Structure Elucidation of Product of β-1,3-Glucosyltransferase Encoded by wfgD Gene in Escherichia Coli O152 Using Mass Spectrometry

2010 ◽  
Vol 38 (2) ◽  
pp. 225-228
Author(s):  
Da-Wei ZHOU ◽  
Bo HU ◽  
Bin LIU ◽  
Jun-Li WU ◽  
Yan-Fang HAN
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Structure Elucidation ◽  
I Gene

Isolation of Epigallocatechin Gallate from Green Tea and its Effects on Probiotics and Pathogenic Bacteria

2017 ◽  
Vol 17 (1) ◽  
pp. 69-77
Author(s):  
Tu Lijun ◽  
Sun Hanju ◽  
He Shudong ◽  
Zhu Yongsheng ◽  
Yu Ming ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Green Tea ◽  
Lactobacillus Acidophilus ◽  
Pathogenic Bacteria ◽  
Epigallocatechin Gallate ◽  
Microbial Populations ◽  
Medium Ph ◽  
Bioactive Substance ◽  
Tea Extract

The aim of this study was to investigate epigallocatechin gallate (EGCG) prebiotics activities systematically which was reported as a bioactive substance. Therefore, EGCG was separated by water extraction, resin purification and prep-HPLC. Then the production of EGCG was confirmed by HPLC and mass spectrometry (MS) analysis and its purify was 97.23%. EGCG extractive and green tea extract (GTE) were further incubated with Bifidobacterium infantis, B. adolescentis, B. bifidum and Lactobacillus acidophilus to study its effect on microbial populations and medium pH. Finally, Escherichia coli, Salmonella, Staphylococcus aureus and Candida albicans were employed as pathogenic bacteria to explore the antimicrobial activity of EGCG and GTE. The results demonstrated that EGCG extractive could be beneficial for the proliferation of Bifidobacterium and L. acidophilus and also inhibit some pathogenic bacteria. In conclusion, both EGCG extractive and GTE had prebiotics activities and the effects of EGCG extractive were superior to those of GTE.

Alkylation of Escherichia coli Thioredoxin by S-(2-Chloroethyl)glutathione and Identification of the Adduct on the Active Site Cysteine-32 by Mass Spectrometry

1995 ◽  
Vol 8 (7) ◽  
pp. 934-941 ◽  
Author(s):  
John C. L. Erve ◽  
Elisabeth Barofsky ◽  
Douglas F. Barofsky ◽  
Max L. Deinzer ◽  
Donald J. Reed
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Active Site ◽  
Active Site Cysteine

Mass Spectrometry of Escherichia coli RNA PolymeraseInteractions of the Core Enzyme with σ70 and Rsd Protein

Structure ◽  
2004 ◽  
Vol 12 (2) ◽  
pp. 269-275 ◽  
Author(s):  
L ILAG ◽  
L WESTBLADE ◽  
C DESHAYES ◽  
A KOLB ◽  
S BUSBY ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
The Core ◽  
Core Enzyme

scholarly journals A rapid diagnostic workflow for cefotaxime-resistant Escherichia coli and Klebsiella pneumoniae detection from blood cultures by MALDI-TOF mass spectrometry

PLoS ONE ◽  
2017 ◽  
Vol 12 (10) ◽  
pp. e0185935 ◽  
Author(s):  
Elena De Carolis ◽  
Silvia Paoletti ◽  
Domenico Nagel ◽  
Antonietta Vella ◽  
Enrica Mello ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Klebsiella Pneumoniae ◽  
Blood Cultures ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals Metabolic profiling of Escherichia coli by ion mobility-mass spectrometry with MALDI ion source

2010 ◽  
Vol 45 (12) ◽  
pp. 1383-1393 ◽  
Author(s):  
Prabha Dwivedi ◽  
Geoffery Puzon ◽  
Maggie Tam ◽  
Denis Langlais ◽  
Shelley Jackson ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Ion Mobility ◽  
Metabolic Profiling ◽  
Ion Source ◽  
Ion Mobility Mass Spectrometry
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