Cloning and Expression of a Gene Encoding a Raffinose Synthase in the Resurrection Plant Boea hygrometrica

2012 ◽  
Vol 47 (1) ◽  
pp. 44-54 ◽  
Author(s):  
Wang Zhi ◽  
Liu Yongxiu ◽  
Wei Jianhua ◽  
Deng Xin
Keyword(s):  
Cloning And Expression ◽  
Resurrection Plant ◽  
Gene Encoding ◽  
Raffinose Synthase ◽  
Boea Hygrometrica

Molecular cloning and differential expression of sHSP gene family members from the resurrection plant Boea hygrometrica in response to abiotic stresses

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Zhennan Zhang ◽  
Bo Wang ◽  
Dongmei Sun ◽  
Xin Deng
Keyword(s):  
Molecular Chaperones ◽  
Abiotic Stresses ◽  
Potential Role ◽  
Resurrection Plant ◽  
High Calcium ◽  
Shsp Gene ◽  
Alkaline Conditions ◽  
Boea Hygrometrica ◽  
Shock Proteins

AbstractSmall heat shock proteins (sHSPs) are a class of molecular chaperones that bind to and prevent aggregation of proteins. To assess the potential role of sHSPs in protection against abiotic stresses, we conducted a screening of sHSP genes from the desiccation-tolerant resurrection plant Boea hygrometrica, which is widespread in East Asia in alkaline, calcium-rich limestone crevices. In total, 25 sHSP genes, belonging to six subgroups, were identified from the desiccated leaves of B. hygrometrica. Ten of these genes were cloned and named according to the nomenclature proposed for sHSPs. Transcripts of all these BhsHSPs were detectable in fresh leaves, but only 6 genes were induced after desiccation, and remained high during rehydration. Four of the cytosol-targeted BhsHSP genes were up-regulated under treatments, such as heat, cold, alkaline conditions, high calcium, oxidation, or application of the phytohormone abscisic acid. Together, these results demonstrate that CI and CII sHSPs, especially Bh17.9CI and Bh17.4BCII, are associated with abiotic stresses, and may function in the maintenance of protein stability, aiding in the adaptations to extreme environmental conditions in which B. hygrometrica can survive.

Cloning and Expression of the xynA1 Gene Encoding a Xylanase of the GH10 Group in Caulobacter crescentus

2015 ◽  
Vol 175 (8) ◽  
pp. 3915-3929 ◽  
Author(s):  
Luciana Graciano ◽  
Juliana Moço Corrêa ◽  
Fabíola Giovanna Nesello Vieira ◽  
Adilson Bosetto ◽  
Eduardo Alexandre Loth ◽  
...  
Keyword(s):  
Caulobacter Crescentus ◽  
Cloning And Expression ◽  
Gene Encoding
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