scholarly journals INFLUENCE OF CYTOKININS AND SUCROSE CONCENTRATIONS ON BULBLET CHARACTERS OF IN VITRO HIPPEASTRUM HYBRIDUM

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
Al-Amery & Salman
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Dry Weight ◽  
Culture Technique ◽  
College Of Agriculture ◽  
Tissue Culture Technique

The First Part of this study was conducted at the Plant Tissue Culture Lab at the College of Science, University of Nahrain from October 2014 to February,2015. and completed at the Plant Tissue Culture Lab at the College of Agriculture, University of Baghdad From February 2015 to September 2015. Examine the possibility of using the tissue culture technique in the propagation of Hippeastrum  hybridum.plantlets were resulted from leaves induced plant lets moved to new MS media supplemented with BA and Kin at 2.0, 1.0, 0.5, 0.0 mg.liter-1 individually or in combination and with or without NAA at 0.5, 0.3, 0.1, 0.0 mg.liter-1 to enhance shoot proliferation. Transferred shoot  from the best proliferation- enhance to stage bulbs formation, MS media supplemented with BA at 6.0, 3.0, 1.5, 0.0 0  mg.liter-1 in addition to sucrose at  90, 60, 300 g.liter-1 with the present of NAA at 0.1 mg.liter-1 to increase bulbs formation, weight, and diameter .Result showed that the best  shoot proliferation media was MS supplemented with 1.0 mg.liter-1 BA and 0.3 mg.liter-1 NAA which resulted in 8.30 shoots.plant-1. As for bulbs formation, the results exhibited that MS media supplemented with  6.0  mg.liter-1 BA and 90 g.liter-1 sucrose with the existence of 0.10 mg.liter-1 NAA gave the highest bulbs formation percentage, diameter, and both fresh and dry weight which were 3 bulbs.explant-1, 0.98 cm, and 1.04 and 0.25 g, respectively.

scholarly journals STUDY THE EFFECT OF CYTOKININS AND AUXINS IN THE COMPOSITION AND PRODUCTION OF IN VITRO PLANTLETS HIPPEASTRUM HYBRIDUM

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
Al-Amery & Salman
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Culture Technique ◽  
College Of Agriculture ◽  
Best Response ◽  
Response Profile ◽  
Half Strength

The First Part of this study was conducted in the Plant Tissue Culture Lab at the College of Science, University of Nahrain from October 2014 to February,2015. The experiment was then completed in the Plant Tissue Culture Lab at the College of Agriculture, University of Baghdad From February 2015 to September 2015. Examine the possibility of using the tissue culture technique in the propagation of Hippeastrum  hybridum. Explants (Bulbs, Leaves) had been sterilized using four different NaOCL concentrations: % 4.0, 2.0, 1.0, 0.0. After sterilization, explants were cultured on MS media supplemented with BA at four concentrations (2.0, 1.0, 0.5, 0.0 mg.liter-1) and NAA at four concentrations (0.5, 0.3, 0.1, 0. 0 mg.liter-1) to obtain plantlets. Afterwards, plantlets were moved to new MS media supplemented with BA (2.0, 1.0, 0.5, 0.0 mg.liter-1) and with or without four concentrations of NAA (0.5, 0.3, 0.1, 0.0 mg.liter-1) to enhance shoot proliferation. The resulted shoots from the best proliferation-enhance media were divided into two parts the first part was transferred to root-promoting media which also included two experiments where the first experiment was by transferring the shoots to MS media (half and full strength) supplemented with NAA at four concentrations (0.5, 0.3, 0.1, and 0.0  mg.liter-1). The second experiment included the best resulted shoots from the first experiment in addition to (0 and 2 mg.liter-1) actived charcoal to increase root percentage, root count, and root length.The resulted plants were acclimatized using peatmoss/soli mixture at the ratio of 1:1, 2:1, and 1:2 to obtain high surviving ratio. Results showed that the best explant(Bulbs, Leaves) was soaking in 3% NaOCl for 10 min. the results also showed that the leaves gave best response 90% when using MS media supplemented with 1.0 mg.liter-1  BA and 0.3 mg.liter-1 NAA when compared with the bulbs that showed low response profile. Furthermore, the best shoot proliferation media was MS supplemented with 1.0 mg.liter-1 BA and 0.3 mg.liter-1 NAA which resulted in 8.30 shoots.plant-1. The best rooting percentage was obtained when culturing the shoots in half strength MS media supplemented with 0.5 mg.liter-1 NAA with the existence of activated charcoal. The surviving percentage reached 95% when using the previously mentionedpeatmoss-soil/mixtures.  

scholarly journals An Overview of Oil Palm Cultivation via Tissue Culture Technique

2021 ◽  
Author(s):  
Siti Khadijah A. Karim
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Oil Palm ◽  
Plant Tissue ◽  
Culture Technique ◽  
Mitigation Measures ◽  
Culture Techniques ◽  
Tissue Culture Technique ◽  
Tissue Culture Techniques

During the last three decades, plant cell, tissue, and organ culture have developed rapidly and become a major biotechnology tool in agriculture, horticulture, forestry, and industry. Many problems in conventional breeding techniques were solved via tissue culture techniques. Plant tissue culture technique permits the growing plants in test tube or closed container in vitro under controlled environment. This technique is devoted to solve two problems: 1) To keep the plant cells free from microbes. 2) To grow the desired plants by providing suitable nutrient medium and other environmental conditions. In this chapter, a review around plant tissue culture techniques that have been reported on oil palm breeding programme will be discussed. It is including the laboratory techniques, advantages and disadvantages of the technique, the problems to produce good and prolific oil palm tissue culture clones and mitigation measures that have been reported to overcome the problems. As a conclusion, this chapter reviews tissue culture techniques that could be used to propagate oil palm clones.

scholarly journals In Vitro Micropropagation of Lilium candidum Bulb by Application of Multiple Hormone Concentrations Using Plant Tissue Culture Technique

2021 ◽  
Vol 8 (2) ◽  
pp. 244-253
Author(s):  
Akshay Milind Patil ◽  
Pooja Prakash Gunjal ◽  
Dr. Sonali Das
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Health Benefits ◽  
Vitamin B1 ◽  
Scar Tissue ◽  
Culture Technique ◽  
Therapeutic Uses ◽  
Micro Propagation

The multiplication efficacy by bulb is low and the plantlets are more susceptible to disease, therefore, there is a need to develop a protocol for its propagation. Lilium candidum is listed in the saitma prefecture Red Data Book as a critically endangered plant and rescuing information regarding its micro-propagation is rather limited. On this regard, the application of in vitro micropropagtion procedure might help to obtain large numbers of uniform plants of endangered species of Lilium. Dried lilies are a rich source of fiber and also rich in sodium and carbs. Lily bulbs have proteins and starch and also small quantities of iron, calcium, phosphorous, and vitamin B1, B2, C. The health benefits of the lily for the heart are well known on account of the active cardiac glycosides as well as the flavonoids which tend to stimulate the arteries and can cause them to dilute. Another one of the therapeutic uses of the lily flower is in the case of treating burns and preventing the formation of scar tissue. One of the main health benefits of the lily flower is that it helps regulating the heart rate there by allowing the heart to function more efficiently and regular. Having multiple medicinal properties we decided to cultivate Lilium candidum using plant tissue culture so farming can be increased using this cost efficient techniques. In this research, we have studied various Effect of different concentration of BAP and NAA on the initiation of Lilium candidum from bulb and IBA, IAA and NAA on the rooting of shoots of Lilium Candidum.

scholarly journals In vitro growth of Brassocattleya orchid hybrid in different concentrations of KNO3, NH4NO3 and benzylaminopurine

2011 ◽  
Vol 29 (3) ◽  
pp. 359-363 ◽  
Author(s):  
Jean C Cardoso ◽  
Elizabeth O Ono
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Ornamental Plants ◽  
Dry Weight ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Mass Propagation ◽  
Mineral Salts

One of the most important applications of plant tissue culture is mass propagation of ornamental plants. This experiment evaluated the effect of different concentrations of NH4NO3 and KNO3 and BAP on the in vitro growth of orchid hybrid Brassocattleya 'Pastoral'. Seedlings of this orchid hybrid were used as explants and cultivated in medium with mineral salts and vitamins from the MS medium (Murashige & Skoog, 1962), with the macronutrients P, Ca and Mg reduced by half, and with an addition of 25 g L-1 of sucrose, 0.1 g L-1 of myo-inositol and 1.5 g L-1 of activated charcoal. Agar-agar was added (6.5 g L-1) and the pH was adjusted to 5.8. As treatments, four concentrations of the NH4NO3 and KNO3 (2x; 1x; ½ and ¼ MS medium) and three concentrations of BAP (0.0; 0.5 and 1.0 mg L-1) were assayed. The multiplication, growth in height, fresh and dry weight and sugar level in dry weight of sprouts were evaluated. There occurred a higher growth in height with 0.25x NH4NO3 and KNO3 salts concentrations of MS medium and higher rate of multiplication with combination of NH4NO3 and KNO3 reduced by half of the MS medium concentration and 1.0 mg L-1 BAP.

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

scholarly journals PRIMING OF GENETIC VARIATIONS BY COLOCYNTH FRUIT EXTRACT AND CALLUS INITIATION IN EMBRYOS OF TWO CULTIVARS OF ALFALFA SEEDS

2017 ◽  
Vol 48 (3) ◽  
Author(s):  
Hamza & Ali
Keyword(s):  
Tissue Culture ◽  
Dry Weight ◽  
Culture Technique ◽  
Factorial Experiment ◽  
Graduate Studies ◽  
College Of Agriculture ◽  
Fruit Extract ◽  
Local Variety ◽  
Molecular Weights ◽  
Callus Initiation

An experiment  was conducted at Central lab of Graduate studies, College of Agriculture, University of Baghdad during 2015-2016. The aim was to study the effect of colocynth fruit extract on inducing callus from embryos of two alfalfa cultivars were PAC-78001 and local variety by tissue culture technique using factorial experiment within CRD. Seeds was soaked with colocynth fruit extract at 0, 75, 150, 225, and 300 ml Li-1 for 24 hours, then, sterilized by NaOCl at 4.5% for 15 minutes. The results showed significant differences between two cultivars responding to colocynth fruit extract levels. Concentration at 150 ml Li-1 gave the highest both fresh and dry weight of callus (205.90 and 20.60) respectively. Also, PCR results depending on RAPD and electrophoresis for DNA samples which isolated from callus of two cultivars and subjected to various concentrations of colocynth fruit extract 0, 75, 150, 225 and 300 ml Li-1 showed differences in amplified observed bands, molecular weights and brightness intensity by using primers primer -Y07, -Y06, OE-12, C-08, D-10, -Y10 and A-16, and There was no difference in bands number at all concentrations of colocynth fruit extract for local variety by primer A-16.       

scholarly journals In vitro plant tissue culture: means for production of biological active compounds

Planta ◽  
2018 ◽  
Vol 248 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Claudia A. Espinosa-Leal ◽  
César A. Puente-Garza ◽  
Silverio García-Lara
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Compounds ◽  
In Vitro Plant

scholarly journals Endogenous Bacterial Contamination of Plant Tissue Culture Materials: Identification and Control Strategy

2018 ◽  
Vol 28 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Mohammad Ali ◽  
Shefali Boonerjee ◽  
Mohammad Nurul Islam ◽  
Mihir Lal Saha ◽  
M Imdadul Hoque ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacterial Contamination ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Bacterial Isolates ◽  
Culture And Sensitivity ◽  
And Control

The endogenous bacterial contamination of plant tissue culture materials and their possible control was studied. Nine bacterial isolates were isolated from the contaminated tissue culture materials viz. potato and tea. On the basis of morphology and biochemical characters of nine isolates, seven were identified as Gram positive belonging to Bacillus alcalophilus, B. circulans, B. infantis, B. lentus, B. schlegelii, B. pumilus and B. subtilis. Remaining two were Gram negative and identified as Enterobacter cloacae sub. sp. dissolvens and Pantoea agglomerans. Molecular analysis was conducted on the basis of 16S rDNA sequence to confirm three isolates. Culture and sensitivity test was carried out to screen out the antibiotic sensitivity where streptomycin (S-10), polymyxin (PB-300) and gentamicin (CN-120) antibiotics were found to be effective against all bacterial isolates. The culture and sensitivity test reflected the feasibility to control or eliminate the contaminant bacteria during in vitro culture of plant which is very much required in the commercial tissue culture production.Plant Tissue Cult. & Biotech. 28(1): 99-108, 2018 (June)

scholarly journals In vitro production of capsaicin through plant tissue culture

2017 ◽  
pp. 24-33
Author(s):  
Swetnisha, Ajitabh Bora, H.K. Gogoi, P.S. Raju
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Production ◽  
Agricultural Produce ◽  
Medicinal Properties ◽  
Method Of Production ◽  
Culture Strategies ◽  
Vitro Production

Capsaicin, a secondary metabolite produced in capsicum, is in high demand in pharmaceutical industry because of its various medicinal properties. Currently, the supply of capsaicin depends upon its extraction from capsicum fruits. This limits the production of capsaicin as it depends upon agricultural produce. The current review has compiled information from various literature published on chemistry and importance of capsaicin along with its method of production. It also reviews the process of in vitro production of capsaicin through plant tissue culture, strategies of increasing capsaicin accumulation and its advantages over extraction from fruits and artificial synthesis.

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