scholarly journals IN VITRO PROPAGATION OF MORINGA OLEIFERA

2017 ◽  
Vol 48 (4) ◽  
Author(s):  
Ibrahim & Ameen
Keyword(s):  
In Vitro Propagation ◽  
Moringa Oleifera ◽  
Nodal Segment ◽  
Ms Medium ◽  
College Of Agriculture ◽  
Single Node ◽  
Soil Mixture ◽  
Survival Percentage ◽  
Half Strength

This research was conducted at the plant tissue culture lab. - College of Agriculture – University of Baghdad from February 2015 to May 2016. The Study was aimed to investigate the in vitro propagation of Moringa oleifera, by inoculation a single nodal segment in MS medium supplemented with different concentrations of plant growth regulators. The best responded of single node reached to 90% was achieved with MS medium supplemented with 1 mg. L-1 BA and 0.2 mg. L-1 IAA. At Multiplication stage results showed that MS medium supplemented with 2 mg. L-1 of BA with 0.1 mg. L-1 IAA increased numbers of shoot comparing with the Kin; (6.40 shoot /exp.) While the treatment of MS medium supplemented with 0.2 mg. L-1 IAA without BA gave the best shoots length which reached 4.15 cm. In rooting stage, shoots have been cultured in MS medium supplemented with different concentrations of Auxins. Results showed that MS medium at half strength supplemented with 1 mg. L-1 of IBA and 1.5 mg. L-1 IAA significantly increased the number of roots per shoot, roots length up to (7.2 roots/shoot and 6.14 cm) respectively. The survival percentage of plantlets was 70% when they planted in a composed consisted of 3:1 (v:v) peatmoss: soil mixture. We found that BA increased numbers of shoot comparing with the Kin and MS medium at half strength was the best in rooting plantlet comparing with MS medium at full strength.

scholarly journals Effect of explant type on the rooting and acclimatization of Dianthus serotinus Waldst. & Kit.

2014 ◽  
pp. 125-136
Author(s):  
Marija Markovic ◽  
Mihailo Grbic ◽  
Dragana Skocajic ◽  
Matilda Djukic
Keyword(s):  
Ms Medium ◽  
Explant Type ◽  
Single Node ◽  
In Vitro Plantlets ◽  
Half Strength ◽  
Terminal Buds

The effect of the concentration of MS salts and explant type on D. serotinus rooting and acclimatization was investigated in order to optimize a protocol for the micropropagation of this species. The obtained results showed that explant type as well as the concentration of MS salts had a significant effect on rooting, and the highest rooting rate (85-86,7%) was achieved when culturing single-node cuttings and terminal buds on a half-strength MS medium supplemented with 0,5 mgL-1 NAA. Nevertheless, mean number of roots per explant was higher on the MS media (15,3-18,6) than on the half-strength MS media (11,8-13,4). The best acclimatization rate was obtained in a 4:1 mixture of peat and sand (83,3-86,7%). The explant type from which in vitro plantlets developed had no effect on the acclimatization rate.

scholarly journals Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (Aegle marmelos L.)

1970 ◽  
Vol 44 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Ranjoy Das ◽  
M Faruk Hasan ◽  
Harunar Rashid ◽  
Motiur Rahman
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Nodal Explant ◽  
Aegle Marmelos ◽  
Half Strength ◽  
Subsequent Regeneration

This study reports on an improved protocol for callus induction and subsequent regeneration from nodal segment of wood apple (Aegle marmelos L.) Creamish friable competent callus was achieved from nodal segments on MS medium augmented with 4.0 mg1-1 2,4-D within two weeks of inoculation. The callus produced large number of shoots when cultured on MS medium fortified with 2.0 mgl-1 BAP+0.1 mgl-1 NAA within ten days of culture. In vitro raised shoots were rooted on half strength MS medium enriched with 1.0 mgl-1 IBA within fifteen days of culture. The rooted plantlets were successfully established with 80% survival. Key words: Plant regeneration; Callus induction; Nodal explant; Aegle marmelos. DOI: 10.3329/bjsir.v44i4.4590 Bangladesh J. Sci. Ind. Res. 44(4), 415-420, 2009

scholarly journals In Vitro Propagation of Croton Scabiosus Bedd. (Euphorbiaceae), an Endemic and Vulnerable Tree Species

2013 ◽  
Vol 3 (3) ◽  
pp. 229-241
Author(s):  
B. Ravi Prasad Rao ◽  
S. Salamma
Keyword(s):  
Tree Species ◽  
In Vitro Propagation ◽  
Andhra Pradesh ◽  
Natural Habitat ◽  
Eastern Ghats ◽  
Ms Medium ◽  
Species Population ◽  
Half Strength ◽  
Site Conservation

Croton scabiosus Bedd. (Euphorbiaceae), an endemic tree species of Southern Eastern Ghats of Andhra Pradesh, India, categorised as ‘Vulnerable’ is attempted for in vitro propagation. Seed pathological problems, poor germination, recurrent fires and tree fellings in the native habitat are the major threats of the species. With an objective to standardise a suitable multiplication protocol to augment the species population in the natural habitat, the present study focus on multiplication of the species through micropropagation. Of the various growth regulators employed in the experimentation, maximum mean number of shoots i.e. 5.37±0.12 was found on MS medium fortified with 0.5mg/l BAP and 2.5mg/l IAA. Maximum mean length of shoots 5.27±0.14 was found on 0.5mg/l BAP+1mg/l IAA. In vitro rooting was found on half strength MS medium fortified with 2.5mg/l IBA (8.92±0.19 mean number of roots with 5.04±0.05 mean length of roots with 90% response). Only 10% of survival rate was observed and the species warrants effective on site conservation methods.

scholarly journals INFLUENCE OF STRESS ON SECONDARY METABOLITES PRODUCTION FROM CALLUS OF MORINGA OLEIFERA IN VITRO

2017 ◽  
Vol 48 (4) ◽  
Author(s):  
Ibrahim & Ameen
Keyword(s):  
Tissue Culture ◽  
Moringa Oleifera ◽  
Active Material ◽  
Dry Weight ◽  
Ms Medium ◽  
Poly Ethylene Glycol ◽  
College Of Agriculture ◽  
Poly Ethylene ◽  
Metabolites Production

An experiment was conducted to study the effect of sucrose, poly ethylene glycol (PEG) on hypocotyl induced callus of Moringa oleifera at the plant tissue culture lab.- College of Agriculture– University of Baghdad from February 2015 to May 2016. Sucrose concentrations were 30, 60, and 90, 120 g .L -1 and PEG 0, 25, 50 and 100 g .L -1 added to MS medium supplemented with 2.0 mg .L -1 of 2, 4-D and 0.1 mg .L -1 of NAA. MS medium supplemented with120 g .L -1 of sucrose gave the best amount of Zeatin, Quercetin and Kaempferol reached to 103.4, 1324.6 and 966.5 µg. g dry weight of callus-1 respectively. The concentrations of active compound increasing with adding PEG, MS medium supplemented with 100 g .L -1 PEG gave the highest value of Zeatin, Quercetin and Kaempferol which recorded 92.01, 3528.0 and 931.0 µg. g dry weight of callus-1 respectively. We found that we could increase the production of active material from callus that induced from explant by exposure the callus to several stress and then could separate the pure active material and used it as a drug in medicine.

scholarly journals n vitro micro propagation of Spilanthes acmella (L.) Murr

2014 ◽  
Vol 8 (1) ◽  
pp. 55-60
Author(s):  
Bushra M. Jaber Alwash ◽  
Ansaam Z. Jassim
Keyword(s):  
In Vitro Propagation ◽  
Indole Acetic Acid ◽  
Ms Medium ◽  
Indole Butyric Acid ◽  
Micro Propagation ◽  
Spilanthes Acmella ◽  
Half Strength ◽  
The Mean ◽  
Initiation Stage

This study was aimed to In vitro propagation of Spilanthes acmella L. Murr. It is a medicinal plant not cultivated in Iraq. Seeds were sterilized and cultured on MS medium. Indole acetic acid IAA, Benzyladenin BA growth regulators’ were used at the initiation stage. The combination between IAA and BA was used in multiplication stage. Indole butyric acid IBA was used for rooting the shoots. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. A node exhibited relatively highest response as compared with apical meristems and leaflets culture. Supplying the culture medium with 1mg/l. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding BA at 0.5, 1.0 or 1.5 and IAA at 0.1 mg/l. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 10, 84, 10.00 respectively. Adding 0.1, 0.5, 1.0 mg/l IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots respectively with mean length of 3.25, 3.80, 3.80 cm respectively. Results of acclimatization stage showed that addition of 1:1 Patmos and loamy soil gave the highest rate of survival 100% after 4 weeks of acclimatization. This study showed the ability of in vitro propagation of Spilanthes acmella (L.) Murr

scholarly journals Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

2021 ◽  
Vol 4 (46) ◽  
pp. 17-17
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Ms Medium ◽  
Organic Additives ◽  
Clonal Micropropagation ◽  
Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

scholarly journals In-vitro Conservation of Bacopa monniera (L.) Wettst.: A Memory Booster Plant

Our Nature ◽  
1970 ◽  
Vol 8 (1) ◽  
pp. 40-47 ◽  
Author(s):  
J. Prabha ◽  
U. Rani ◽  
A. Sen ◽  
R.N. Verma ◽  
A. Batra
Keyword(s):  
Growth Regulators ◽  
High Frequency ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Nodal Segment ◽  
Bud Break ◽  
Ms Medium ◽  
Bacopa Monniera ◽  
In Vitro Shoot Regeneration

A mass in-vitro propagation system of Bacopa monniera (L.) Wettst., a traditional medicinal herb, has been developed. Shoot proliferation and growth were greatly influenced by the months of the year during which the explant were collected. High frequency bud break coupled with maximum number of shoots through nodal segment were found in the month of June and August. Of the different growth regulators tried, for in vitro shoot regeneration, MS medium with BAP (1.0 mg/l) induced maximum number of proliferative shoots (65.00±1.453). Multiplication and elongation of the shoots were obtained after regular sub culturing on the same medium after 15-21 days. Rooting from basal end of the shoots occurred on MS medium with the addition of IBA (0.5 mg/l). After a hardening phase of 4 weeks, almost 95% transplantation was success in the field.DOI: 10.3126/on.v8i1.4311

scholarly journals In vitro propagation of banana

1970 ◽  
Vol 34 (2) ◽  
pp. 269-278
Author(s):  
M Rezaul Karim ◽  
MA Malek ◽  
Sajia Rahman ◽  
M Al-Amin ◽  
M Ruhul Amin
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Room Temperature ◽  
Ms Medium ◽  
Musa Sp ◽  
In Vitro Technique ◽  
Half Strength ◽  
The Mean ◽  
Basal Media

An in vitro technique for plant regeneration using meristem-derived plantlets of banana cv. BARI-l (Musa sp.) has been developed. Highest number of shoot regeneration was noticed on basal media supplemented with 7.5 mgL-1 BAP + 0.5 mgL-1 NAA at 30 days after inoculation (DAI). The mean number of shoots significantly reduced when the concentrations of BAP and NAA in the medium was high. Regenerated shoots were rooted on half strength MS medium containing 0.5 mgL-1 IAA + 0.5 mgL-1 IBA at 30 DAI. In vitro raised plantlets were transferred to poly bags containing ground soil and cowdung mixture (1:1) for acclimatization and hardening in room temperature (28-30°C) and the established plantlets are ready for planting in the field. Key Words: In vitro propagation; banana; Musa sp.DOI: 10.3329/bjar.v34i2.5799Bangladesh J. Agril. Res. 34(2): 269-278, June 2009

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Physalis minima L. - a Perennial Medicinal Herb

2010 ◽  
Vol 44 (4) ◽  
pp. 453-456 ◽  
Author(s):  
Farhana Afroz ◽  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Half Strength ◽  
Shoot Tip Explants

The present study describes a protocol for high frequency plant regeneration of Physalis minima. Shoots were induced by culturing nodal segments and shoot tips from 15 day old seedlings. About 29 and 32 shoots were found to be induced from nodal segment and shoot tip explants, respectively, cultured on MS medium supplemented with 1.0 mg/l BAP. When shoots were subcultured on the fresh medium with same component as mentioned above, the shoots were elongated. Shoots rooted well when they were excised individually and implanted on half-strength MS medium with 0.3 mg/l NAA, where 98% shoots rooted within 12-15 days. In vitro grown plantlets with strong root system were successfully established in normal room temperature for seven days before transplanting in pots where they were reared for three weeks through successive acclimatization. The regenerated plants were successfully transferred to the soil with 90% survival rate. Key words: Physalis minima; Medicinal plant; Shoot proliferation; Micropropagation; Regeneration DOI: 10.3329/bjsir.v44i4.4597 Bangladesh J. Sci. Ind. Res. 44(4), 453-456, 2009

scholarly journals In Vitro Propagation of Miscanthus sinensis

HortScience ◽  
1990 ◽  
Vol 25 (10) ◽  
pp. 1291-1293 ◽  
Author(s):  
N.J. Gawel ◽  
C.D. Robacker ◽  
W.L. Corley
Keyword(s):  
In Vitro Propagation ◽  
Miscanthus Sinensis ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Callus Initiation ◽  
Half Strength ◽  
Modified Ms Medium ◽  
D 20 ◽  
2,4 Dichlorophenoxyacetic Acid

Immature inflorescences of Miscanthus sinensis Andress. `Gracillimus', `Variegatus', and `Zebrinus' were cultured on modified MS medium with 9.0 μm 2,4-D, 20 g sucrose/liter, 2.0 g Gelrite/liter, and 0.75 g MgCl2/liter. Organogenesis was observed 8 to 12 weeks after callus initiation. Shoots were rooted on half-strength MS medium without growth regulators. After rooting, tillers were initiated. When transferred to soil, plants matured to flowering quickly and retained their variegation patterns. Propagation through in vitro tillering is suggested. Chemical name used: 2,4-dichlorophenoxyacetic acid (2,4-D).

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