scholarly journals Penggunaan ragi roti (Saccharomyces cerevisiae) sebagai imunostimulan untuk meningkatkan resistensi ikan mas (Cyprinus carpio L) terhadap infeksi bakteri Aeromonas hydrophila

2017 ◽  
Vol 4 (3) ◽  
Author(s):  
Henky Manoppo ◽  
Magdalena E.F. Kolopita

A research had been conducted to evaluate the use of baker’s yeast as immunostimulant in enhancing the resistance of carp (Cyprinus carpio L) to Aeromonas hydrophila infection.  Experimental fish were obtained from Freshwater Aquaculture Board in Talelu, Minahasa Regency, and transported to the Laboratory of Aquaculture Technology at the Faculty of Fisheries and Marine Science, Sam Ratulangi University. During two weeks of  acclimatization process, fingerlings were fed with commercial feed (pellet) at 5% of body weight per day, twice a day.  After acclimatization, fish was randomly distributed into 15 aquaria with a density of 25 fishes/aquarium. The doses of yeast cells as treatments included 5, 10, 15 and 20 g yeast/kg of pellet while control pellet not supplemented with yeast cell. Fish was fed with treatment pellets for four consecutive weeks at 5% of body weight per day,  twice daily at 09.00 am and 16.00 pm. Afterward, the density of fish were adjusted to 10 fish/aquarium. Fish were then challenged with A. hydrophila through intraperitoneal injection of  0.2 mL of A. hydrophila suspension containing 1x107 cfu/mL. Research result showed that supplementation of yeast cells into fish pellet significantly influenced the resistance of fish to A. hydrophila infection (p<0,01). The highest resistance were observed in fish fed pellet supplemented with 5 g yeast/kg of pellet. Thus, supplementation of  baker’s yeast cells into commercial fish feed was able to increase  resistance of carp to A. hydrophila infection.   Keywords: baker’s yeast, Cyprinus carpio, A. hydrophila, resistance, intraperitoneal injection

2017 ◽  
Vol 5 (2) ◽  
Author(s):  
Atiek P. Razak ◽  
Reni L. Kreckhoff ◽  
Juliaan Ch. Watung

The research aimed to evaluate the effect of baker’s yeast as immunostimulant on the growth of carp.  Fish was obtained from Board of Freshwater Aquaculture at Tatelu, North Sulawesi Province.  Before running the experiment, the fish was acclimatized for one week in five concrete tanks measuring 2x1x1 m3 each. During acclimatization, fish was fed commercial pellet at 5%/body weight/day, twice a day.  After acclimatizationm, the fish was distributed in 15 aquaria with a density of 25 individuals per aquarium.  The research used Complete Randomized Design with five treatments namely 0, 5, 10, 15, and 20 g yeast/kg of pellet, each with three replications.  Fish was fed with treatment pellets for three weeks consecutively at a dose of 5%/body weight/day, twice a day at 08.00 am and 16.00 pm. Growth of fish was measured at the end of feeding period.  The result showed that the addition of baker’s yeast cells into fish feed significantly influenced fish growth. The best growth was achieved in fish fed pellet supplemented with 5 g yeast/kg of pellet.Keywords : Cyprinus carpio, Saccharomyces cerevisiae, immunostimulant, growth


Author(s):  
Ida N Jamal ◽  
Reiny A Tumbol ◽  
Remy E.P Mangindaan

Motile Aeromonas Septicaemia disease (MAS) attacking tilapia has increased in recent years as a consequence of intensive aquaculture activities, which led to losses in aquaculture industry. The agent causing MAS disease is Aeromonas hydrophila. The disease can be controlled with the β-glucan. As immunostimulants, β-glucans can also increase resistance in farmed tilapia. Studies on the use of β-glucan extracted from baker's yeast Saccharomyces cerevisiae was intended to evaluate the non-specific immune system of tilapia that were challenged with Aeromonas hydrophila. The method used was an experimental method with a completely randomized design consisting of four treatments with three replicats. The dose of β-glucan used as treatments were 0 mg.kg-1 fish (Control), 5 mg.kg-1 fish (B), 10 mg.kg-1 fish (C) and 20 mg.kg-1 fish (D), each treatment as injected three times at intervals of 3 days, the injection volume of 0.5 ml/fish for nine days and resistance surveillance for seven days. The results showed that the difference in the amount of β-glucan and the frequency of the injected real influence on total leukocytes, phagocytic activity and resistance. Total leukocytes, phagocytic activity and resistance to treatment was best achieved by the administration of C a dose of  10 mg.kg-1 of the fish© Penyakit Motil Aeromonas Septicaemia (MAS) yang menyerang ikan nila mengalami peningkatan selama beberapa tahun terakhir sebagai konsekuensi dari kegiatan akuakultur intensif, yang menyebabkan kerugian dalam industri budidaya. Agen utama penyebab penyakit MAS adalah Aeromonas hydrophila. Untuk mengendalikan penyakit tersebut dapat dilakukan dengan pemberian β-glukan. Sebagai imunostimulan, β-glukan juga dapat  meningkatkan resistensi pada ikan nila yang dibudidayakan. Pengkajian mengenai pemanfaatan β-glukan yang diekstrak dari ragi roti Saccharomyces cerevisiae dimaksudkan untuk menguji sistem imun non spesifik ikan nila yang diuji tantang dengan bakteri Aeromonas hydrophila. Metode yang digunakan yaitu metode eksperimen dengan rancangan acak lengkap yang terdiri dari empat perlakuan dan tiga ulangan. Dosis β-glukan  yang digunakan sebagai perlakuan sebesar 0 mg.kg-1 ikan (Kontrol), 5 mg.kg-1 ikan (B), 10 mg.kg-1 ikan (C) dan 20 mg.kg-1 ikan (D), masing-masing perlakuan diinjeksi sebanyak 3 kali dengan interval waktu 3 hari selama 9 hari, volume injeksi 0,5 mL/ekor ikan dan pengamatan resistensi selama tujuh hari. Hasil penelitian menunjukkan perbedaan jumlah β-glukan dan frekuensi pemberian yang diinjeksikan memberikan pengaruh nyata terhadap total leukosit, aktivitas fagositosis dan resistensi. Total leukosit, aktivitas fagositosis dan resistensi terbaik dicapai pada perlakuan C dengan dosis 10 mg.kg-1 ikan©


1981 ◽  
Vol 45 (12) ◽  
pp. 2713-2721
Author(s):  
Youichi Tamai ◽  
Hiroshi Shinmoto ◽  
Masayoshi Takakuwa

2019 ◽  
Vol 143 ◽  
pp. 330-335
Author(s):  
Jonas Daci da Silva Serres ◽  
Pamela Taisline Bandeira ◽  
Paloma Cabral Zappani ◽  
Leandro Piovan ◽  
Marcos Lúcio Corazza

1988 ◽  
Vol 34 (8) ◽  
pp. 241-245 ◽  
Author(s):  
E. ŠTURDÍK ◽  
R. KOLLÁR ◽  
I. BERNÁT ◽  
M. MIKULÁŠOVÁ ◽  
J. FORSTHOFFER ◽  
...  

2011 ◽  
Vol 4 (2) ◽  
pp. 368-376 ◽  
Author(s):  
L. Mikoliunaite ◽  
A. Makaraviciute ◽  
A. Suchodolskis ◽  
A. Ramanaviciene ◽  
Y. Oztekin ◽  
...  

2016 ◽  
Vol 46 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Suzana Cardoso RIBEIRO ◽  
Antonielson Silva CASTELO ◽  
Bruna Marjara Picanço da SILVA ◽  
Andreza da Silva CUNHA ◽  
Aldo Aparecido PROIETTI JÚNIOR ◽  
...  

ABSTRACTIn fish farmings, diseases can be reduced by using immunostimulants. The aim of this study was to evaluate the immunostimulant potential of Mentha piperita in tambaqui fed with 0, 0.5, 1.0 and 1.5% of oil per kg of commercial fish feed. The fish were inoculated with Aeromonas hydrophila to challenge them. Hematological and biochemical parameters were determined after 30 days of feeding and seven days after the challenge. There was no mortality and M. piperita oil did not influence fish production parameters. However, blood hemoglobin concentration (Hb) increased in the fish fed with 0.5 and 1.5% of oil per kg of diet; albumin increased in those fed with 1.0%; cholesterol increased in all groups with oil; and triglycerides increased in those fed with 0.5%. After the bacterial challenge, the fish showed decreases in Hb when fed with diet enriched with 1.5% oil per kg of diet, in mean corpuscular volume with 1.0% and in mean corpuscular hemoglobin concentration with 0 and 1.5%. Protein levels increased in groups with 0 and 1.5% of oil and albumin when fed with 0 and 1.0%; cholesterol levels increased in the control group; and high levels of triglycerides were observed in the groups with 0, 0.5 and 1.5%. Thus, M. piperita essential oil promoted hematological alterations in tambaqui and can be recommended in diets containing up to 1.0% per kg, because of the minimal physiological modifications caused. However, additional studies are necessary to obtain more information regarding to the physiological effects of this immunostimulant.


Aquaculture ◽  
2012 ◽  
Vol 356-357 ◽  
pp. 169-175 ◽  
Author(s):  
G. Biswas ◽  
H. Korenaga ◽  
H. Takayama ◽  
T. Kono ◽  
H. Shimokawa ◽  
...  

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