scholarly journals In vitro growth of Brassocattleya orchid hybrid in different concentrations of KNO3, NH4NO3 and benzylaminopurine

2011 ◽  
Vol 29 (3) ◽  
pp. 359-363 ◽  
Author(s):  
Jean C Cardoso ◽  
Elizabeth O Ono
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Ornamental Plants ◽  
Dry Weight ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Mass Propagation ◽  
Mineral Salts

One of the most important applications of plant tissue culture is mass propagation of ornamental plants. This experiment evaluated the effect of different concentrations of NH4NO3 and KNO3 and BAP on the in vitro growth of orchid hybrid Brassocattleya 'Pastoral'. Seedlings of this orchid hybrid were used as explants and cultivated in medium with mineral salts and vitamins from the MS medium (Murashige & Skoog, 1962), with the macronutrients P, Ca and Mg reduced by half, and with an addition of 25 g L-1 of sucrose, 0.1 g L-1 of myo-inositol and 1.5 g L-1 of activated charcoal. Agar-agar was added (6.5 g L-1) and the pH was adjusted to 5.8. As treatments, four concentrations of the NH4NO3 and KNO3 (2x; 1x; ½ and ¼ MS medium) and three concentrations of BAP (0.0; 0.5 and 1.0 mg L-1) were assayed. The multiplication, growth in height, fresh and dry weight and sugar level in dry weight of sprouts were evaluated. There occurred a higher growth in height with 0.25x NH4NO3 and KNO3 salts concentrations of MS medium and higher rate of multiplication with combination of NH4NO3 and KNO3 reduced by half of the MS medium concentration and 1.0 mg L-1 BAP.

scholarly journals PLANT TISSUE CULTURE: A TOOL TO CONSERVE BIODIVERSITY THROUGH RAPID IN VITRO REGENERATION OF DIVERSE PLANT SPECIES

2017 ◽  
pp. 132-139
Author(s):  
Nirali Vora
Keyword(s):  
Tissue Culture ◽  
Plant Species ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Regeneration ◽  
Ornamental Plants ◽  
Cost Effective ◽  
Human Consumption ◽  
Natural Forests

Biodiversity is declining with the loss of natural forests across the world. Plant tissue culture is an important biotechnological tool to raise large number of plant species in short spa of time. However, commercial tissue culture laboratories are working on raising plantlets important for human consumption only; which mainly include fruit crops, ornamental plants, timber-yielding forest trees and medicinal plants. There is an urgent need of raising all the different plant species rapidly through tissue culture. Through cultivation of these high yielding and disease-free crops in the forests for consumption of all other species of fauna; conservation of biodiversity can be achieved. However, as tissue culture plantlets are costlier than conventionally raised plants, despite of its advantages its utility is limited. To reduce cost of an important fruit crop, banana during its in vitro regeneration, cost-effective alternatives are proposed.

scholarly journals INFLUENCE OF CYTOKININS AND SUCROSE CONCENTRATIONS ON BULBLET CHARACTERS OF IN VITRO HIPPEASTRUM HYBRIDUM

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
Al-Amery & Salman
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Dry Weight ◽  
Culture Technique ◽  
College Of Agriculture ◽  
Tissue Culture Technique

The First Part of this study was conducted at the Plant Tissue Culture Lab at the College of Science, University of Nahrain from October 2014 to February,2015. and completed at the Plant Tissue Culture Lab at the College of Agriculture, University of Baghdad From February 2015 to September 2015. Examine the possibility of using the tissue culture technique in the propagation of Hippeastrum  hybridum.plantlets were resulted from leaves induced plant lets moved to new MS media supplemented with BA and Kin at 2.0, 1.0, 0.5, 0.0 mg.liter-1 individually or in combination and with or without NAA at 0.5, 0.3, 0.1, 0.0 mg.liter-1 to enhance shoot proliferation. Transferred shoot  from the best proliferation- enhance to stage bulbs formation, MS media supplemented with BA at 6.0, 3.0, 1.5, 0.0 0  mg.liter-1 in addition to sucrose at  90, 60, 300 g.liter-1 with the present of NAA at 0.1 mg.liter-1 to increase bulbs formation, weight, and diameter .Result showed that the best  shoot proliferation media was MS supplemented with 1.0 mg.liter-1 BA and 0.3 mg.liter-1 NAA which resulted in 8.30 shoots.plant-1. As for bulbs formation, the results exhibited that MS media supplemented with  6.0  mg.liter-1 BA and 90 g.liter-1 sucrose with the existence of 0.10 mg.liter-1 NAA gave the highest bulbs formation percentage, diameter, and both fresh and dry weight which were 3 bulbs.explant-1, 0.98 cm, and 1.04 and 0.25 g, respectively.

Media derived from brown seaweeds Cystoseira myriophylloides and Fucus spiralis for in vitro plant tissue culture

2016 ◽  
Vol 128 (2) ◽  
pp. 437-446 ◽  
Author(s):  
Siham Esserti ◽  
Mohamed Faize ◽  
Lalla Aicha Rifai ◽  
Amal Smaili ◽  
Malika Belfaiza ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Brown Seaweeds ◽  
Fucus Spiralis ◽  
In Vitro Plant

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals In vitro plant tissue culture: means for production of biological active compounds

Planta ◽  
2018 ◽  
Vol 248 (1) ◽  
pp. 1-18 ◽  
Author(s):  
Claudia A. Espinosa-Leal ◽  
César A. Puente-Garza ◽  
Silverio García-Lara
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Compounds ◽  
In Vitro Plant

scholarly journals Endogenous Bacterial Contamination of Plant Tissue Culture Materials: Identification and Control Strategy

2018 ◽  
Vol 28 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Mohammad Ali ◽  
Shefali Boonerjee ◽  
Mohammad Nurul Islam ◽  
Mihir Lal Saha ◽  
M Imdadul Hoque ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacterial Contamination ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Bacterial Isolates ◽  
Culture And Sensitivity ◽  
And Control

The endogenous bacterial contamination of plant tissue culture materials and their possible control was studied. Nine bacterial isolates were isolated from the contaminated tissue culture materials viz. potato and tea. On the basis of morphology and biochemical characters of nine isolates, seven were identified as Gram positive belonging to Bacillus alcalophilus, B. circulans, B. infantis, B. lentus, B. schlegelii, B. pumilus and B. subtilis. Remaining two were Gram negative and identified as Enterobacter cloacae sub. sp. dissolvens and Pantoea agglomerans. Molecular analysis was conducted on the basis of 16S rDNA sequence to confirm three isolates. Culture and sensitivity test was carried out to screen out the antibiotic sensitivity where streptomycin (S-10), polymyxin (PB-300) and gentamicin (CN-120) antibiotics were found to be effective against all bacterial isolates. The culture and sensitivity test reflected the feasibility to control or eliminate the contaminant bacteria during in vitro culture of plant which is very much required in the commercial tissue culture production.Plant Tissue Cult. & Biotech. 28(1): 99-108, 2018 (June)

scholarly journals In vitro production of capsaicin through plant tissue culture

2017 ◽  
pp. 24-33
Author(s):  
Swetnisha, Ajitabh Bora, H.K. Gogoi, P.S. Raju
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
In Vitro Production ◽  
Agricultural Produce ◽  
Medicinal Properties ◽  
Method Of Production ◽  
Culture Strategies ◽  
Vitro Production

Capsaicin, a secondary metabolite produced in capsicum, is in high demand in pharmaceutical industry because of its various medicinal properties. Currently, the supply of capsaicin depends upon its extraction from capsicum fruits. This limits the production of capsaicin as it depends upon agricultural produce. The current review has compiled information from various literature published on chemistry and importance of capsaicin along with its method of production. It also reviews the process of in vitro production of capsaicin through plant tissue culture, strategies of increasing capsaicin accumulation and its advantages over extraction from fruits and artificial synthesis.

scholarly journals Effect of Plant Preservative Mixture Ppmtm on the Shoot Regeneration of Watercress (Nasturtium Officinale)

2017 ◽  
Vol 5 (2) ◽  
pp. 187
Author(s):  
Hadar Saeed Faizy ◽  
Sami Rishak AL-Zubaydi ◽  
Muraleedharan Nair
Keyword(s):  
Tissue Culture ◽  
Shoot Regeneration ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Dry Weight ◽  
Single Node ◽  
Nasturtium Officinale ◽  
Apical Buds ◽  
Pair Number ◽  
Conventional Antibiotics

Plant Preservative Mixture™ (PPM), a relatively new broad-spectrum preservative and biocide for use in plant tissue culture, was evaluated as an alternative to the use of conventional antibiotics and fungicides in plant tissue culture. Culture inoculated in MS media supplemented with PPM (1.5 ml/l) was the effective concentration which gave the best values. The top values were recorded for all studied characters using apical buds compared with lateral buds. The combination between apical buds and (1.5 ml/l ) PPM concentration showed the superior values of all studied parameters( 33.25%,19.40%, 14.46%,20.53% and 79.46%)(60.55%,39.80%,20.97%,45.33% and 54.44%) and (31.20%, 20. 06%, 12.33%, 35.13% and 81.06 %) for contamination, %bacterial contamination, % offungi contamination, dead explants% and survival explants% respectively.  Different concentrations of PPM (0, 0.5, 1.0, 1.5, 2.0 and 2.5 ml/l) were tested using single node and apex explants of watercress (Nasturtium officinale). PPM at (1.5ml/l)  had significant effect on the studied characters; shoots height, shoots number, leaf pairs number, fresh and dry weight which they reaches (4.16, 4.62, 42.00,0.524 and 0.063 g, respectively ). Apex bud explants showed the greatest effect on shoots height shoots number, leaf pairs number, fresh and dry weight and their values were 3.52, 32.02, 3.38, 0.405 and 0.036 g, respectively. The best parameter were recorded on MS media supplemented with PPM at (1.5ml/l) with apex buds explant  (4.55, 5.34, 46.28, 0.570 and 0.085, respectively) for shoots height, shoots  number, leaf pair number, fresh and dry weight. Current study aimed to determine the best concentration of PPM for limiting the contamination of watercress and micro shoot regeneration.

scholarly journals INFLUENCE OF STRESS ON SECONDARY METABOLITES PRODUCTION FROM CALLUS OF MORINGA OLEIFERA IN VITRO

2017 ◽  
Vol 48 (4) ◽  
Author(s):  
Ibrahim & Ameen
Keyword(s):  
Tissue Culture ◽  
Moringa Oleifera ◽  
Active Material ◽  
Dry Weight ◽  
Ms Medium ◽  
Poly Ethylene Glycol ◽  
College Of Agriculture ◽  
Poly Ethylene ◽  
Metabolites Production

An experiment was conducted to study the effect of sucrose, poly ethylene glycol (PEG) on hypocotyl induced callus of Moringa oleifera at the plant tissue culture lab.- College of Agriculture– University of Baghdad from February 2015 to May 2016. Sucrose concentrations were 30, 60, and 90, 120 g .L -1 and PEG 0, 25, 50 and 100 g .L -1 added to MS medium supplemented with 2.0 mg .L -1 of 2, 4-D and 0.1 mg .L -1 of NAA. MS medium supplemented with120 g .L -1 of sucrose gave the best amount of Zeatin, Quercetin and Kaempferol reached to 103.4, 1324.6 and 966.5 µg. g dry weight of callus-1 respectively. The concentrations of active compound increasing with adding PEG, MS medium supplemented with 100 g .L -1 PEG gave the highest value of Zeatin, Quercetin and Kaempferol which recorded 92.01, 3528.0 and 931.0 µg. g dry weight of callus-1 respectively. We found that we could increase the production of active material from callus that induced from explant by exposure the callus to several stress and then could separate the pure active material and used it as a drug in medicine.

scholarly journals Sodium Dichloroisocyanurate: An eco-friendly chemical alternative for media autoclaving and explant sterilisation in plant tissue culture

2021 ◽  
Vol 12 (1) ◽  
pp. 107-112
Author(s):  
Simran Chandrahas Shetty ◽  
Narasimhan S
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Active Agent ◽  
Electrical Energy ◽  
Life Forms ◽  
Water Soluble ◽  
Viable Alternative ◽  
Sodium Dichloroisocyanurate

Autoclaving nutrient media is still considered as the optimum mode of sterilisation in plant cell and tissue culture. During the process steam under high pressure is maintained at 120 degrees Celsius, 15 psi for 15-20 minutes in a chamber, optimised to kill all possible microbial life forms. But the disadvantages related to the process of autoclaving are plentiful. They are, decrease in the media pH, salt precipitation, agar depolymerisation, carbohydrate hydrolysis, volatile obliteration and necessity of the infrastructure investment. Requirements of additional resources (time, human resources, electrical energy) have forced the lookout for a more viable alternative, that is, chemical sterilisation. The use of Sodium dichloroisocyanurate (NaDCC) is a useful alternative for media and explant sterilisation. NaDCC is stable, water-soluble, non-toxic and easy to use at room temperature, does not have any environmental hazards and is not phytotoxic. The use of NaDCC as a disinfectant has been documented well concerning water sterilisation, surface sterilisation and also as a broad spectrum disinfecting agent. Disinfecting property of NaDCC is due to the hydrolytic release of chlorine, and this can be utilised for sterilisation of media and explants in plant tissue culture. NaDCC is a useful alternative for autoclaving at a concentration range of 0.05 to 1.0 g/l. However, only a few reports are available for its use as a sterilising agent for media and explants for in vitro cultures of plants. This paper discusses and reviews the possibility of establishing NaDCC as an active agent for explant sterilisation and as a viable alternative to medium sterilisation through autoclaving.

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