Aim: The present work was aimed at evolving pharmacognostical and phytochemical quality standards for certain traditional herbs like Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum. These three plants are reported to possess antidiabetic activity.
Study Design: The plants were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis, phytochemical properties, and thin layer chromatography (TLC)
Place and Duration of Study: The study was carried out at College of Pharmacy, Dr. APJ Abdul Kalam University, Indore, during 2019-20
Methodology: The material were collected, authenticated and Macro-morphological, qualitative and quantitative microscopic features as well as physicochemical, fluorescence analysis, phytochemical properties, and thin layer chromatography (TLC) profile of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum were determined using standard methods.
Results: The macroscopical and microscopical studies revealed useful diagnostic features. Phytochemical screening reveals the presence of secondary metabolites, physicochemical including fluorescence analysis of powdered drug proved useful to differentiate the powdered drug material. Thin layer chromatography analysis showed the presence of important phytoconstituents such Phyllanthin, hypophyllanthin, glycerrizin and piperine
Conclusion: The data generated from this study would serve as useful gauge for determining the quality of Phyllanthus amarus, Glycerrhiza glabra and Piper nigrum thereby correct identification and authentification of these plants.
It would also help scientists to utilize such needful information regarding the plants identity and characteristics in building new polyherbal formulations.
Simultaneous qualitative and quantitative analysis of Tangkening granule using thin layer chromatography (TLC) and high performance liquid chromatography (HPLC)
