Antioxidant Activity and Protective Effect of Extracts from Vitis vinifera Root on t-BHP Induced Oxidative Stress in Chang Cells

Herbal tea with antioxidant ingredients has gained increasing attention in the field of functional foods due to their amelioration potential in aging-related diseases. Wanglaoji herbal tea (WHT) is a kind of traditional beverage made from herbal materials. This study was performed to investigate its antioxidant activity and identify its protective effect on a H2O2-induced cell damage model. In this study, we identified six kinds of phenolic acids with antioxidant activity in WHT, among which rosmarinic acid had the highest content and the highest contribution ratio to the antioxidant activity of WHT. Moreover, compared with the H2O2-induced damage group, the WHT treatment group can significantly increase the viability of cells and decrease the ratio of senescence-associated β-galactosidase-positive cells, intracellular malondialdehyde levels, and the percentage of G1 phase. Furthermore, enrichment analysis of differentially expressed genes revealed that heme oxygenase1 (HMOX1) was a key gene for protective effect of WHT on oxidative stress-induced cell damage. Thus, WHT exerted protective effects not only by scavenging reactive oxygen species but also by inducing the expression of cytoprotective genes by activating the HMOX1 pathway, which showed that WHT had a potential of promoting health by reducing oxidative stress-induced cell damage.

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Protective effect of Öküzgözü (Vitis vinifera L. cv.) grape juice against carbon tetrachloride induced oxidative stress in rats

Food & Function ◽

10.1039/c2fo30024a ◽

2012 ◽

Vol 3 (6) ◽

pp. 668 ◽

Cited By ~ 13

Author(s):

Mihdiye Pirinççioğlu ◽

Göksel Kızıl ◽

Murat Kızıl ◽

Gültekin Özdemir ◽

Zeki Kanay ◽

...

Keyword(s):

Oxidative Stress ◽

Carbon Tetrachloride ◽

Protective Effect ◽

Vitis Vinifera ◽

Grape Juice ◽

Vitis Vinifera L

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Methoxy VO-salen complex: In vitro antioxidant activity, cytotoxicity evaluation and protective effect on CCl4-induced oxidative stress in rats

Food and Chemical Toxicology ◽

10.1016/j.fct.2008.12.029 ◽

2009 ◽

Vol 47 (4) ◽

pp. 716-721 ◽

Cited By ~ 22

Author(s):

Mohsen Mohammadi ◽

Razieh Yazdanparast

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Protective Effect ◽

Salen Complex ◽

In Vitro Antioxidant Activity

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Preparation, characterization, antioxidant activity and protective effect against cellular oxidative stress of phosphorylated polysaccharide from Cyclocarya paliurus

Food and Chemical Toxicology ◽

10.1016/j.fct.2020.111754 ◽

2020 ◽

Vol 145 ◽

pp. 111754

Author(s):

Liuming Xie ◽

Mingyue Shen ◽

Pingwei Wen ◽

Yuzhen Hong ◽

Xuan Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Protective Effect ◽

Cyclocarya Paliurus

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Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models

Molecules ◽

10.3390/molecules25215149 ◽

2020 ◽

Vol 25 (21) ◽

pp. 5149

Author(s):

Roberto O. Ybañez-Julca ◽

Daniel Asunción-Alvarez ◽

Ivan M. Quispe-Díaz ◽

Javier Palacios ◽

Jorge Bórquez ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Protective Effect ◽

High Performance ◽

Mangifera Indica ◽

Folk Medicine ◽

In Vitro Assays ◽

Antispasmodic Activity ◽

Intestinal Segments

Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p < 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10−5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p < 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p < 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg/kg) for 28 days significantly reduced (p < 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.

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