Genetic Contribution of Introduced Varieties to Wheat Breeding in China Evaluated Using SSR Markers

2009 ◽  
Vol 35 (5) ◽  
pp. 778-785 ◽  
Author(s):  
Xiao-Jun LI ◽  
Xin XU ◽  
Wei-Hua LIU ◽  
Xiu-Quan LI ◽  
Xin-Ming YANG ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Wheat Breeding ◽  
Genetic Contribution

scholarly journals Characterization of EST‑SSR markers in bread wheat EST related to drought tolerance and functional analysis of SSR‑containing unigenes

2020 ◽  
pp. 1-12
Author(s):  
Laila Dabab Nahas ◽  
Alsamman M. Alsamman ◽  
Aladdin Hamwieh ◽  
Naim Al-Husein ◽  
Ghinwa Lababidi
Keyword(s):  
Drought Tolerance ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Web Sites ◽  
Wheat Breeding ◽  
Genic Ssr ◽  
Drought Tolerant ◽  
Ssr Loci ◽  
Weather Changes

Bread wheat (Triticum aestivum) is an important staple food around the world. The enormous volume of the genome of wheat makes it quite slow to progress in traditional scientific research. On the other hand, incessant databases and suitable tools on web sites make progress in wheat research quicker and easier. Drought is a major abiotic stress in accordance with weather changes and accelerated increase in drylands. In this study, 9077 ESTs related to drought tolerance in hexaploid wheat were downloaded from NCBI and assembled into 12062 contigs and 4141 singletons. It was found that trinucleotide had the highest frequency 64.71%. Moreover, 53.80% of SSRs found in coding regions in respect of ORFs. The highest amino acids found for tri-and hexanucleotides were Arginine. In addition, 81% of SSR-containing unigenes had one chromosome location and the highest number of loci was found in chromosomes 1B (69). The distribution of genic SSR loci among the 21 wheat chromosomes, the three subgenomes, and the seven homoeologous groups of wheat chromosomes was significant, with P<0.01 indicating a non-random distribution. Functional annotation and characterization of SSR-containing unigenes have been performed. Eighty-six sequences were identified and sorted into 25 putative TF families and establish 166 pathways using KEGG. Primer-BLAST was used to predict the polymorphism, which was 39% of the 63 primer pairs of SSR markers. Our current study attempts to help farmers in wheat breeding programs to have drought-tolerant accessions, particularly in developing countries

scholarly journals Molecular Mapping of a Novel Lesion Mimic Gene (lm4) Associated with Enhanced Resistance to Stripe Rust in Bread Wheat

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Mei Du ◽  
Chihong Zhang ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Theoretical Support ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background: Lesion mimics (LMs) are disease-like symptoms that occur randomly on plant green leaves in the absence of pathogens. A previous study showed that LMs are related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can substantially reduce the quality and yield of crops. The development of resistant cultivars is an economical and environmentally friendly way to enhance the adaptability and yield stability of crops instead of the use of fungicide applications. Results: In this study, a novel LM gene affording Pst resistance was identified and mapped with molecular markers developed for marker-assisted selection (MAS)-based wheat breeding. In this study, a novel LM gene named lm4, which is closely linked (8.06 cM) to SSR markers Xgwm210 and Xgwm455, was identified by using a Yanzhan 1/Neixiang 188 RIL population. The genetic distance of lm4 was then narrowed such that it was flanked by SSR markers with 0.51 cM and 0.77 cM intervals. Two SSR markers, lm4_01_cib and lm4_02_cib, were developed based on the content in the Chinese Spring genome database and wheat 660K SNP results; these markers can be used to conduct MAS of LMs in wheat. The results also showed that lm4 significantly improved the resistance of stripe rust in wheat. Conclusions: Therefore, lm4 is associated with stripe rust resistance, which may provide theoretical support for future crop disease-resistance breeding and for understanding the plant apoptosis mechanism.

Distribution of DArT, AFLP, and SSR markers in a genetic linkage map of a doubled-haploid hexaploid wheat population

Genome ◽  
2006 ◽  
Vol 49 (5) ◽  
pp. 545-555 ◽  
Author(s):  
Kassa Semagn ◽  
Åsmund Bjørnstad ◽  
Helge Skinnes ◽  
Anne Guri Marøy ◽  
Yalew Tarkegne ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Segregation Distortion ◽  
Doubled Haploid ◽  
Genetic Linkage ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Leaf Segment ◽  
Dart Markers ◽  
Head Blight ◽  
Diversity Arrays Technology

A genetic linkage mapping study was conducted in 93 doubled-haploid lines derived from a cross between Triticum aestivum L. em. Thell 'Arina' and a Norwegian spring wheat breeding line, NK93604, using diversity arrays technology (DArT), amplified fragment length polymorphism (AFLP), and simple sequence repeat (SSR) markers. The objective of this study was to understand the distribution, redundancy, and segregation distortion of DArT markers in comparison with AFLP and SSR markers. The map contains a total of 624 markers with 189 DArTs, 165 AFLPs and 270 SSRs, and spans 2595.5 cM. All 3 marker types showed significant (p < 0.01) segregation distortion, but it was higher for AFLPs (24.2%) and SSRs (22.6%) than for DArTs (13.8%). The overall segregation distortion was 20.4%. DArTs showed the highest frequency of clustering (27.0%) at < 0.5 cM intervals between consecutive markers, which is 3 and 15 times higher than SSRs (8.9%) and AFLPs (1.8%), respectively. This high proportion of clustering of DArT markers may be indicative of gene-rich regions and (or) the result of inclusion of redundant clones in the genomic representations, which was supported by the presence of very high correlation coefficients (r > 0.98) and multicollinearity among the clustered markers. The present study is the first to compare the utility of DArT with AFLP and SSR markers, and the present map has been successfully used to identify novel QTLs for resistance to Fusarium head blight and powdery mildew and for anther extrusion, leaf segment incubation, and latency.Key words: 'Arina', diversity arrays technology, double haploid, genetic map, marker clustering, microsatellite.

Genetic contribution of foreign germplasm to elite Chinese soybean (Glycine max) cultivars revealed by SSR markers

2006 ◽  
Vol 51 (9) ◽  
pp. 1078-1084 ◽  
Author(s):  
Jun Qin ◽  
Weiyuan Chen ◽  
Rongxia Guan ◽  
Chengxi Jiang ◽  
Yinghui Li ◽  
...  
Keyword(s):  
Glycine Max ◽  
Ssr Markers ◽  
Genetic Contribution

scholarly journals Investigation of Genetic Diversity in Afghan Bread Wheat Genotypes Using SSR and AFLP Markers

2019 ◽  
Vol 7 (9) ◽  
pp. 1263
Author(s):  
Mohammad Bahman Sadeqi ◽  
Said Dadshani ◽  
Mohammad Yousefi ◽  
Gul Mohammad Ajir
Keyword(s):  
Genetic Diversity ◽  
Bread Wheat ◽  
Ssr Markers ◽  
Average Distance ◽  
Wheat Breeding ◽  
Aflp Markers ◽  
Ammonium Bromide ◽  
Aflp Data ◽  
Wheat Genotypes ◽  
Diversity Assessment

Genetic diversity assessment is the principle component for conservation and characterization of germplasm. Genetic diversity study of Afghan bread wheat genotypes is a first step to identify and to select high performance genotypes and distribute to wheat breeding programs. The main objective of this study is to investigate of genetic diversity in 35 Afghan bread wheat genotypes by using Simple Sequence Repeat (SSR) and Amplified Fragment Length Polymorphism (AFLP) markers. DNA extraction according to Cetyl Trimethyl Ammonium Bromide (CTAB) method was conducted and the total genomic DNA was isolated from each variety. Sixty-four SSR primer markers were used and eighteen EcoRI+(N)/MseI+(N) primer combinations with their primer sequences were used for selective polymerase chain reaction (PCR) amplification. Every SSR and AFLP fragment was scored as present (1) or absent (0) within all genotypes under study. Marker/ Value ratio of pairwise genetic distance between genotypes according to the SSRs data was from 0.508 to 0.691 with an average distance of 0.599. Relatively different grouping pattern in comparison to AFLP data observed through cluster analysis. Both types of molecular markers (AFLP and SSR) used in this research proved to be suitable for investigating genetic diversity in the genotypes of Afghan bread wheat, however, AFLP markers gave better view of genetically relationships among genotypes than the SSR markers. The grouping generated by AFLP data showed a special agreement with the origin regions of genotypes (Ariana-07 and Mazar-99 originating from the north of Afghanistan, Lalmi-03 and Kabul-02. Large number of DNA bands identified with AFLP markers might provide a better estimation of genetic similarity than those of SSR markers.

scholarly journals Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Min Zhou ◽  
Mei Du ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Breeding Population ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Development Of Resistance ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background: Lesion mimics (LM) is a disease-like symptom randomly spread on plant green leaves without any pathogens. Previous study has found that LM was related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can cause significant reduction of the quality and yield of crops. Development of resistance cultivars is economically and environmentally method for enhancing adaptability and yield stability of crops instead by fungicides application. In this study, we identify a novel LM gene with Pst resistance in wheat breeding population and mapped it with developing molecular markers for marker assisted selection (MAS) in wheat breeding.Results: In this study, a novel LM gene named as lm4 was identified by using a Yanzhan1/Neixiang188 RILs population, which closely linked to SSR markers Xgwm210 and Xgwm455 by 8.06 CM intervals. Then the genetic distance of the lm4 was shortened that flanked by SSR markers with 0.51CM and 0.77CM interval. Two SSR markers lm4_01_cib and lm4_02_cib were developed based on Chinese Spring genome database and wheat-660K SNP result, which can be used to conduct MAS of LM in wheat. And the results also show that lm4 significantly improved the resistance of stripe rust in wheat.Conclusions: Therefore, lm4 is associated with stripe rust resistance, and it may provide a new tool or theoretical support for future crop disease-resistant breeding and for understanding the plant apoptosis mechanism.

scholarly journals Genetic Contribution of Synthetic Hexaploid Wheat to CIMMYT’s Spring Bread Wheat Breeding Germplasm

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Umesh Rosyara ◽  
Masahiro Kishii ◽  
Thomas Payne ◽  
Carolina Paola Sansaloni ◽  
Ravi Prakash Singh ◽  
...  
Keyword(s):  
Bread Wheat ◽  
Hexaploid Wheat ◽  
Wheat Breeding ◽  
Synthetic Hexaploid Wheat ◽  
Genetic Contribution ◽  
Spring Bread Wheat ◽  
Synthetic Hexaploid ◽  
Breeding Germplasm

scholarly journals Impact of Stripe Rust Resistance Alleles on Wheat Grain Yield Using Landraces and Improved Accessions

2021 ◽  
Vol 6 (4) ◽  
Author(s):  
ElBasyoni IS ◽  
Keyword(s):  
Grain Yield ◽  
Ssr Markers ◽  
Stripe Rust ◽  
Resistance Genes ◽  
Rust Resistance ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Substantial Part ◽  
Plant Materials ◽  
Biotic Stresses

Stripe rust is one of the most devastating biotic stresses to cause grain yield losses in wheat. In the current study, 227 imported accessions, and six widely grown modern cultivars (Sids14, Sids12, Misr1, Misr2, Giza171, and Gimmiza9), were used. All plant materials were planted in the field and evaluated for stripe rust resistance and grain yield. Five Simple Sequence Repeats (SSR) markers Xpsp3000, Xbarc8, Xgwm419, Xwmc44, and Xbarc32, respectively, are associated with five essential stripe rust resistance genes Yr10, Yr15, Yr26, Yr29, and Yr59, were also used. The results indicated a highly positive and significant correlation between grain yield and stripe rust resistance. Furthermore, as the number of stripe rust resistance alleles increased, both grain yield and stripe rust resistance increased. Out of the 233 accessions used, 11 accessions were found to contain the five resistance genes. The identified resistant accessions could be used as a gene source to enhance stripe rust resistance in wheat breeding programs. SSR markers used in the current study effectively capture a substantial part of the phenotypic variation caused by stripe rust. Thus, these five markers could be used effectively in marker-assisted selection for stripe rust resistance.

scholarly journals Molecular mapping of a novel lesion mimic gene (lm4) associated with enhanced resistance to stripe rust in bread wheat

2020 ◽  
Author(s):  
Rong Liu ◽  
Jing Lu ◽  
Shigang Zheng ◽  
Min Zhou ◽  
Mei Du ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Stripe Rust ◽  
Molecular Mapping ◽  
Wheat Breeding ◽  
Stripe Rust Resistance ◽  
Genome Database ◽  
Development Of Resistance ◽  
Theoretical Support ◽  
Enhanced Resistance ◽  
Crop Disease

Abstract Background Lesion mimics (LM) is a disease-like symptom randomly spread on plant green leaves without any pathogens. Previous study has found that LM was related to enhanced resistance to a broad spectrum of diverse pathogen races and programmed cell death (PCD). Stripe rust is a globally epidemic fungal disease that can cause significant reduction of the quality and yield of crops. The development of resistance cultivars is economically and environmentally method for enhancing adaptability and yield stability of crops instead of fungicides application. In this study, a novel LM gene with Pst resistance was identified and mapped with developing molecular markers for marker assisted selection (MAS) in wheat breeding. Results In this study, a novel LM gene named as lm4 was identified by using a Yanzhan1/Neixiang188 RILs population, which closely linked to SSR markers Xgwm210 and Xgwm455 by 8.06 cM intervals. Then the genetic distance of the lm4 was shortened that flanked by SSR markers with 0.51cM and 0.77cM interval. Two SSR markers lm4_01_cib and lm4_02_cib were developed based on Chinese Spring genome database and wheat-660K SNP result, which can be used to conduct MAS of LM in wheat. And the results also show that lm4 significantly improved the resistance of stripe rust in wheat. Conclusions Therefore, lm4 is associated with stripe rust resistance which may provide a theoretical support for future crop disease-resistant breeding and for understanding the plant apoptosis mechanism.

scholarly journals Association analysis between Lipoxygenase activity and SSR markers in wheat grains

2021 ◽  
Author(s):  
W. Y. Zheng ◽  
L. Zhao ◽  
Y. M. Li ◽  
J. Li ◽  
Z. H. Zhu ◽  
...  
Keyword(s):  
Molecular Marker ◽  
Ssr Markers ◽  
Association Analysis ◽  
Marker Locus ◽  
Wheat Breeding ◽  
Wheat Cultivars ◽  
Storage Quality ◽  
Marker Loci ◽  
Processing And Storage ◽  
And Storage

AbstractLipoxygenase (LOX) activity is closely related to wheat processing and storage quality. In the present research, ten wheat cultivars were used to compare the effects of genotype, location, year, and their interactions on the LOX activity. Furthermore, 123 wheat cultivars were evaluated for LOX activity with 192 simple sequence repeat (SSR) markers and to identify elite alleles related to LOX activity. The results indicated that LOX activity was highly affected by genotype (variety) than that by the location. A total of 22 SSR molecular marker loci with a significant or very significant correlation with LOX activity were identified on performing association analysis. In 3 years, only one molecular marker locus associated with LOX activity was detected (WMC488); in 2 years, seven molecular marker loci were detected, while in only 1 year, the other 14 molecular marker loci were detected. A total of 7 and 6 marker loci significantly related to LOX activity accounting for 31.2% and 27.2%, respectively, were located in homologous groups 4 and 5, and group 7. This research provided the theoretical basis and the markers for molecular-assisted wheat breeding that facilitate the breeding process in the processing and storage quality of grains.

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