scholarly journals Phage display – a tool for detection and prevention against pathogens. A review

2021 ◽  
Vol 8 (1) ◽  
pp. 23-33
Author(s):  
Barbora Vidová ◽  
Andrej Godány ◽  
Ernest Šturdík
Keyword(s):  
Nucleotide Sequence ◽  
Phage Display ◽  
Genome Sequencing ◽  
Treatment Strategies ◽  
Microbial Pathogens ◽  
Molecular Technique ◽  
Bacterial Detection ◽  
Foreign Proteins ◽  
Sequence Encoding ◽  
Biology Research

In this article are reviewed the promising uses of phage display in the areas such as microbial pathogens detection of and vaccination. Phage display is a molecular technique by which foreign proteins are expressed at the surface of phage particles. Such phages thereby become vehicles for expression that not only carry within them the nucleotide sequence encoding expressed proteins, but have also the capability to replicate. Recent data acquired from genome sequencing and advances in phage biology research have aided the development of phage-derived bacterial detection and treatment strategies.

scholarly journals Primary immune deficiencies with defects in neutrophil function

Hematology ◽  
2016 ◽  
Vol 2016 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Mary C. Dinauer
Keyword(s):  
Fungal Infections ◽  
Leukocyte Adhesion ◽  
Genetic Disorders ◽  
Clinical Manifestations ◽  
Treatment Strategies ◽  
Adaptor Protein ◽  
Invasive Fungal Disease ◽  
Neutrophil Function ◽  
Microbial Pathogens ◽  
Immune Deficiencies

Abstract Immune deficiencies resulting from inherited defects in neutrophil function have revealed important features of the innate immune response. Although sharing an increased susceptibility to bacterial and fungal infections, these disorders each have distinctive features in their clinical manifestations and characteristic microbial pathogens. This review provides an update on several genetic disorders with impaired neutrophil function, their pathogenesis, and treatment strategies. These include chronic granulomatous disease, which results from inactivating mutations in the superoxide-generating nicotinamide dinucleotide phosphate oxidase. Superoxide-derived oxidants play an important role in the control of certain bacterial and fungal species, and also contribute to the regulation of inflammation. Also briefly summarized are updates on leukocyte adhesion deficiency, including the severe periodontal disease characteristic of this disorder, and a new immune deficiency associated with defects in caspase recruitment domain–containing protein 9, an adaptor protein that regulates signaling in neutrophils and other myeloid cells, leading to invasive fungal disease.

The nucleotide sequence encoding bovine opsin

1984 ◽  
Vol 24 (11) ◽  
pp. 1695 ◽  
Author(s):  
W. Baehr ◽  
M.L. Applebury
Keyword(s):  
Nucleotide Sequence ◽  
Sequence Encoding

scholarly journals Setup, Validation, and Quality Control of a Centralized Whole-Genome-Sequencing Laboratory: Lessons Learned

2018 ◽  
Vol 56 (8) ◽  
Author(s):  
Cath Arnold ◽  
Kirstin Edwards ◽  
Meeta Desai ◽  
Steve Platt ◽  
Jonathan Green ◽  
...  
Keyword(s):  
Public Health ◽  
Quality Control ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Food Poisoning ◽  
Drug Susceptibility ◽  
Lessons Learned ◽  
Microbial Pathogens ◽  
Whole Genome ◽  
Whole Genome Analysis

ABSTRACT Routine use of whole-genome analysis for infectious diseases can be used to enlighten various scenarios pertaining to public health, including identification of microbial pathogens, relating individual cases to an outbreak of infectious disease, establishing an association between an outbreak of food poisoning and a specific food vehicle, inferring drug susceptibility, source tracing of contaminants, and study of variations in the genome that affect pathogenicity/virulence. We describe the setup, validation, and ongoing verification of a centralized whole-genome-sequencing (WGS) laboratory to carry out sequencing for these public health functions for the National Infection Services, Public Health England, in the United Kingdom. The performance characteristics and quality control metrics measured during validation and verification of the entire end-to-end process (accuracy, precision, reproducibility, and repeatability) are described and include information regarding the automated pass and release of data to service users without intervention.

Genome sequencing brought Gossypium biology research into a new era

2017 ◽  
Vol 60 (12) ◽  
pp. 1463-1466 ◽  
Author(s):  
Fuguang Li ◽  
Zuoren Yang ◽  
Zhi Wang ◽  
Zhao’en Yang
Keyword(s):  
Genome Sequencing ◽  
New Era ◽  
Biology Research

scholarly journals Mutational landscape of gastric adenocarcinoma in Chinese: Implications for prognosis and therapy

2015 ◽  
Vol 112 (4) ◽  
pp. 1107-1112 ◽  
Author(s):  
Kexin Chen ◽  
Da Yang ◽  
Xiangchun Li ◽  
Baocun Sun ◽  
Fengju Song ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Clonal Evolution ◽  
Treatment Strategies ◽  
The Cancer Genome Atlas ◽  
Whole Genome Sequencing Data ◽  
Whole Genome ◽  
Sequencing Data ◽  
Phylogenetic Tree Analysis ◽  
Primary Tumors

Gastric cancer (GC) is a highly heterogeneous disease. To identify potential clinically actionable therapeutic targets that may inform individualized treatment strategies, we performed whole-exome sequencing on 78 GCs of differing histologies and anatomic locations, as well as whole-genome sequencing on two GC cases, each with three primary tumors and two matching lymph node metastases. The data showed two distinct GC subtypes with either high-clonality (HiC) or low-clonality (LoC). The HiC subtype of intratumoral heterogeneity was associated with older age, TP53 (tumor protein P53) mutation, enriched C > G transition, and significantly shorter survival, whereas the LoC subtype was associated with younger age, ARID1A (AT rich interactive domain 1A) mutation, and significantly longer survival. Phylogenetic tree analysis of whole-genome sequencing data from multiple samples of two patients supported the clonal evolution of GC metastasis and revealed the accumulation of genetic defects that necessitate combination therapeutics. The most recurrently mutated genes, which were validated in a separate cohort of 216 cases by targeted sequencing, were members of the homologous recombination DNA repair, Wnt, and PI3K-ERBB pathways. Notably, the drugable NRG1 (neuregulin-1) and ERBB4 (V-Erb-B2 avian erythroblastic leukemia viral oncogene homolog 4) ligand-receptor pair were mutated in 10% of GC cases. Mutations of the BRCA2 (breast cancer 2, early onset) gene, found in 8% of our cohort and validated in The Cancer Genome Atlas GC cohort, were associated with significantly longer survivals. These data define distinct clinicogenetic forms of GC in the Chinese population that are characterized by specific mutation sets that can be investigated for efficacy of single and combination therapies.

Nucleotide sequence encoding human pancreatic ribonuclease

1994 ◽  
Vol 1218 (3) ◽  
pp. 466-468 ◽  
Author(s):  
Masaharu Seno ◽  
Jun-ichiro Futami ◽  
Megumi Kosaka ◽  
Satimaru Seno ◽  
Hidenori Yamada
Keyword(s):  
Nucleotide Sequence ◽  
Pancreatic Ribonuclease ◽  
Sequence Encoding ◽  
Human Pancreatic Ribonuclease
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