scholarly journals Immunohistological Study of Infiltrating Cells in Nasal Mucosa and Nasal Lavage Fluid of Perennial Allergic Rhinitis

1997 ◽  
Vol 100 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Naohiro Suzuki ◽  
Seiichirou Nakabayashi ◽  
Shigeru Nakatsuka ◽  
Tomonori Takasaka
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Perennial Allergic Rhinitis ◽  
Infiltrating Cells ◽  
Nasal Lavage Fluid ◽  
Immunohistological Study

scholarly journals Honeysuckle extract relieves ovalbumin-induced allergic rhinitis by inhibiting AR-induced inflammation and autoimmunity

2019 ◽  
Vol 39 (7) ◽  
Author(s):  
Bin Lin ◽  
Bijuan Cai ◽  
Huige Wang
Keyword(s):  
Allergic Rhinitis ◽  
Nasal Mucosa ◽  
Inflammatory Reaction ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Autoimmune Response ◽  
Enzyme Linked Immunosorbent Assay ◽  
Protein Levels ◽  
Nasal Lavage Fluid ◽  
Ifn Γ

Abstract Honeysuckle has antiviral, antioxidative and anti-inflammatory properties. Allergic rhinitis (AR) is induced by immunoglobulin E (IgE)-mediated inflammatory reaction. Our study investigates whether honeysuckle extract (HE) has therapeutic effect on AR. An AR model of mice was established by ovalbumin (OVA). Hematoxylin–Eosin staining was used to assess nasal mucosa damage. Enzyme-linked immunosorbent assay (ELISA) was performed to determine serum histamine, IgE and interleukin (IL)-2, IL-4, IL-17 and interferon-γ (IFN-γ) from nasal lavage fluid. Western blot was carried out to analyze the protein level from nasal mucosa tissue. We found that HE not only decreased nasal rubbing and sneezing in AR mice, but also reduced AR-induced damage to nasal mucosa. Moreover, HE lowered the levels of serum IgE and histamine and inhibited IL-4 and IL-17 levels from AR mice but raised IL-2 and IFN-γ levels in AR-induced nasal lavage fluid. Our results also showed that HE elevated the protein levels of forkhead box P3 (Foxp3) and T-box transcription factor (T-bet) in AR-induced nasal mucosa tissue, whereas it inhibited signal transducer and activator of transcription (STAT) 3 and GATA binding protein 3 (GATA-3) protein levels. By regulating AR-induced inflammatory reaction and autoimmune response, HE also relieved OVA-induced AR. Thus, HE could be used as a potential drug to treat AR.

scholarly journals The Immuno-Modulatory Activities of Pentaherbs Formula on Ovalbumin-Induced Allergic Rhinitis Mice via the Activation of Th1 and Treg Cells and Inhibition of Th2 and Th17 Cells

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 239
Author(s):  
Peiting Li ◽  
Miranda Sin-Man Tsang ◽  
Lea Ling-Yu Kan ◽  
Tianheng Hou ◽  
Sharon Sze-Man Hon ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
T Cell ◽  
Inflammatory Mediators ◽  
Th17 Cells ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Nasal Epithelium ◽  
Tnf Α ◽  
Nasal Lavage Fluid ◽  
Sinonasal Mucosa

Allergic rhinitis (AR) is a highly prevalent allergic disease induced by immunoglobulin (Ig) E-mediated hypersensitivity reaction at the nasal epithelium against inhaled allergens. Previous studies have demonstrated that Pentaherbs formula (PHF), a modified herbal formula comprising five herbal medicines (Flos Lonicerae, Herba Menthae, Cortex Phellodendri, Cortex Moutan and Rhizoma Atractylodis), could suppress various immune effector cells to exert anti-inflammatory and anti-allergic effects in allergic asthma and atopic dermatitis. The present study aimed to further determine the anti-inflammatory activities of PHF in an ovalbumin (OVA)-induced AR BALB/c mouse model. Nasal symptoms such as sneezing and nose rubbing were recorded and the serum total IgE and OVA-specific IgG1, as well as interleukin (IL)-4, IL-5, IL-10, IL-13, chemokines CXCL9 CXCL10, and tumor necrosis factor (TNF)-α concentrations in nasal lavage fluid (NALF) were measured during different treatments. Effects of PHF on the expression of inflammatory mediators in the sinonasal mucosa were quantified using real-time QPCR. PHF was found to suppress allergic symptoms, infiltration of inflammatory cells, and hyperplasia of goblet cells in the nasal epithelium of the OVA-induced AR mice. PHF could reduce OVA-specific IgG1 level in serum, and TNF-α and IL-10 in nasal lavage fluid (NALF), significantly up-regulate the splenic regulatory T (Treg) cell level, increase the Type 1 helper T cell (Th1)/Type 2 helper T cell (Th2) ratio, and reduce the Th17 cells (all p < 0.05). PHF could also alleviate in situ inflammation in sinonasal mucosa of OVA-induced AR mice. In conclusion, oral treatment of PHF showed immuno-modulatory activities in the OVA-induced AR mice by regulating the splenic T cell population to suppress the nasal allergy symptoms and modulating inflammatory mediators, implicating that PHF could be a therapeutic strategy for allergic rhinitis.

Interleukin-5 Gene Expression in Nasal Mucosa and Changes in Amount of Interleukin-5 in Nasal Lavage Fluid After Antigen Challenge

1994 ◽  
Vol 114 (2) ◽  
pp. 203-208 ◽  
Author(s):  
Nobuhisa Terada ◽  
Akiyoshi Konno ◽  
Setsuya Fukuda ◽  
Tetsuji Yamashita ◽  
Koji Shirotori ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nasal Mucosa ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Antigen Challenge ◽  
Interleukin 5 ◽  
Nasal Lavage Fluid

Ragweed-Specific IgA in Nasal Lavage Fluid of Ragweed-Sensitive Allergic Rhinitis Patients: Increase during the Pollen Season

1991 ◽  
Vol 94 (1-4) ◽  
pp. 275-277 ◽  
Author(s):  
C.E. Reed ◽  
M. Bubak ◽  
S. Dunnette ◽  
J. Blomgren ◽  
M. Pfenning ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Pollen Season ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Nasal Lavage Fluid

scholarly journals Nasal Challenge with Ragweed Pollen Extract (RWPE) Increases the Level of Fortilin in Nasal Lavage Fluid from Subjects with Allergic Rhinitis

2016 ◽  
Vol 137 (2) ◽  
pp. AB94
Author(s):  
Julia W. Tripple ◽  
Koa Hosoki ◽  
Istvan Boldogh ◽  
David Rogers Redding ◽  
Sanjiv Sur ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Lavage Fluid ◽  
Ragweed Pollen ◽  
Nasal Lavage ◽  
Pollen Extract ◽  
Nasal Challenge ◽  
Nasal Lavage Fluid

scholarly journals Inflammatory mediators in nasal lavage among school-age children from urban and rural areas in São Paulo, Brazil

2004 ◽  
Vol 122 (5) ◽  
pp. 204-207 ◽  
Author(s):  
Clóvis Eduardo Santos Galvão ◽  
Paulo Hilário Nascimento Saldiva ◽  
Jorge Elias Kalil Filho ◽  
Fábio Fernandes Morato Castro
Keyword(s):  
Urban Area ◽  
Rural Area ◽  
Nasal Mucosa ◽  
Eosinophil Cationic Protein ◽  
Lavage Fluid ◽  
Nasal Lavage ◽  
Rural Children ◽  
Cationic Protein ◽  
Nasal Airways ◽  
Nasal Lavage Fluid

CONTEXT: Some studies have shown that inflammatory processes in the nasal air passages may reflect or affect those in the lower airways. We decided to indirectly assess the inflammatory status of the nasal airways in two groups of children with different sensitization rates to aeroallergens. OBJECTIVE: To compare the inflammatory activity in the nasal airways, through the determination of mediators in nasal lavage fluid in two distinct populations. TYPE OF STUDY: Cross-sectional study. SETTING: Two public elementary schools, one in an urban setting and the other in a rural setting of the State of São Paulo, Brazil. METHODS: Two groups of 40 elementary school children with different sensitization rates to aeroallergens were formed. Samples of nasal lavage fluid were assessed for eosinophil cationic protein (ECP) and tryptase. Non-parametric tests were used for statistical analysis. RESULTS: Significantly higher levels of ECP were observed among students living in the urban area than those in the rural area (p < 0.05). No significant difference in the tryptase levels was observed. Also, the urban children who were sensitized to aeroallergens presented higher levels of ECP in nasal mucosa than the non-sensitized children, while this difference was not observed among the rural children. DISCUSSION: The lack of mast cell activity and increased eosinophil degranulation revealed a chronic inflammatory state in the nasal air passages. The higher eosinophil activity in the urban area, coinciding with higher sensitization to aeroallergens, suggests that there must be some factors in the urban area that can modulate airway inflammation by influencing the activation of inflammatory cells. CONCLUSION: Our findings showed that there was no difference in the concentrations of tryptase in nasal lavage fluids between the two studied groups. However, the children from the urban area presented with higher concentrations of eosinophil cationic protein than did those from the rural area. Also, the urban children who were sensitized to aeroallergens presented with greater concentrations of eosinophil cationic protein in nasal mucosa than the non-sensitized children, while this difference was not observed among the rural children.

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