Paternity analysis and pollen dispersal for the second generation clonal seed orchard of Pinus massoniana

2011 ◽  
Vol 35 (9) ◽  
pp. 937-945 ◽  
Author(s):  
Xiao-Mei TAN ◽  
Zhi-Chun ZHOU ◽  
Guo-Qing JIN ◽  
Yi ZHANG
Keyword(s):  
Second Generation ◽  
Pollen Dispersal ◽  
Seed Orchard ◽  
Pinus Massoniana ◽  
Paternity Analysis ◽  
Clonal Seed Orchard

Paternity analysis with microsatellites in a Danish Abies nordmanniana clonal seed orchard reveals dysfunctions

2006 ◽  
Vol 36 (4) ◽  
pp. 1054-1058 ◽  
Author(s):  
O K Hansen ◽  
E D Kjær
Keyword(s):  
Asymptotic Variance ◽  
Seed Orchard ◽  
Paternity Analysis ◽  
Effective Population ◽  
Population Number ◽  
Clonal Seed Orchard ◽  
Relative Contribution ◽  
Male Gametes ◽  
Abies Nordmanniana ◽  
The Status

A paternity analysis using five microsatellite markers was conducted in a Danish clonal seed orchard with 13 Abies nordmanniana (Stev.) Spach clones. The purpose was to investigate potential seed-orchard dysfunctions, with special emphasis on nonequal pollen contributions and selfing. Male paternity was found for 232 seedlings germinated from seeds collected on three ramets, each of eight clones, and the relative contribution of each clone to the gene pool of male gametes was calculated. Furthermore, 49 ramets were genotyped to check for erroneous grafting. The effect of an unbalanced male contribution was quantified by means of two measures: (1) the status number (NS), which reflects buildup of coancestry in the seed-orchard crop as a result of a low number of clones and an unequal male contribution, and (2) the asymptotic variance effective population number (Ne(v)). The contributions by pollen donors from the 13 clones were highly skewed. Three clones were fathers to more than 75% of the progenies, while making up only 24% of the ramets in the seed orchard. Four clones sired no progenies at all. The unequal contribution on the male side corresponded to NS = 4.2 and Ne(v) = 5.8. Some selfing was observed, which may give rise to concern if clonal seed orchards with few clones are established. The estimated maximum pollen contamination from outside the seed orchard was 4.3%. No grafting–labelling errors were identified.

scholarly journals Early Realized Gains for Two-cycle Selection for Black Spruce and Their Implications for Testing Effort Allocation

2011 ◽  
Vol 60 (1-6) ◽  
pp. 178-186 ◽  
Author(s):  
Y. H. Weng
Keyword(s):  
First Generation ◽  
Second Generation ◽  
Black Spruce ◽  
Seed Orchard ◽  
Significance Level ◽  
Clonal Seed Orchard ◽  
Seedling Seed Orchard ◽  
Silvicultural Practice ◽  
Sib Families ◽  
Realized Genetic Gains

AbstractDeployment of improved black spruce (Picea mariana(Mill.) B.S.P.) seedlots in New Brunswick (NB) is a standard silvicultural practice. Most plantations have been established using elite-stand seedlots in the 1980s, first-generation seedling seed orchard seedlots in the 1990s and thereafter second-generation clonal seed orchard seedlots. A large-plot realized gain test was established at six sites in NB to provide estimates of actual gains from planting these improved seedlots. The test compared four improved seedlots, representing seedlots collecting from an elite stand, a first-generation seed orchard, two second-generation orchards and a mix of eight elite full-sib families identified in second-generation testing populations, with one unimproved checklot. This paper presents height measurements taken on trees at age 5. Results indicate that realized genetic gains from two-cycle selection and breeding are reasonably high and, in general, the more improved the seedlot, the higher the gain. Planting the elite-stand or the first-generation orchard seedlot would produce a gain of about 10% (relative to the checklot) in 5-yr height and an additional 5.8% gain could be obtained from planting the second-generation orchard seedlot. Even higher gain could be obtained via the deployment of the elite full-sib families, which was estimated to be about 8.6% more than the deployment of the second-generation orchard seedlot. While the realized gain varied with site, the ranking of gain achieved for the different seedlots was similar between sites. The above information was further used to optimize testing efforts for realized gain tests. To detect a typical gain of 5 to 10% at a significance level of 0.05 with a predetermined power of 0.80, each test should include 5 to 10 blocks per site (the number of sites is fixed at 4) or 4 to 6 sites (the number of blocks per site is fixed at 6) paired with planting 36 to 49 trees per plot. These approximate numbers of sites and blocks per site should be modified, depending on the interactions of seedlot with site and with block within site.

scholarly journals Model of experimental clonal seed orchard for the production of Serbian spruce sPicea omorika /Panc./Purkyne) intraspecific hybrids

Genetika ◽  
2002 ◽  
Vol 34 (1) ◽  
pp. 11-19
Author(s):  
Mirjana Sijacic-Nikolic ◽  
Vasilije Isajev
Keyword(s):  
Second Generation ◽  
Diallel Cross ◽  
Seed Orchard ◽  
Clonal Seed Orchard ◽  
Seedling Seed Orchard ◽  
Applied Study ◽  
Genetic Potential ◽  
Incomplete Diallel Cross ◽  
Serbian Spruce ◽  
Intraspecific Hybrids

The presented model for the establishment of an experimental clonal seed orchard of Serbian spruce was designed based on the results of the analysis and assessment of the genetic potential of Serbian spruce seedling seed orchard at Godovik. Based on the results of the analyses, eight superior half-sib lines of Serbian spruce were selected, of which 24 genotypes were selected. Their hybridisation, by the model of incomplete diallel cross resulted in 21 combinations at the level of half-sib lines, i.e. 48 combinations at the level of parent genotypes. The applied study methods identified the potentially valuable genotypes-cone producers i.e. pollinators, which will be fixed by cloning in the seed orchard of the second generation for the production of the promising hybrids.

Inter-year variation in selfing, background pollination, and paternal contribution in a Norway spruce clonal seed orchard

2014 ◽  
Vol 44 (7) ◽  
pp. 760-767 ◽  
Author(s):  
Monika Dering ◽  
Andrzej Misiorny ◽  
Władysław Chałupka
Keyword(s):  
Picea Abies ◽  
Microsatellite Markers ◽  
Norway Spruce ◽  
Pollen Dispersal ◽  
Geographic Origin ◽  
Seed Orchard ◽  
Seed Crop ◽  
Clonal Seed Orchard ◽  
Paternal Contribution ◽  
Nuclear Microsatellite

We aimed to investigate inter-year variability in mating system and paternity in an experimental Norway spruce (Picea abies (L.) Karst.) clonal seed orchard that was established with five geographically distinct populations. Using five nuclear microsatellite markers, we analysed 1396 progeny of five mother trees representing each of the populations in three successive mast-years. Selfing varied among mother trees, ranging from 0% to 18.3% with a mean of 13.9% in 1994, 10.1% in 2004, and 7.6% in 2006. Considerable background pollination was observed in each of the three studied mast-years and reached ca. 58%. The paternity assignment conducted showed an uneven male contribution to the seed crop, what was partly related to the number of ramets per paternal clone. All provenances contributed to the seed crop, but with different input, and two provenances dominated. Effective pollen dispersal was significantly determined by distance, with 72.2% of the seed crop resulting from crosses at a distance of up to 15 m. Our study indicated that despite considerable differences in the geographic origin of populations, inter-mating between clones from each population was possible, which generally agrees with the expectations related to the establishment of this seed orchard.

scholarly journals Estimation of Genetic and Phenotypic Parameters for Growth Traits in a Clonal Seed Orchard of Pinus kesiya in Malawi

ISRN Forestry ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Edward Missanjo ◽  
Gift Kamanga-Thole ◽  
Vidah Manda
Keyword(s):  
Growth Traits ◽  
Seed Orchard ◽  
Direct Selection ◽  
Tree Model ◽  
Individual Tree ◽  
Individual Level ◽  
Clonal Seed Orchard ◽  
Pinus Kesiya ◽  
Individual Tree Model ◽  
The Individual

Genetic and phenotypic parameters for height, diameter at breast height (dbh), and volume were estimated for Pinus kesiya Royle ex Gordon clonal seed orchard in Malawi using an ASReml program, fitting an individual tree model. The data were from 88 clones assessed at 18, 23, 30, 35, and 40 years of age. Heritability estimates for height, dbh, and volume were moderate to high ranging from 0.19 to 0.54, from 0.14 to 0.53, and from 0.20 to 0.59, respectively, suggesting a strong genetic control of the traits at the individual level, among families, and within families. The genetic and phenotypic correlations between the growth traits were significantly high and ranged from 0.69 to 0.97 and from 0.60 to 0.95, respectively. This suggests the possibility of indirect selection in trait with direct selection in another trait. The predicted genetic gains showed that the optimal rotational age of the Pinus kesiya clonal seed orchard is 30 years; therefore, it is recommended to establish a new Pinus kesiya clonal seed orchard. However, selective harvest of clones with high breeding values in the old seed orchard should be considered so that the best parents in the old orchard can continue to contribute until the new orchard is well established.

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