Perbedaan Penyembuhan Hecting Wound Tikus Putih Jantan Sprague Dawley dengan Wharton’s Jelly Dan D Gel

Pendahuluan; Luka post hecting adalah luka yang terjadi akibat tindakan medis, secara fisiologis tubuh akan mengalami proses penyembuhan luka. D gel merupakan gel yang mengandung siloxane cyclic dan vitamin C yang dapat digunakan untuk penyembuhan luka post hecting, tetapi salah satu pengobatan luka lain yang saat ini dapat digunakan adalah ekstrak sel punca mesenkimal tali pusat manusia (WJMSCs). Tujuan; mengetahui waktu perbedaan penyembuhan luka post hecting antara ekstrak WJMSCs dengan D gel. Metode; menggunakan penelitian eksperimental laboratorik yang menggunakan 21 ekor tikus putih jantan (Rattus novergicus) galur Sprague dawley yang dikelompokkan menjadi tiga kelompok perlakuan berbeda. Perlakuan dibagi atas kelompok K: kontrol negatif (povidone iodine), P1: ekstrak WJMSCs, dan P2: D gel. Pengamatan terhadap luka post hecting dilakukan selama 14 hari menggunakan kriteria Nagaoka dan data dianalisis menggunakan uji statistik deskriptif kategorik serta Kruskal-Wallis. Hasil; menunjukkan adanya perbedaan waktu penyembuhan luka post hecting antara ekstrak WJMSC dengan D gel secara bermakna dengan p value= 0,03, dengan waktu penyembuhan luka kelompok K: 12,7 hari, kelompok P1: 7 hari, dan kelompok P2: 11 hari. Kesimpulan; bahwa terdapat perbedaan bermakna penyembuhan luka post hecting antara ekstrak WJMSC dengan D gel

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Intrathecal Injection in a Rat Model: A Potential Route to Deliver Human Wharton’s Jelly-Derived Mesenchymal Stem Cells into the Brain

International Journal of Molecular Sciences ◽

10.3390/ijms21041272 ◽

2020 ◽

Vol 21 (4) ◽

pp. 1272 ◽

Cited By ~ 1

Author(s):

Hyeongseop Kim ◽

Duk L. Na ◽

Na Kyung Lee ◽

A Ran Kim ◽

Seunghoon Lee ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Rat Model ◽

Intrathecal Injection ◽

Wharton’S Jelly ◽

Sprague Dawley ◽

Wharton's Jelly ◽

Sprague Dawley Rats ◽

The Brain ◽

Underlying Pathology

Mesenchymal stem cells (MSCs) are considered as promising therapeutic agents for neurodegenerative disorders because they can reduce underlying pathology and also repair damaged tissues. Regarding the delivery of MSCs into the brain, intravenous and intra-arterial routes may be less feasible than intraparenchymal and intracerebroventricular routes due to the blood–brain barrier. Compared to the intraparenchymal or intracerebroventricular routes, however, the intrathecal route may have advantages: this route can deliver MSCs throughout the entire neuraxis and it is less invasive since brain surgery is not required. The objective of this study was to investigate the distribution of human Wharton’s jelly-derived MSCs (WJ-MSCs) injected via the intrathecal route in a rat model. WJ-MSCs (1 × 106) were intrathecally injected via the L2-3 intervertebral space in 6-week-old Sprague Dawley rats. These rats were then sacrificed at varying time points: 0, 6, and 12 h following injection. At 12 h, a significant number of MSCs were detected in the brain but not in other organs. Furthermore, with a 10-fold higher dose of WJ-MSCs, there was a substantial increase in the number of cells migrating to the brain. These results suggest that the intrathecal route can be a promising route for the performance of stem cell therapy for CNS diseases.

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Vitamin C reverses bone loss in an osteopenic rat model of osteoporosis

International Journal for Vitamin and Nutrition Research ◽

10.1024/0300-9831/a000486 ◽

2018 ◽

Vol 88 (1-2) ◽

pp. 58-64 ◽

Cited By ~ 4

Author(s):

Farzad Deyhim ◽

Kimberly Strong ◽

Niaz Deyhim ◽

Sarvenaz Vandyousefi ◽

Alexis Stamatikos ◽

...

Keyword(s):

Bone Loss ◽

Vitamin C ◽

Antioxidant Capacity ◽

Bone Quality ◽

Antioxidant Properties ◽

Ovariectomized Rats ◽

P Value ◽

Tartrate Resistant Acid Phosphatase ◽

Sprague Dawley ◽

Alp Activity

Abstract. Fruits and vegetables are rich in vitamin C with antioxidant properties which are known to influence bone quality. This study evaluated whether vitamin C (1000 mg/L) added to drinking water reverses the bone loss in ovariectomized rats. Ninety-day-old female Sprague-Dawley rats were randomly assigned to either sham (n = 14) or ovariecotmized groups (n = 28). Sixty days after ovariectomy, the treatments were sham, ovariectomy (OVX), OVX + vitamin C (22 mg oral intake daily) for 60 days. Urine was collected for deoxypyridinoline (DPD) evaluation, rats were sacrificed, and antioxidant capacity, osteopontin, alkaline phosphatase (ALP), and bone specific tartrate resistant acid phosphatase (TRAP) were evaluated in the plasma. Right femur and 5th lumbar were evaluated for bone density, strength, ash, Ca, and Mg concentrations. Antioxidant capacity, ALP activity, osteopontin decreased (p-value < 0.05), while TRAP and urinary DPD increased (p-value < 0.05) with ovariectomy. In contrast, vitamin C increased (p-value < 0.05) antioxidant capacity, ALP activity, osteopontin concentration and reduced (p-value < 0.05) TRAP and urinary DPD excretion, respectively. Ovariectomy reduced (p-value < 0.05) bone quality, bone ash, Ca and Mg concentrations. Vitamin C increased (p-value < 0.05) femoral density without affecting (p-value > 0.1) femoral strength, ash, or Ca, and Mg concentrations, while it increased (p-value < 0.05) the 5th lumbar density, ash, and Ca and Mg concentrations. In conclusion, vitamin C increased bone quality and antioxidant capacity in ovariectomized rats.

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An Injectable Hyaluronan–Methylcellulose (HAMC) Hydrogel Combined with Wharton’s Jelly-Derived Mesenchymal Stromal Cells (WJ-MSCs) Promotes Degenerative Disc Repair

International Journal of Molecular Sciences ◽

10.3390/ijms21197391 ◽

2020 ◽

Vol 21 (19) ◽

pp. 7391 ◽

Cited By ~ 1

Author(s):

Un Yong Choi ◽

Hari Prasad Joshi ◽

Samantha Payne ◽

Kyoung Tae Kim ◽

Jae Won Kyung ◽

...

Keyword(s):

Wharton’S Jelly ◽

Sprague Dawley ◽

Nucleus Pulposus Cells ◽

Degenerative Disc ◽

Wharton's Jelly ◽

Sprague Dawley Rats ◽

Disc Repair ◽

Substantial Progress ◽

Ivd Degeneration

Intervertebral disc (IVD) degeneration is one of the predominant causes of chronic low back pain (LBP), which is a leading cause of disability worldwide. Despite substantial progress in cell therapy for the treatment of IVD degeneration, significant challenges remain for clinical application. Here, we investigated the effectiveness of hyaluronan–methylcellulose (HAMC) hydrogels loaded with Wharton’s Jelly-derived mesenchymal stromal cell (WJ-MSCs) in vitro and in a rat coccygeal IVD degeneration model. Following induction of injury-induced IVD degeneration, female Sprague-Dawley rats were randomized into four groups to undergo a single intradiscal injection of the following: (1) phosphate buffered saline (PBS) vehicle, (2) HAMC, (3) WJ-MSCs (2 × 104 cells), and (4) WJ-MSCs-loaded HAMC (WJ-MSCs/HAMC) (n = 10/each group). Coccygeal discs were removed following sacrifice 6 weeks after implantation for radiologic and histologic analysis. We confirmed previous findings that encapsulation in HAMC increases the viability of WJ-MSCs for disc repair. The HAMC gel maintained significant cell viability in vitro. In addition, combined implantation of WJ-MSCs and HAMC significantly promoted degenerative disc repair compared to WJ-MSCs alone, presumably by improving nucleus pulposus cells viability and decreasing extracellular matrix degradation. Our results suggest that WJ-MSCs-loaded HAMC promotes IVD repair more effectively than cell injection alone and supports the potential clinical use of HAMC for cell delivery to arrest IVD degeneration or to promote IVD regeneration.

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