scholarly journals EVALUATION OF BIOLOGICAL ACTIVITY OF GREENLY SYNTHESIZED SILVER NANOPARTICLES USING ALOE VERA GEL EXTRACT AS ANTIBACTERIAL AGENT IN-VITRO AND IN-VIVO

2020 ◽  
Vol 51 (6) ◽  
pp. 1706-1715
Author(s):  
Ibrahim & et al.
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Methicillin Resistance ◽  
Aloe Vera ◽  
Dilution Method ◽  
Lesion Volume ◽  
Aloe Vera Gel ◽  
Complicated Skin

The present study was carried out to synthesis silver nanoparticles by using Aloe Vera gel and evaluates antibacterial activity in vitro and in vivo. The synthesis and characterization of silver nanoparticles was confirmed by Ultra Violet Visible- spectrophotometer, X-ray diffraction, Fourier Transmission Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy. Reduction of the Ag+ to Ag0 during exposure to the Aloe Vera gel extract was followed by color change of the solution from colorless, yellow to dark brown within 24 hours. It is observed that surface Plasmon resonance peaks of the maximum absorbance of silver-nanoparticles occur at 425 nm, indicating that AgNPs were produced. Later on, using agar well diffusion and tube dilution method against pathogenic methicillin resistance staphylococcus aureus, (MRSA). Pseudomonas. aeruginosa and Escherichia coli, the antimicrobial properties of synthesised AgNPs were investigated. To confirm in-vivo antibacterial activity, after inducing complicated skin and soft tissue infection in mice by injecting S.aureus subcutaneously. 16µg/gm AgNPs were applied skin infected daily for three days. Silver nanoparticles were as succeeded to reduce the lesion volume in infected mice and reduce the acute inflammation symptoms as clindamycin, In conclusion, A new approach can be used to combat serious infections caused by MRSA by Aloe Vera AgNPs.

Evaluation of Isolated Fractions of Aloe vera Gel Materials on Indinavir Pharmacokinetics: In vitro and in vivo Studies

2016 ◽  
Vol 13 (3) ◽  
pp. 471-480 ◽  
Author(s):  
Lonette Wallis ◽  
Maides Malan ◽  
Chrisna Gouws ◽  
Dewald Steyn ◽  
Suria Ellis ◽  
...  
Keyword(s):  
Aloe Vera ◽  
In Vivo Studies ◽  
Aloe Vera Gel

scholarly journals In Vivo Study of the Antibacterial Chitosan/Polyvinyl Alcohol Loaded with Silver Nanoparticle Hydrogel for Wound Healing Applications

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Tan Dat Nguyen ◽  
Thanh Truc Nguyen ◽  
Khanh Loan Ly ◽  
Anh Hien Tran ◽  
Thi Thanh Ngoc Nguyen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Polyvinyl Alcohol ◽  
Healing Rate ◽  
Antibacterial Effects ◽  
Topical Gel ◽  
Significant Difference

Silver nanoparticles have attracted great interests widely in medicine due to its great characteristics of antibacterial activity. In this research, the antibacterial activity and biocompatibility of a topical gel synthesized from polyvinyl alcohol, chitosan, and silver nanoparticles were studied. Hydrogels with different concentrations of silver nanoparticles (15 ppm, 30 ppm, and 60 ppm) were evaluated to compare their antibacterial activity, nanoparticles’ sizes, and in vivo behaviors. The resulted silver nanoparticles in the hydrogel were characterized by TEM showing the nanoparticles’ sizes less than 22 nm. The in vitro results prove that the antibacterial effects of all of the samples are satisfied. However, the in vivo results demonstrate the significant difference among different hydrogels in wound healing, where hydrogel with 30 ppm shows the best healing rate.

scholarly journals Enhancement of the Antibacterial Activity of Silver Nanoparticles against Phytopathogenic BacteriumRalstonia solanacearumby Stabilization

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Juanni Chen ◽  
Shili Li ◽  
Jinxiang Luo ◽  
Rongsheng Wang ◽  
Wei Ding
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Synergistic Effects ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Tween 80 ◽  
Phytopathogenic Bacterium ◽  
Crop Disease

In this paper, the enhanced antibacterial activity of silver nanoparticles (AgNPs) against the phytopathogenic bacteriumRalstonia solanacearumafter stabilization using selected surfactants (SDS, SDBS, TX-100, and Tween 80) was examined, in comparison with silver ion. Tween 80 was found to be the most preferable stabilizer of AgNPs due to the beneficial synergistic effects of the AgNPs and surfactant. However, all the surfactants nearly had no effects on the antibacterial activity of Ag+.In vitro, Tween 80-stabilized AgNPs showed the highest bactericidal activity againstR. solanacearum. Further measurements using TEM, fluorescence microscopy, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that though Ag+and Tween 80-Ag+induced high toxicity, Tween 80-stabilized AgNPs displayed most severe damage when in direct contact with cells, causing mechanistic injury to the cell membrane and strongly modifying and destructing the cellular proteins. Meanwhile,in vivo, the pot experiments data indicated that the control efficiency of Tween 80-stabilized AgNPs on tobacco bacterial wilt was 96.71%, 90.11%, and 84.21%, at 7 days, 14 days, and 21 days, respectively. Based on the results evidencing their advantageous low dosage requirements and strong antimicrobial activity, Tween 80-stabilized AgNPs are a promising antibacterial agent for use in alternative crop disease control approaches.

An in vitro and in vivo toxicologic evaluation of a stabilized aloe vera gel supplement drink in mice

2013 ◽  
Vol 55 ◽  
pp. 363-370 ◽  
Author(s):  
Inder Sehgal ◽  
Wallace D. Winters ◽  
Michael Scott ◽  
Konstantine Kousoulas
Keyword(s):  
Aloe Vera ◽  
Aloe Vera Gel

scholarly journals The Role of Processed Aloe vera Gel in Intestinal Tight Junction: An In Vivo and In Vitro Study

2021 ◽  
Vol 22 (12) ◽  
pp. 6515
Author(s):  
Thu Han Le Phan ◽  
Se Yong Park ◽  
Hyun Jin Jung ◽  
Min Woo Kim ◽  
Eunae Cho ◽  
...  
Keyword(s):  
Tight Junction ◽  
Healthy Lifestyle ◽  
Oral Treatment ◽  
Aloe Vera ◽  
In Vitro Study ◽  
Leaky Gut ◽  
Age Related ◽  
Aloe Vera Gel

Leaky gut is a condition of increased paracellular permeability of the intestine due to compromised tight junction barriers. In recent years, this affliction has drawn the attention of scientists from different fields, as a myriad of studies prosecuted it to be the silent culprit of various immune diseases. Due to various controversies surrounding its culpability in the clinic, approaches to leaky gut are restricted in maintaining a healthy lifestyle, avoiding irritating factors, and practicing alternative medicine, including the consumption of supplements. In the current study, we investigate the tight junction-modulating effects of processed Aloe vera gel (PAG), comprising 5–400-kD polysaccharides as the main components. Our results show that oral treatment of 143 mg/kg PAG daily for 10 days improves the age-related leaky gut condition in old mice, by reducing their individual urinal lactulose/mannitol (L/M) ratio. In concordance with in vivo experiments, PAG treatment at dose 400 μg/mL accelerated the polarization process of Caco-2 monolayers. The underlying mechanism was attributed to enhancement in the expression of intestinal tight junction-associated scaffold protein zonula occludens (ZO)-1 at the translation level. This was induced by activation of the MAPK/ERK signaling pathway, which inhibits the translation repressor 4E-BP1. In conclusion, we propose that consuming PAG as a complementary food has the potential to benefit high-risk patients.

scholarly journals IN VITRO ANTIBACTERIAL EFFICACY OF PLECTRANTHUS AMBOINICUS MEDIATED SILVER NANOPARTICLES AGAINST URINARY TRACT PATHOGENS

2019 ◽  
pp. 153-159
Author(s):  
SREELAKSHMY S ◽  
THANGAPANDIYAN S
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Urinary Tract ◽  
Leaf Extract ◽  
Spherical Shape ◽  
Plate Count ◽  
Dilution Method ◽  
Klebsiella Pneumonia ◽  
Plectranthus Amboinicus

Objective: The objective of the present study is to synthesize green mediated silver nanoparticles (AgNPs) using Plectranthus amboinicus leaf extract and to evaluate its antibacterial activity against selected urinary tract infection (UTI) causing bacteria. Methods: Phytoconstituents present in leaf extract of P. amboinicus (Lour.) Spreng were analyzed by standard qualitative tests. The synthesized AgNPs were characterized by visual inspection, ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and energy dispersive spectroscopy. The antibacterial activity of synthesized AgNPs was examined against the UTI pathogens such as Pseudomonas aeruginosa, Enterobacter sp., Klebsiella pneumonia, and Escherichia coli. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNPs were carried out by broth dilution method and standard plate count method, respectively. Results: Synthesized AgNPs exhibited a spherical shape with an average size of 17.3 nm. The antibacterial activity of AgNPs against tested bacterial pathogens showed a maximum inhibition zone of 22.00±1.00 mm against P. aeruginosa. The bacterial growth inhibition was confirmed by MIC and MBC. Conclusion: P. amboinicus leaf extract mediated AgNPs could be used as an effective antibacterial agent for the management of UTIs. Surface modifications of urinary catheters with AgNPs may prevent the risk of contamination and the associated infections.

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