scholarly journals Chitinase-like protein YKL-40 from nasal mucosa as a biomarker of allergic rhinitis

2012 ◽  
Vol 9 (4) ◽  
pp. 13-17
Author(s):  
YU A Tyurin ◽  
E O Sukmanskaya ◽  
S N Kulikov ◽  
R S Fassakhov
Keyword(s):  
Allergic Rhinitis ◽  
Healthy Volunteers ◽  
Nasal Mucosa ◽  
Allergic Response ◽  
Atopic Diseases ◽  
Perennial Allergic Rhinitis

Background. Chitinase-like protein YKL-40 plays an important role in human atopic diseases. The aim of this study was to determine of the level of chitinase-like protein YKL-40 in the secretions of nasal mucosa of patients with chronic allergic rhinitis. Methods. Samples of allergic nasal mucosa were obtained from twelve patients with perennial allergic rhinitis. Measurement of nasal YKL-40 levels was performed with modification in duplicate using commercially available ELISA kits for YKL-40. The amount of nasal eosinophils and neutrophils were also determined. Results. There were significant differences between healthy volunteers and patients with allergic rhinitis for mucosal YKL-40 levels and the amount of nasal eosinophil and neutrophil cells, which have some characteristics closely associated with allergic response. The nasal YKL-40 levels in patients with allergic rhinitis were in tens times more higher than those in controls. Conclusion. Thus, we conclude that the level of chitinase-like protein YKL-40 was upregulated in allergic nasal mucosa compared with normal nasal mucosa, suggesting their roles in the pathogenesis of allergic rhinitis.

scholarly journals Local allergic rhinitis

2014 ◽  
Vol 67 (suppl. 1) ◽  
pp. 49-56
Author(s):  
Maja Buljcik-Cupic ◽  
Dragana Lemez ◽  
Slobodan Savovic ◽  
Ljiljana Jovancevic ◽  
Danijela Dragicevic
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Immunoglobulin E ◽  
Provocation Test ◽  
Allergic Response ◽  
Prick Test ◽  
Local Allergic Rhinitis ◽  
Provocation Tests ◽  
Number Of Patients ◽  
The Impact

Local allergic rhinitis is a localized allergic response of the nasal mucosa to aeroallergens in the absence of atopy with characteristic production of specific local immunoglobulin E antibodies in the nasal mucosa, T helper type 2 cellular infiltration response during the exposure to aeroallergens and positive results via the nasal allergic provocation test with the release of inflammatory mediators (triptase and eosinophil cationic proteins). Even though the prevalence of local allergic rhinitis has been and is still being investigated, a large number of patients with diagnosed non-allergic rhinitis or idiopathic rhinitis are currently classified as having local allergic rhinitis. The causes of local allergic rhinitis are most commonly house dust, dust mites, pollens and many others. Diagnosis of local allergic rhinitis is made using nasal allergen provocation tests when the prick test for standard inhalation allergens and the serum specific immunoglobulin analysis for aeroallergens are negative. The increasing amount of data on localized allergic response in non-atopic patients asks for many answers regarding local allergic rhinitis. These answers can be obtained by a study on the prevalence and incidence in children and adults, the impact of positive family atopy in the development of disease, the impact of associated diseases of the lower respiratory tract and conjunctiva, the effectiveness of drug treatment and the issue of administration of specific immunotherapy.

Interleukin-13 expression in the nasal mucosa of perennial allergic rhinitis.

1995 ◽  
Vol 152 (6) ◽  
pp. 2059-2067 ◽  
Author(s):  
R U Pawankar ◽  
M Okuda ◽  
S Hasegawa ◽  
K Suzuki ◽  
H Yssel ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Perennial Allergic Rhinitis ◽  
Interleukin 13

scholarly journals EPITHELIAL CELLS EXPRESSING TLR-2 LEVEL AND NOSE MUCOSA BIOCENOSIS IN ALLERGIC RHINITIS PATIENTS

2013 ◽  
Vol 10 (6) ◽  
pp. 20-24
Author(s):  
Yu A Tyurin ◽  
A A Sharifullina ◽  
I G Mustafin ◽  
R S Fassakhov
Keyword(s):  
Allergic Rhinitis ◽  
Epithelial Cells ◽  
Healthy Volunteers ◽  
Nasal Mucosa ◽  
Allergic Diseases ◽  
Nasal Lavage ◽  
Control Group ◽  
Healthy Individuals ◽  
Local Immunity

Background. Determination of local epithelial cells expressing TLR2 as an indicator of local immunity in allergic rhinitis (AR) patients with opportunistic species of staphylococci nasal mucosa colonization. Methods. Washed epithelium samples obtained from patients with seasonal AR (n=8) aged 19—42 years, and perennial AR (n=15) aged 19 —45 years, as well as a control group (20 patients) aged 19—45 years without allergic diseases were investigated. Epithelial cells expressing TLR2 receptors were determined by flow cytometry. Results. The level of epithelial cells expressing TLR2 receptor in patients with seasonal AR was almost in 1,9 times, in perennial AR group — in 1,7 times lower then in healthy individuals. In patients with perennial AR S. aureus was obtained in 96,0% (CI: 79,5—99,2), in association with Str. pyogenes in 29% (CI: 14,9—49,2), Neisseria spp. — in 63,0% (CI: 42,7—78,8). Seasonal allergic rhinitis was characterized by association of S. aureus and S. hemolyticus (70,0%, 44,4—85,8). Conclusion. Ratio of epithelial cells positive for TLR2 in nasal lavage from patients with AR was lower than in healthy volunteers. Indicators proportion of epithelial cells expressing TLR2 in nasal lavage in patients with seasonal AR during an exacerbation period was significantly reduced (1,7—1,9 times), in comparison with healthy volunteers. In AR patients with increased density of staphylococci strains in nasal mucosa increased local epithelial cells expressing TLR2 in nasal lavage was established.

scholarly journals Matrix metalloproteinases and their inhibitors in the nasal mucosa of patients with perennial allergic rhinitis

2001 ◽  
Vol 108 (5) ◽  
pp. 791-796 ◽  
Author(s):  
Azhar Shaida ◽  
Guy Kenyon ◽  
Jagdish Devalia ◽  
Robert J. Davies ◽  
Thomas T. MacDonald ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Matrix Metalloproteinases ◽  
Nasal Mucosa ◽  
Perennial Allergic Rhinitis

scholarly journals Population Pharmacokinetics of Ramatroban in Healthy Volunteers and Patients with Bronchial Asthma/Perennial Allergic Rhinitis.

1997 ◽  
Vol 12 (2) ◽  
pp. 121-129
Author(s):  
Takahiko TANIGAWA ◽  
Kazuhito OKUMURA ◽  
Koichi KAWANO ◽  
Takamichi MATSUKI ◽  
Eiji YUKAWA ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Healthy Volunteers ◽  
Population Pharmacokinetics ◽  
Bronchial Asthma ◽  
Perennial Allergic Rhinitis

Increase in epithelial mast cell numbers in the nasal mucosa of patients with perennial allergic rhinitis

1996 ◽  
Vol 110 (10) ◽  
pp. 929-933 ◽  
Author(s):  
A. Slater ◽  
L. A. Smallman ◽  
A. B. Drake-Lee
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cells ◽  
Light Microscopy ◽  
Nasal Mucosa ◽  
Inferior Turbinate ◽  
Control Group ◽  
Perennial Allergic Rhinitis ◽  
Immunohistochemical Technique ◽  
Cell Numbers ◽  
Staining Methods

AbstractThe aim of the study was to compare the numbers and distribution of mast cells in the nasal mucosa of perennial allergic rhinitis (PAR) patients and controls, as demonstrated by different staining methods for light microscopy.Biopsies of inferior turbinate mucosa were taken from 10 patients with PAR and 10 patients undergoing septoplasty or septorhinoplasty (control group). Sections for light microscopy were stained with azure A, chloroacetate esterase and an ABC immunohistochemical technique using antibody to tryptase.Three times more mast cells were found in the epithelium of PAR patients compared to controls using the immunohistochemical technique (p = 0.0074). This method demonstrated considerably more mast cells than the other stains.The increase in epithelial mast cells is consistent with the migration of mast cells seen in seasonal allergic rhinitis, and this may be important in the phenomenon of nasal priming seen after repeated antigen exposure.

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