scholarly journals Estudo histológico da penetração do estilete da Pérola da Terra Eurhizococcus brasiliensis (Wille, 1922) (Hemiptera:Margarodidae) nas Raízes de Videira

ScientiaTec ◽  
2020 ◽  
Vol 7 (03) ◽  
Author(s):  
Marcelina Mezzomo Debiasi ◽  
Márcia Bündchen ◽  
Katiane Paula Bagatini ◽  
Edegar Luiz Peruzzo ◽  
Fernanda Maurer D´Agostini
Keyword(s):  

Eurhizococcus brasiliensis (Wille, 1922), a pérola da terra, é considerada a principal praga da videira no sul do Brasil e existe pouca informação sobre as interações inseto-planta. O objetivo deste estudo foi verificar o mecanismo de penetração de E. brasiliensis nas raízes de Vitis vinifera através de cortes histológicos. Amostras de 250 raízes de V. vinifera infestadas por E. brasiliensis foram coletadas e fixadas em FAA70 e armazenadas em etanol 70%; Após a fixação das raízes infestadas, os segmentos foram desidratados, emblocados em parafina, seccionados e corados para a preparação de lâminas histológicas permanentes. As lâminas foram analisadas em microscópio de luz e os dados obtidos do estudo anatômico revelaram que o estilete de E. brasiliensis quando introduzido nos tecidos radiculares rompe as células e aproxima-se do xilema. A penetração do estilete forma um canal com as margens mais claras e irregulares atingindo até 650 µm no interior dos tecidos da raiz.

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
P Bolfa ◽  
F Sarac ◽  
A Filip ◽  
A Gal ◽  
M Taulescu ◽  
...  

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
H Amira-Guebailia ◽  
T Richard ◽  
S Rouaiguia ◽  
P Waffo Tueguo ◽  
JC Delaunay ◽  
...  

Author(s):  
R. Preethi ◽  
P. Padma

The study focused on the green synthesis of silver nanobioconjugates (AgNPs) from phenolic-rich fruit source, Vitis vinifera seed extract and its major component phenolic, resveratrol respectively. Sunlight exposure for 20 minutes was the method of choice for the synthesis of AgNPs of the extract as well as the phenolic, resveratrol. The synthesized nanobioconjugates were characterized using UV-Visible spectroscopy, Transmission electron microscopy (TEM), Energy dispersive X-ray analysis (EDAX), X-ray diffraction (XRD), Polydispersity index, Zeta potential and Fourier transform infrared spectroscopy (FTIR). The reduction of silver ions was confirmed by UV-visible spectroscopy with peaks at 440nm for both nanobioconjugates synthesized from seed extract and compound. The nanobioconjugates showed the spherical in shape with 14-35nm in size and crystalline in nature. The conjugates are well dispersed with 0.301 and 0.287 polydispersity index and the zeta potential range at -13.6 and -14.3mV for stability. The FTRI data proved that the components in grape seeds act as good reductants and stabilizers for the silver nanobioconjugate synthesis. All the synthesized nanobioconjugates exhibited steady and sustained release of the medicinal components conjugated, proving their druggability, and were biocompatible with human cells, demonstrating their safety. The findings of the study validate the anticancer properties of silver nanobioconjugates of Vitis vinifera and its active component resveratrol.


2017 ◽  
Vol 40 (1) ◽  
pp. 95-102 ◽  
Author(s):  
B. Wang ◽  
T. Zhou1 ◽  
K. Li ◽  
X.W. Guo ◽  
Y.S. Guo ◽  
...  

2020 ◽  
Author(s):  
KJ Nunan ◽  
Ian Sims ◽  
A Bacic ◽  
SP Robinson ◽  
GB Fincher

Cell walls have been isolated from the mesocarp of mature grape (Vitis vinifera L.) berries. Tissue homogenates were suspended in 80% (v/v) ethanol to minimise the loss of water-soluble wall components and wet-sieved on nylon mesh to remove cytoplasmic material. The cell wall fragments retained on the sieve were subsequently treated with buffered phenol at pH 7.0, to inactivate any wall-bound enzymes and to dislodge small amounts of cytoplasmic proteins that adhered to the walls. Finally, the wall preparation was washed with chloroform/methanol (1:1, v/v) to remove lipids and dried by solvent exchange. Scanning electron microscopy showed that the wall preparation was essentially free of vascular tissue and adventitious protein of cytoplasmic origin. Compositional analysis showed that the walls consisted of approximately 90% by weight of polysaccharide and less than 10% protein. The protein component of the walls was shown to be rich in arginine and hydroxyproline residues. Cellulose and polygalacturonans were the major constituents, and each accounted for 30-40% by weight of the polysaccharide component of the walls. Substantial varietal differences were observed in the relative abundance of these two polysaccharides. Xyloglucans constituted approximately 10% of the polysaccharide fraction and the remainder was made up of smaller amounts of mannans, heteroxylans, arabinans and galactans.


Agrometeoros ◽  
2018 ◽  
Vol 25 (2) ◽  
Author(s):  
Cristina Pandolfo ◽  
Marilene De Lima ◽  
Angelo Mendes Massignam ◽  
Aparecido Lima da Silva ◽  
Luiz Albano Hammes

Os sistemas atmosféricos exercem um papel significativo no clima de uma região e poucos trabalhos determinaram os impactos dos sistemas atmosféricos no de­senvolvimento e na produção de videira. Os objetivos deste trabalho foram deter­minar a frequência de ocorrência dos sistemas atmosféricos durante o período da maturação à colheita da videira (Vitis vinifera L.) var. Cabernet Sauvignon para as duas regiões produtoras de Santa Catarina em diferentes safras e determinar a as­sociação entre a ocorrência dos sistemas atmosféricos e as regiões produtoras e as safras. Os sistemas atmosféricos foram identificados durante as safras de 2005/2006 à 2008/2009. Os municípios de Água Doce e Campos Novos foram escolhidos para re­presentar a região produtora 1 e os municípios de Bom Retiro e São Joaquim para re­presentar a região produtora 2. As frequências de ocorrências dos sistemas atmosfé­ricos durante o período da maturação à colheita de videira são muito semelhantes entre as regiões, somente houve diferença na frequência de ocorrência do sistema jato subtropical entre regiões. Houve uma diferença significativa da ocorrência da frequência dos sistemas atmosféricos entre safras.


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