scholarly journals Oxidação de fármacos por cloro e formação de subprodutos em águas naturais

Revista DAE ◽  
2021 ◽  
Vol 70 (234) ◽  
pp. 129-141
Author(s):  
Brígida Prieto de Souza ◽  
Amanda de Vasconcelos Quaresma ◽  
Diego Roberto Souza Lima ◽  
Marys Lene Braga Almeida ◽  
Robson José de Cássia Franco Afonso ◽  
...  
Keyword(s):  
17Β Estradiol

Neste estudo foi avaliada a remoção de sulfametoxazol, diclofenaco e 17β-estradiol em água natural por meio da oxidação com cloro. As concentrações iniciais dos microcontaminantes, bem como o tempo após a aplicação do cloro, apresentaram diferenças significativas na remoção dos compostos de interesse. As águas naturais foram submetidas ao tratamento por clarificação seguido da pós-cloração, e os resultados revelaram que a melhor eficiência de remoção, com dosagem de cloro de 2,0 mg.L-1 foi da ordem de 58%, 54% e 67% para diclofenaco, 17β-estradiol e sulfametoxazol, respectivamente. O aumento da dose de cloro (3,0 mg.L-1), para tempo de contato de 60 min, não resultou em maior remoção dos microcontaminantes (69% para diclofenaco, 49% para 17β-estradiol e 9,4% para sulfametoxazol). A formação de subprodutos foi observada para os microcontaminantes sulfametoxazol e 17β-estradiol, e não evidenciada para o diclofenaco, o qual apresentou um dos maiores valores de remoção por meio da oxidação com o cloro. Palavras-chave: Fármacos. Cloração. Subprodutos de desinfecção. Tratamento de água.

Effects of Hormone Replacement Therapy on Plasma and Tissue Fibrinolytic Activity in a Rat Model of Surgically Induced Menopause

2014 ◽  
Vol 37 (2) ◽  
pp. 85 ◽  
Author(s):  
Ata Topcuoglu ◽  
Mustafa Albayrak ◽  
Hayriye Erman ◽  
Huriye Balci ◽  
Mesut Karakus ◽  
...  
Keyword(s):  
Hormone Replacement Therapy ◽  
Oral Administration ◽  
Replacement Therapy ◽  
Plasminogen Activator ◽  
Brain Tissue ◽  
Fibrinolytic Activity ◽  
Hormone Replacement ◽  
17Β Estradiol ◽  
Surgically Induced ◽  
Pai 1

Purpose: The purpose of this study was to analyze the effects of estrogen deficiency and hormone replacement therapy (HRT) on fibrinolytic activity in a rat mode of surgically-induced menopause. Methods: Twelve-week-old, sexually mature female Sprague-Dawley rats, each weighing 200–250 g, were randomly divided into four groups: (1) sham-operated group, (2) ovariectomy group, (3) ovariectomy group followed by oral administration of daily 17β-estradiol (0.02 mg/kg/day) (E2) + norethisterone acetate (0.01 mg/kg/day), and (4) ovariectomy group followed by oral administration of daily 17β-estradiol (0.01 mg/kg/day) + drospirenone (0.02 mg/kg/day). Tissue plasminogen activator (tPA) antigen, plasminogen activator inhibitor-1 (PAI-1) antigen, and PAI-1/tPA levels were measured as markers of fibrinolysis in plasma and liver and brain tissue. Results: Compared with sham-operated rats, ovariectomized rats showed higher levels of fibrinolytic activity; however, the increased fibrinolytic activity in plasma and liver tissue was significantly reduced by HRT regimens. No change was observed in the levels of fibrinolytic activity in brain tissue. Conclusions: HRT showed beneficial effects by decreasing fibrinolytic activity related to surgically-induced menopause. Short-term HRT treatment was associated with a shift in the procoagulant-anticoagulant balance toward a procoagulant state.

A Surface Plasmon Resonance Based Inhibition Immunoassay for Sensitive and Selective Detection of 17β-Estradiol

2018 ◽  
Author(s):  
Yong Cao ◽  
Mark T. McDermott
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Quantitative Measurement ◽  
Dynamic Range ◽  
Indirect Detection ◽  
Negative Slope ◽  
Chip Surface ◽  
Detection Mechanism ◽  
17Β Estradiol

<div> <div> <div> <p>Quantitative measurement of small-molecule metabolites is now emerging as an effective way to link the metabolite profile to disease state. Surface plasmon resonance (SPR) is a sensing platform that has demonstrated applicability for a large range of biomolecules. However, direct detection of small molecules with SPR challenges the refractive index based detection mechanism. Herein, we utilized an indirect detection format and developed an inhibition immunoassay for the quantitative measurement of 17β-estradiol (E2) using SPR. One competitor, BSA-E2 conjugate, was immobilized to the SPR chip via the reaction between the primary amino group of the conjugate and the succinimide group (NHS) introduced by the formation of a thiol-NHS monolayer on gold surface. Free E2 molecules compete with BSA-E2 on chip surface for binding sites provided by a monoclonal anti-E2 antibody. It was found the binding affinity of the antibody to BSA-E2 conjugate increases with decreasing surface coverage of BSA-E2 conjugate. Under optimal conditions, a sigmoidal calibration curve with a negative slope and a dynamic range from 10 pM to 2 nM was generated. The detection limit of the immunoassay is estimated to be 0.3 pM. Moreover, the immunoassay exhibits high specificity for E2 detection using estrone (E1) as a potential interference.</p></div></div></div>

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