Kim Lien ◽  
Nguyen Kim Thoa ◽  
Do Huu Nghi ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Indole Acetic Acid ◽  
River Delta ◽  
Mekong River ◽  
Plant Growth Promoting Bacteria ◽  
Coastal Regions ◽  
Isolation And Identification ◽  
Mekong River Delta ◽  
Iaa Production

Beneficial plant-growth-promoting bacteria (PGPB) have been reasonably applied to rescue crucial issue for agriculture by salinity soil. Observed most of PGPB was found in endophyte, rhizosphere and soil. Indole acetic acid (IAA)-producing bacteria could naturally stimulate and facilitate plant growth. The knowledge of IAA production and content of bacteria resident in the marine environment has been typically insufficient and limited to date. In recent years, unwarrantable intrusions of sea water have been enlarged in the Mekong River Delta of Vietnam, threatening productive rice fields, local fruits, and cash crops. Therefore, finding PGPB in the coastal regions in the Mekong River Delta as a creative resource for sustainable agriculture is necessary and is a prompt challenge. In this study, IAA-producing bacteria from coastal regions of Ben Tre and Tra Vinh Provinces were isolated and adequately identified. Out of 202 bacterial isolates, 10 isolates showed the possible ability to produce IAA from L-tryptophan. These 10 isolates were objectively evaluated the capacity to produce IAA under 5% (w/v) NaCl in King B and marine broths. The results revealed that IAA production decreased in 5% NaCl, even though bacterial growth increased. These 10 IAA-producing bacteria were classified at the species level, Marinobacter hydrocarbonoclasticus, M. pelagius, M. daepoensis, and Mameliella phaeodactyli by 16S rRNA gene analysis. The most IAA producer in King’s B broth, the isolate C7, was investigated in more detail. The isolate C7 produced the maximum IAA amount (192.2 ± 1.14 µg/ml) under the presence of 20 g/l yeast extract, 2 g/l of L-tryptophan and 1% NaCl. The isolate C7 was able to grow at 1–17% (w/v) NaCl (optimum, 4%), but not in the absence of NaCl, indicating it is a moderate halophilic bacteria. This study highlighted the considerable ability to produce IAA of marine bacteria, which could be thoughtfully considered to use naturally as biofertilizers to promote plant growth in saline intrusion lands. 

scholarly journals Rhizospheric Fungi to Plant Growth Promotion: A Review

2021 ◽  
Vol 9 (1) ◽  
pp. 411
Author(s):  
João Manoel da Silva ◽  
Yamina Coentro Montaldo ◽  
Arthur Costa Pereira Santiago de Almeida ◽  
Viviane Araújo Dalbon ◽  
Juan Pablo Molina Acevedo ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Growth Regulators ◽  
Indole Acetic Acid ◽  
Growth Promotion ◽  
Direct Influence ◽  
Biological Interactions ◽  
Ecological Role ◽  
And Control ◽  
Stimulation Of

The rhizosphere is the region that has direct influence from the roots. This is the place where most of the interactions between microorganisms and plants occur. Studies involving the ecology of microbial communities from the rhizosphere became more frequent after the first reports of biological interactions of microorganisms with plants that influence physically and chemically modify the soil surrounding. According to these hypotheses, the rhizosphere mycobiota provides the development of plants through various mechanisms, direct and indirect. Thus, the objective of this review was to explain the aspects that provide characterizing these microorganisms as beneficial to plants in view of their applicability to agro-ecosystems. Therefore, it is stated that rhizospheric fungi have the solubilization of phosphorus (P), assimilating this nutrient for plants, promoting growth through the production or stimulation of the production of growth regulators such as 3-indole acetic acid, and control of phytopathogenic agents such as other filamentous fungi, and also phytonemamatodes. Therefore, it is possible to observe the importance of the constant observance of the action of these microorganisms in terms of their ecological role due to the agro-ecosystem.

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