Primary study on computer-aided qualitative and quantitative radial thin-layer chromatography methods for analysis of Yiqing tablets

2019 ◽  
Vol 37 (11) ◽  
pp. 1209
Author(s):  
Chunjie TIAN ◽  
Mingxin CHEN ◽  
Danqi WANG ◽  
Hongling WANG ◽  
Jianhuan GUAN ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Primary Study ◽  
Qualitative And Quantitative ◽  
Computer Aided ◽  
Layer Chromatography

Urinary Homogentisic Acid: Determination by Thin-Layer Chromatography

1973 ◽  
Vol 19 (5) ◽  
pp. 459-462 ◽  
Author(s):  
Jerome M Feldman ◽  
June Bowman
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clinical Laboratory ◽  
Homogentisic Acid ◽  
Qualitative And Quantitative ◽  
Color Development ◽  
Layer Chromatography ◽  
Phenol Reagent ◽  
Acid Determination

Abstract A new method is described for qualitative and quantitative determination of urinary homogentisic acid. The method involves extraction of urine with ethyl acetate, thin-layer chromatography of the extract on Silica Gel G, elution of the homogentisic acid into water, and color development with Folin’s phenol reagent. Absorbance is maximum at 750 nm and linear to a concentration of at least 5 mg of homogentisic acid per milliliter of urine. The method is highly specific for homogentisic acid; added gentisic acid, 3,4-dihydroxyphenylacetic acid, ascorbic acid, or L-3,4 dihydroxyphenylalanine do not interfere. The coefficient of variation "in-run" is 5.3%, "between-run" 6.8%. Using this method we have demonstrated a marked variation in day-to-day homogentisic acid excretion in a patient with alcaptonuria. This method, which offers some advantages over existing techniques, should be suitable for use in a clinical laboratory.

Computer-aided optimization of stepwise gradient profiles in thin-layer chromatography

1992 ◽  
Vol 34 (5-8) ◽  
pp. 303-307 ◽  
Author(s):  
G. Matysik ◽  
W. Markowski ◽  
E. Soczewiński ◽  
B. Polak
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Computer Aided ◽  
Stepwise Gradient ◽  
Layer Chromatography
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