scholarly journals INFLUENCE OF STRESS ON SECONDARY METABOLITES PRODUCTION FROM CALLUS OF MORINGA OLEIFERA IN VITRO

2017 ◽  
Vol 48 (4) ◽  
Author(s):  
Ibrahim & Ameen
Keyword(s):  
Tissue Culture ◽  
Moringa Oleifera ◽  
Active Material ◽  
Dry Weight ◽  
Ms Medium ◽  
Poly Ethylene Glycol ◽  
College Of Agriculture ◽  
Poly Ethylene ◽  
Metabolites Production

An experiment was conducted to study the effect of sucrose, poly ethylene glycol (PEG) on hypocotyl induced callus of Moringa oleifera at the plant tissue culture lab.- College of Agriculture– University of Baghdad from February 2015 to May 2016. Sucrose concentrations were 30, 60, and 90, 120 g .L -1 and PEG 0, 25, 50 and 100 g .L -1 added to MS medium supplemented with 2.0 mg .L -1 of 2, 4-D and 0.1 mg .L -1 of NAA. MS medium supplemented with120 g .L -1 of sucrose gave the best amount of Zeatin, Quercetin and Kaempferol reached to 103.4, 1324.6 and 966.5 µg. g dry weight of callus-1 respectively. The concentrations of active compound increasing with adding PEG, MS medium supplemented with 100 g .L -1 PEG gave the highest value of Zeatin, Quercetin and Kaempferol which recorded 92.01, 3528.0 and 931.0 µg. g dry weight of callus-1 respectively. We found that we could increase the production of active material from callus that induced from explant by exposure the callus to several stress and then could separate the pure active material and used it as a drug in medicine.

scholarly journals Influence of sucrose and tyrosine on callus induction and morphine and codeine production on Papaver somniferum In vitro

2010 ◽  
Vol 4 (2) ◽  
pp. 45-53
Author(s):  
M . S . Hamad ◽  
I. A. Hamza ◽  
S . A . Al-Muktar
Keyword(s):  
Tissue Culture ◽  
Callus Induction ◽  
Drug Research ◽  
Dry Weight ◽  
Papaver Somniferum ◽  
Research Center ◽  
Control Treatment ◽  
Ms Medium ◽  
College Of Agriculture

A study was conducted at the tissue culture lab. College of Agriculture/ University of Baghdad and Drug Research Center/ Health Minstry during 2006 - 2007. Results indicated that MS medium supplemented with 30 g/l of sucrose gave the highest values (630.3, 61.6) mg of both fresh and dry weight of callus respectively. However, values of both parameters were reduced with increasing sucrose concentrations up to 60, 90, 120 g/l (478.2, 346.5, 217.6) mg respectively. MS medium supplemented with 30 mg/l of tyrosine gave the highest values of callus fresh and dry weight (429.7, 42.6) mg respectively. MS medium supplemented with 30 g/l of sucrose and 30 mg/L tyrosine gave the highest values (688.0, 67.5) mg of both fresh and dry weight callus. MS medium supplemented with 30 mg/l of tyrosine and 90 g/l of sucrose gave the highest values (2.9, 2.8) mg/g of morphine and codeine from fresh callus weight respectively while the lowest values of morphine and codeine (0.5K 0.2) mg/g from fresh callus weight respectively of control treatment .

scholarly journals INFLUNCE OF AUXIN AND POLYAMINEES ON ROOTING OF SHOOTS OF CITRUS VOLKAMERIANA ROOTSTOCK IN VITRO

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
Al- Khazali & Hamad
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Tissue Culture ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Root Number ◽  
College Of Agriculture ◽  
Indole Butyric Acid ◽  
Citrus Volkameriana

This  research  was  conducted  in  the  plant  tissue  culture  Lab. College  of Agriculture / University  of  Baghdad  from  February to  October  2015. The aim  of  the  study  was  investigating  the  influence  of  combinations  of Indole  butyric  acid (IBA) ,  Naphthalene  acetic  acid (NAA) and  polyamine Spermidine (Spd.) on rooting of shoots of  citrus volkameriana rootstock cultured  on 1\2  MS medium. The Results indicated that 1/2 MS medium supplemented  with 1.0 mg L-1 (IBA)  gave the highest  percentage  of  rooting  (67 %) which differed significantly  from the MS medium with free auxin IBA  that gave (22%) while  the  MS medium  supplemented with 0.5 mg L-1  spermidine  gave the highest percentage of rooting (63%) that was not significantly different  than other concentrations of Spd . MS medium supplemented with 1.0 mg L-1 IBA and 0.5 mg L-1 Spd. gave the highest percentage of rooting (83%) and the highest root number / shoot (3.17) and highest length of root (3.15 cm) while the MS medium with free auxin IBA and spd. did not give percentage of rooting (0%) for citrus volkameriana rootstock plantlets . The MS medium supplemented with 1.0 mg L-1 NAA gave the highest percentage of rooting (56%) which differed significantly from MS medium with free auxin NAA that gave (22%)  while MS medium supplemented with 0.5mg L-1 spd. gave the highest percentage of rooting (50%) that was not significantly different from other concentration of Spd . MS medium supplemented with 1.0 mg L-1 NAA and 0.5 mg L-1 Spd. gave the highest percentage of rooting (68%) and the highest root number /shoot (2.5) and highest length of root (2.65 cm) while the MS medium with free auxin NAA and Spd. did not gave percentage of rooting (0%) .

scholarly journals In vitro growth of Brassocattleya orchid hybrid in different concentrations of KNO3, NH4NO3 and benzylaminopurine

2011 ◽  
Vol 29 (3) ◽  
pp. 359-363 ◽  
Author(s):  
Jean C Cardoso ◽  
Elizabeth O Ono
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Ornamental Plants ◽  
Dry Weight ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Mass Propagation ◽  
Mineral Salts

One of the most important applications of plant tissue culture is mass propagation of ornamental plants. This experiment evaluated the effect of different concentrations of NH4NO3 and KNO3 and BAP on the in vitro growth of orchid hybrid Brassocattleya 'Pastoral'. Seedlings of this orchid hybrid were used as explants and cultivated in medium with mineral salts and vitamins from the MS medium (Murashige & Skoog, 1962), with the macronutrients P, Ca and Mg reduced by half, and with an addition of 25 g L-1 of sucrose, 0.1 g L-1 of myo-inositol and 1.5 g L-1 of activated charcoal. Agar-agar was added (6.5 g L-1) and the pH was adjusted to 5.8. As treatments, four concentrations of the NH4NO3 and KNO3 (2x; 1x; ½ and ¼ MS medium) and three concentrations of BAP (0.0; 0.5 and 1.0 mg L-1) were assayed. The multiplication, growth in height, fresh and dry weight and sugar level in dry weight of sprouts were evaluated. There occurred a higher growth in height with 0.25x NH4NO3 and KNO3 salts concentrations of MS medium and higher rate of multiplication with combination of NH4NO3 and KNO3 reduced by half of the MS medium concentration and 1.0 mg L-1 BAP.

scholarly journals INFLUNCE OF GROWTH REGULATORS ON CALLUS INDUCTION OF CITRUS VOLKAMERIANA IN VITRO

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
Al- Khazali & Hamad
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Dry Weight ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
College Of Agriculture ◽  
Citrus Volkameriana ◽  
Dichlorophenoxy Acetic Acid

This  research  was  conducted  in  the  plant  tissue  culture  Lab. College  of Agriculture / University  of  Baghdad  from  February to  October  2015. The aim  of  the  study  was  investigating  the  influences  of  combinations  of  Naphthalene  acetic  acid (NAA) , Thidiazuron (TDZ) Spermidine  (Spd. ) and 2,4-Dichlorophenoxy  acetic  acid (2,4-D) , Benzyl  adenine (BA) on callus  induction  and  adventitous  shoot  regeneration  originated  from  cotyledon  of  Citrus volkameriana  seeds. Seeds  were  disinfested  with 0.1 % of  HgCl2  for 15 minutes. The MS  medium  supplemented  with  (0.0,1.5 , 3.0 ) mg L-1  NAA in combination with (0.0, 0.05, 0.1) mg L-1  TDZ and (0.0, 0.5 ,1.0) mg L-1 Spd. and MS medium supplemented with (0.0, 1.5 , 3.0) mg L-1  2,4-D in combination with (0.0 ,1.0 , 2.0 )  mg L-1  BA and (0.0 ,0.5 , 1.0) mg L-1  Spd. the  interaction between 1.5 mg L-1  NAA and (0.05 , 0.1)  mg L-1  TDZ and the interaction between 3.0 mg L-1  NAA and (0.05 ,0.1) mg L-1  TDZ with all concentrations of Spd.   gave  the  highest  percentage of  callus  induction  100 % . While  the  MS  medium  supplemented  with  3 mg L-1 of  2,4-D in  combination  with  all  concentrations  of  BA  and  spd.  gave  the  highest  percentage 100 % of  callus induction. Results showed that  MS medium supplemented  with 1.5 mg L-1  NAA in combination  with  0.1 mg L-1 TDZ and  1.0 mg L-1 spd.  gave  the  highest  values  of  fresh  and  dry  weight  of  callus  (668.8, 44.59 ) mg  respectively . While  the  MS  medium  supplemented  with  3 mg L-1 2,4-D  in combination with 1.0 mg  L-1  spd.  And 0.0 mg L-1 BA gave  the  highest  values  of  fresh  and  dry  weight  of  callus  (709.2 , 47.28 ) mg  respectively.

scholarly journals IN VITRO PROPAGATION OF MORINGA OLEIFERA

2017 ◽  
Vol 48 (4) ◽  
Author(s):  
Ibrahim & Ameen
Keyword(s):  
In Vitro Propagation ◽  
Moringa Oleifera ◽  
Nodal Segment ◽  
Ms Medium ◽  
College Of Agriculture ◽  
Single Node ◽  
Soil Mixture ◽  
Survival Percentage ◽  
Half Strength

This research was conducted at the plant tissue culture lab. - College of Agriculture – University of Baghdad from February 2015 to May 2016. The Study was aimed to investigate the in vitro propagation of Moringa oleifera, by inoculation a single nodal segment in MS medium supplemented with different concentrations of plant growth regulators. The best responded of single node reached to 90% was achieved with MS medium supplemented with 1 mg. L-1 BA and 0.2 mg. L-1 IAA. At Multiplication stage results showed that MS medium supplemented with 2 mg. L-1 of BA with 0.1 mg. L-1 IAA increased numbers of shoot comparing with the Kin; (6.40 shoot /exp.) While the treatment of MS medium supplemented with 0.2 mg. L-1 IAA without BA gave the best shoots length which reached 4.15 cm. In rooting stage, shoots have been cultured in MS medium supplemented with different concentrations of Auxins. Results showed that MS medium at half strength supplemented with 1 mg. L-1 of IBA and 1.5 mg. L-1 IAA significantly increased the number of roots per shoot, roots length up to (7.2 roots/shoot and 6.14 cm) respectively. The survival percentage of plantlets was 70% when they planted in a composed consisted of 3:1 (v:v) peatmoss: soil mixture. We found that BA increased numbers of shoot comparing with the Kin and MS medium at half strength was the best in rooting plantlet comparing with MS medium at full strength.

scholarly journals In vitro salt tolerance induced secondary metabolites production in Abrus precatorius L.

2021 ◽  
pp. 115-120
Author(s):  
P. Deepa ◽  
K.P. Lafna Farshana
Keyword(s):  
Salt Tolerance ◽  
Tolerance Analysis ◽  
Dry Weight ◽  
Nacl Stress ◽  
Therapeutic Effects ◽  
Ms Medium ◽  
Abrus Precatorius ◽  
Phytochemical Compounds ◽  
Metabolites Production

The white seeded Abrus precatorius L. is an important herbaceous medicinal plant with broad range of therapeutic effects. In the present study, the internode was selected as explant for in vitro salt tolerance analysis. For callus induction, MS medium with different concentrations and combinations of BAP, KIN and IBA were used. Better callus Fresh Weight and Dry Weight observed on MS medium supplemented with BAP 0.5mg/l and KIN 1mg/l. To detect the in vitro salt tolerance potential of calli, NaCl at different concentrations (0, 20, 40, 60, 80 and 100mM) were supplemented on MS+BAP0.5mg/l+KIN1mg/l of which 40mM NaCl induced better callus proliferation. The callus grown without NaCl stress showed the presence of eight phytochemical compounds in GC-MS analysis. While the NaCl stress tolerant callus exhibited the presence of seventeen phytochemical compounds. All these analyzed compounds were with antimicrobial / anti-oxidant properties. The present work will be very much helpful to ameliorate the production of medicinally significant compounds in the pharmaceutical industry.   

scholarly journals INFLUENCE OF CYTOKININS AND SUCROSE CONCENTRATIONS ON BULBLET CHARACTERS OF IN VITRO HIPPEASTRUM HYBRIDUM

2016 ◽  
Vol 47 (6) ◽  
Author(s):  
Al-Amery & Salman
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Dry Weight ◽  
Culture Technique ◽  
College Of Agriculture ◽  
Tissue Culture Technique

The First Part of this study was conducted at the Plant Tissue Culture Lab at the College of Science, University of Nahrain from October 2014 to February,2015. and completed at the Plant Tissue Culture Lab at the College of Agriculture, University of Baghdad From February 2015 to September 2015. Examine the possibility of using the tissue culture technique in the propagation of Hippeastrum  hybridum.plantlets were resulted from leaves induced plant lets moved to new MS media supplemented with BA and Kin at 2.0, 1.0, 0.5, 0.0 mg.liter-1 individually or in combination and with or without NAA at 0.5, 0.3, 0.1, 0.0 mg.liter-1 to enhance shoot proliferation. Transferred shoot  from the best proliferation- enhance to stage bulbs formation, MS media supplemented with BA at 6.0, 3.0, 1.5, 0.0 0  mg.liter-1 in addition to sucrose at  90, 60, 300 g.liter-1 with the present of NAA at 0.1 mg.liter-1 to increase bulbs formation, weight, and diameter .Result showed that the best  shoot proliferation media was MS supplemented with 1.0 mg.liter-1 BA and 0.3 mg.liter-1 NAA which resulted in 8.30 shoots.plant-1. As for bulbs formation, the results exhibited that MS media supplemented with  6.0  mg.liter-1 BA and 90 g.liter-1 sucrose with the existence of 0.10 mg.liter-1 NAA gave the highest bulbs formation percentage, diameter, and both fresh and dry weight which were 3 bulbs.explant-1, 0.98 cm, and 1.04 and 0.25 g, respectively.

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