A COMPARATIVE STUDY OF HEMATOXYLIN & EOSIN, SPECIAL STAINS AND IMMUNOHISTOCHEMISTRY FOR DETECTION OF HELICOBACTER PYLORI IN CASES OF GASTRITIS
Background: The role of Helicobacter Pylori in the pathogenesis of Gastritis-Peptic ulcer syndrome and its association with the development of upper gastrointestinal tract malignancy warrant efcient method for the identication of the bacteria in biopsy specimens. Four methods - Hematoxylin and Eosin, Giemsa, Toluidine Blue and Immunohistochemistry were compared to detect the presence of Helicobacter pylori. The prevalence of H.Pylori in gastric biopsies was also evaluated. Aim Of The Study: The aim is to evaluate the prevalence of H.Pylori in cases of gastritis and compare the efcacy of Hematoxylin and Eosin, Giemsa , Toluidine blue and Immunohistochemistry for the detection of H.pylori in cases of gastritis. Materials And Methods: This Retrospective study was done in the Department of Pathology. A total of 40 cases of gastritis were randomly selected for this study and all the four methods were applied. Results: Helicobacter pylori infection showed an overall prevalence rate of about 75%. When compared with Immunohistochemistry, Sensitivity and specicity of Hematoxylin and Eosin was 66.67% and 100% respectively. Giemsa showed sensitivity of about 86.67% and specicity of about 100%. Toluidine blue showed sensitivity of about 80% and specicity of about 100%. Conclusion: Hence in the present study Giemsa was more reliable and cost effective stain when compared with Hematoxylin & Eosin, Toluidine blue and immunohistochemistry. However, Immunohistochemistry carries the highest level of sensitivity in the detection of Helicobacter Pylori especially when the density of organism is low and in clinically suspected cases of Helicobacter Pylori with negative Giemsa staining