scholarly journals PIGMEN KAROTENOID PADA KEPITING Ozius sp

2020 ◽  
Vol 8 (1) ◽  
pp. 47
Author(s):  
Sriyati Enjelina Sibarani ◽  
Darus Saadah Johanis Paransa ◽  
Kurniati Kemer ◽  
Desy M.H Mantiri ◽  
Natalie D.C Rumampuk ◽  
...  

Carotenoid pigments are natural dyes that have an influence on organism. For the human body, carotenoids function as antioxidants, anticancer, anti-bacteria and help maintain eye health. Carotenoid pigments are found in groups of bacteria, fungi, molds, algae and green plants and one of them in crustaceans is the crab Ozius sp. To determine the metabolic process of carotenoid pigments, each crab organ of Ozius sp. isolated using thin layer chromatography separation method to determine the type of pigment contained therein. The type of pigment identified in the crab Ozius sp. females with the TLC method namely: β-carotene, Cantanxhantine, and β-cryptosanthin.Keywords: Ozius sp, Thin Layer Chromatography, Carotenoids Pigments. Pigmen karotenoid adalah zat warna alami yang memiliki pengaruh terhadap organisme. Bagi tubuh manusia karotenoid berfungsi sebagai antioksidan, antikanker, anti bakteri dan membantu memelihara kesehatan mata. Pigmen karotenoid banyak ditemukan pada kelompok bakteri, jamur, kapang, ganggang dan tanaman hijau serta pada krustasea salah satunya yaitu pada kepiting Ozius sp. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Ozius sp. betina dengan metode KLT yaitu: β- karoten, Kantasantin, dan β- kriptosantin.Kata Kunci: Ozius sp, Kromatografi Lapis Tipis, Pigmen Karotenoid

2019 ◽  
Vol 7 (2) ◽  
pp. 104
Author(s):  
Dias Diasasthisa ◽  
Darus J Paransa ◽  
Desy MH Mantiri ◽  
Antonius Rumengan ◽  
Veibe Warouw ◽  
...  

Carotenoids are pigments with a range of red, orange and yellow colors. In carotenoid and chlorophyll plants are located in chloroplasts which undergo photosynthesis as well as photosynthetic bacteria and fungi. Carotenoid compounds have antioxidant activity, anticancer, as precursors of vitamin A and can enhance immunity. Crustaceans like crabs Grapsus sp. have carotenoid pigments, visible red, orange, and yellow are scattered in the carapace. Pigments found in crustaceans as well as crabs of Grapsus sp. generally sourced from food eaten by the crab. To determine the process of carotenoid pigment metabolism, each crab organ of Grapsus sp. isolated using thin layer chromatography separation method and column chromatography to determine the type of pigment contained therein. Pigment types identified in the crabs of Grapsus sp. males using the TLC method: β-carotene, Astasen type, Cantasantin, Astasantin, and Adonirubin. Pigment types identified in the crabs of Grapsus sp. males using the CC method: β-carotene, Astasen, β-cryptosanthine, Zeaxantine, and Cryptosanthine. Keywords : Grapsus sp., Thin Layer Chromatography, Column Chromatography, Carotenoids Pigments.             Karotenoid merupakan pigmen dengan kisaran warna merah, orange dan kuning. Pada tumbuhan karotenoid dan klorofil terletak pada kloroplas yang mengalami proses fotosintesis seperti juga pada bakteri fotosintetik dan fungi. Senyawa karotenoid memiliki aktivitas antioksidan, antikanker, sebagai prekursor vitamin A dan dapat meningkatkan imunitas. Krustasea seperti kepiting Grapsus sp. mempunyai pigmen karotenoid, terlihat warna merah, jingga, dan kuning yang tersebar pada karapas. Pigmen yang terdapat pada krustasea demikian juga pada kepiting Grapsus sp. umumnya bersumber dari makanan yang dimakan oleh kepiting tersebut. Untuk mengetahui proses metabolisme pigmen karotenoid maka masing-masing organ kepiting Grapsus sp. diisolasi dengan menggunakan metode pemisahan kromatografi lapis tipis dan kromatografi kolom untuk mengetahui jenis pigmen yang terkandung didalamnya. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KLT yaitu : β- karoten, Tipe Astasen, Kantasantin, Astasantin, dan Adonirubin. Jenis pigmen yang teridentifikasi pada kepiting Grapsus sp. jantan dengan metode KK  yaitu : β- karoten, Astasen, β-kriptosantin, Zeaxantin, dan Kriptosantin. Kata Kunci : Grapsus sp., Kromatografi Lapis Tipis, Kromatografi Kolom, Pigmen Karotenoid.  


1990 ◽  
Vol 38 (5) ◽  
pp. 517 ◽  
Author(s):  
B Czeczuga ◽  
G Kantvilas

Column and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 20 lichen species from Tasmanian forests: α-carotene, β-carotene, β -cryptoxanthin, lutein, zeaxanthin, 5-hydroxy-5,6-dihydrozeaxanthin, β -carotene epoxide, lutein epoxide, antheraxanthin,mutatoxanthin, violaxanthin, neoxanthin, α-doradexanthin, canthaxanthin and astaxanthin. 5-Hydroxy-5,6-dihydrozeaxanthin is reported for the first time in lichens. The total content of carotenoids ranged from 15.28�g-1 dry wt in Menegazzia confusa to 48.53�g-1 dry wt in Pseudocyphellaria glabra.


1977 ◽  
Vol 32 (5-6) ◽  
pp. 442-443
Author(s):  
P. Fähnrich ◽  
L. Chenaux

Carotenoids were extracted from gametophytes of Allomyces arbuscula at different periods during gametogenesis. After separation by thin-layer chromatography γ-carotene, lycopene and β-carotene were identified from their absorp­tion spectra in visible light. No other carotenoids were detected. After a lag period of 30 min following induction of gametogenesis γ-carotene was the only carotenoid present in extracts, followed by traces of β-carotene at 60 min and by lycopene at 90 min. Accumulation of carotenoids continued during the later stages of gametangia development. Analysis of pigments that had been extracted from asexual mitospores showed that these spores contained both γ-carotene and lycopene.


Eisei kagaku ◽  
1991 ◽  
Vol 37 (6) ◽  
pp. 542-551 ◽  
Author(s):  
FUMIO MIYAMOTO ◽  
MASANOBU SAEKI ◽  
MASAYA KAMIJO ◽  
HIROSHI KANDA ◽  
TADAYOSHI NAKAOKA ◽  
...  

1968 ◽  
Vol 46 (12) ◽  
pp. 1539-1541 ◽  
Author(s):  
A. U. Alam ◽  
J. R. Couch ◽  
C. R. Creger

Pigments of Tagetes erecta petals have been separated by the use of multiple fractional extractions and thin-layer chromatography and analyzed by spectral methods. The petals showed a predominance of xanthophylls (98.7%) and only a small amount of carotenes. On the basis of absorption studies, lutein (64.1%), antheraxanthin (31.1 %), α-cryptoxanthin (3.1%), (β-carotene (0.6%), phytofluene (0.4%), and α-carotene (0.15%) have been characterized.


2014 ◽  
Vol 54 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Bazyli Czeczuga

By means of column and thin-layer chromatography, the author investigated tit presence of various carotenoids in stems of 3 species of the Sphagnum genus. Apocarotenals (β-apo-2', β-apo-10' -carotenal and apo-12' -violaxanthal) and the following carotenoids were found: α-, β-, ;-carotene, β-cryptoxantin, lutein, lutein epoxide, zeaxanthin, adonixanthin, antheroxanthin, rhodoxanthin, rubixanthin, neoxanthin, vio-laxanthin and mutatoxanthin. The total content of carotenoids ranged from 11.954 to 41.579 mg•g<sup>-1</sup> dry weight.


2014 ◽  
Vol 16 (1) ◽  
pp. 115-120 ◽  
Author(s):  
Bazyli Czeczuga

The autor used column and thin-layer chromatography to determine the occurrence and quantitative relationships of particular carotenoids in fruit-bodies of the <i>Calocera viscosa</i> and <i>Dacrymyces deliquescens</i>. The predominant carotenoid was β-carotene and its derivatives.


2014 ◽  
Vol 63 (1) ◽  
pp. 21-24 ◽  
Author(s):  
Bazyli Czeczuga

Column-, and thin-layer chromatography revealed the presence of the following carotenoids in the thalli of 29 lichen species from Białowieża Forest: α-carotene, β-carotene, α-cryptoxanthin, β-cryptoxanthin, lutein, 3'-epilutein, zeaxanthin, β-carotene monoepoxide, lutein epoxide, antheraxanthin, 3'-hydroxyechinenone, α-doradexanthin, canthaxanthin, astaxanthin, neoxani thin, violaxanthin and mutatoxanthin. The total content of carotenoids ranged from 16.83 (<i>Cladonia rangiferina</i>) to 92.98 µg g dry wt (<i>Xanthoria parietina</i>). There were differences in carotenoid composition, concentration of each carotenoid, and in the total content in the thalli of four species collected from niches with different insolation.


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