scholarly journals Effects of Garlic Rotation and Organic Fertilizer Application on Bacterial Community Structure in Rhizosphere Soil of Continuous Cropping Tomato

2020 ◽  
pp. 25-36
Author(s):  
Yanyan Zhao ◽  
Guangjing Yin ◽  
Zuomin Song ◽  
Baoye Chen ◽  
Qiangfeng Li
Keyword(s):  
Alkaline Phosphatase ◽  
Community Structure ◽  
Bacterial Community ◽  
Rhizosphere Soil ◽  
Early Stage ◽  
Organic Fertilizer ◽  
Fertilizer Application ◽  
Control Treatment ◽  
Continuous Cropping ◽  
Tomato Plants

16S/18S/ITS's amplicon and metagenome sequencing had been used extensively to investigate the root mi- crobial community structure of crops and model plants. In this study, the rhizosphere soils of the continuous cropping (Control), garlic rotation and organic fertilizer application tomato plants were taken as the test samples, respectively, to measure the microbial community structure and soil enzyme activities. The results showed that both planting modes had significant effects on the microecology of continuous cropping tomato rhizosphere soil. After garlic rotation, the diversity (Shannon index and Simpson index) and relative abundance (Ace index and Chao1 index) of soil bacterial community decreased significantly in the early stage, but increased significantly in the later stage. These changes could be significantly alleviated by the application of organic fertilizer. During the whole growth period of tomato plants, the activities the six soil enzymes (sucrase, catalase, urease, protease, amylase and alkaline phosphatase) were all the highest in the organic fertilizer application plot, followed by the rotation plot and the continuous cropping plot. With  the extension of planting time, the activities of soil protease, urease and alkaline phosphatase increased gradually, while that of sucrase decreased gradually. Catalase activity initially increased and then decreased, while amylase activity did not change significantly with time. After garlic rotation, mortality rate of tomato plants decreased significantly, and all growth indexes were significantly better than those of the control treatment. To sum up, garlic/tomato rotation can effectively improve the soil environment and alleviate the continuous cropping obstacle of tomatoes in greenhouse. Moreover, rotation combining with organic fertilizer application can acquire the most obvious mitigation effect.

scholarly journals Effects of Manure and Chemical Fertilizer on Bacterial Community Structure and Soil Enzyme Activities in North China

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1017
Author(s):  
Zhiping Liu ◽  
Wenyan Xie ◽  
Zhenxing Yang ◽  
Xuefang Huang ◽  
Huaiping Zhou
Keyword(s):  
Alkaline Phosphatase ◽  
Community Structure ◽  
Bacterial Community ◽  
Enzyme Activities ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Organic Fertilizer ◽  
Chemical Fertilizer ◽  
Soil Bacterial Communities ◽  
Soil Bacterial

The application of organic fertilizer affects soil microbes and enzyme activities. In this study, we explored the effects of various long-term different fertilization treatments (manure, M; chemical fertilizer, NP; manure + chemical fertilizer, MNP; and no fertilizer, CK) on bacterial community structure and soil sucrase, urease, and alkaline phosphatase activities in Shaping, Hequ, China. High-throughput sequencing was used to amplify the third to the fourth hypervariable region of the 16S ribosomal RNA for analysis of the bacterial community structure. Enzyme activities were determined by colorimetry. Soil treated with MNP had the highest bacterial Abundance-based Coverage Estimator index and enzyme activities. The principal coordinates analysis results showed significant differences among the various fertilization treatments (p < 0.001). Proteobacteria, Actinobacteria, Acidobacteria, Gemmatimonadetes, and Chloroflexi were consistently dominant in all soil samples. The redundancy analysis and Monte Carlo permutation tests showed that the soil bacterial communities were significantly correlated with alkali-hydrolyzable nitrogen, organic matter, urease, and alkaline phosphatase. Our results reveal the fundamentally different effects that organic and inorganic fertilizers have on soil bacterial communities and their functions.

scholarly journals Earthworm activity optimized the rhizosphere bacterial community structure and further alleviated the yield loss in continuous cropping lily (Lilium lancifolium Thunb.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yaoxiong Lu ◽  
Peng Gao ◽  
Yunsheng Wang ◽  
Wei Li ◽  
Xinwei Cui ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Bacterial Community ◽  
Soil Microbial Community ◽  
Bacterial Community Structure ◽  
Rhizosphere Soil ◽  
The Other ◽  
Continuous Cropping ◽  
Soil Microbial ◽  
Rhizosphere Bacterial Community

AbstractThe soil microbial community plays a vital role in the biogeochemical cycles of bioelements and maintaining healthy soil conditions in agricultural ecosystems. However, how the soil microbial community responds to mitigation measures for continuous cropping obstacles remains largely unknown. Here we examined the impact of quicklime (QL), chemical fungicide (CF), inoculation with earthworm (IE), and a biocontrol agent (BA) on the soil microbial community structure, and the effects toward alleviating crop yield decline in lily. High-throughput sequencing of the 16S rRNA gene from the lily rhizosphere after 3 years of continuous cropping was performed using the Illumina MiSeq platform. The results showed that Proteobacteria, Acidobacteria, Bacteroidetes, Actinobacteria, Chloroflexi and Gemmatimonadetes were the dominant bacterial phyla, with a total relative abundance of 86.15–91.59%. On the other hand, Betaproteobacteriales, Rhizobiales, Myxococcales, Gemmatimonadales, Xanthomonadales, and Micropepsales were the dominant orders with a relative abundance of 28.23–37.89%. The hydrogen ion concentration (pH) and available phosphorus (AP) were the key factors affecting the structure and diversity of the bacterial community. The yield of continuous cropping lily with using similar treatments decreased yearly for the leaf blight, but that of IE was significantly (p < 0.05) higher than with the other treatments in the same year, which were 17.9%, 18.54%, and 15.69% higher than that of blank control (CK) over 3 years. In addition, IE significantly (p < 0.05) increased organic matter (OM), available nitrogen (AN), AP, and available potassium (AK) content in the lily rhizosphere soil, optimized the structure and diversity of the rhizosphere bacterial community, and increased the abundance of several beneficial bacterial taxa, including Rhizobiales, Myxococcales, Streptomycetales and Pseudomonadales. Therefore, enriching the number of earthworms in fields could effectively optimize the bacterial community structure of the lily rhizosphere soil, promote the circulation and release in soil nutrients and consequently alleviate the loss of continuous cropping lily yield.

scholarly journals Effects of Continuous Cropping on Bacterial Community Structure in Rhizospheric Soil of Sweet Potato 

2020 ◽  
Author(s):  
Zhiyuan Gao ◽  
Yaya Hu ◽  
Meikun Han ◽  
Junjie Xu ◽  
Xue Wang ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Sweet Potato ◽  
Soil Ph ◽  
Bacterial Community Structure ◽  
Continuous Cropping ◽  
Rhizospheric Soil ◽  
Sweet Potatoes ◽  
Potato Varieties ◽  
Soil Bacterial Community Structure

Abstract Background: Continuous cropping obstacles from sweet potato are widespread, which seriously reduce the yield and quality, cause certain economic losses. Bacteria of Rhizospheric soil are the richest and are associated with obstacles to continuous cropping. However, few studies on how continuous sweet potato cropping affects the rhizospheric soil bacterial community structure. In the study, Illumina Miseq method was used to explore rhizosphere soil bacterial community structure changes with different sweet potato varieties, and the correlation between soil characteristics and this bacterial community after continuous cropping, to provide theoretical guidance for prevention and treatment of sweet potatoes continuous cropping obstacles. Results: After continuous cropping two years, the results showed that (1) the dominant bacterial phlya in rhizospheric soils from both Xushu18 and Yizi138 were Proteobacteria, Acidobacteria, and Actinobacteria. The most dominant genus was Subgroup 6_norank. The relative abundance of rhizospheric soil bacteria of two sweet potato varieties changed significantly. (2) The richness and diversity indexes of bacteria in Xushu18 rhizospheric soil were higher than those from Yizi138 after continuous cropping. Moreover, the beneficial Lysobacter and Bacillus were more prevalent in Xushu18, but Yizi138 contained more harmful Gemmatimonadetes. (3) Soil pH decreased after continuous cropping, and redundancy analysis result indicated that soil pH was correlated significantly with bacterial community. Spearman’s rank correlations coefficients analysis demonstrated that pH was positively associated with Planctomycetes and Acidobacteria, but negatively associated with Actinobacteria and Firmicutes.Conclusions: After continuous cropping, the bacterial community structure and physicochemical properties of sweet potato rhizospheric soil were unbalanced, and the changes from different sweet potato varieties were different. The contents of Lysobacter and Bacillus were higher in the sweet potato variety resistant to continuous cropping. It provides a basis for developing new microbial fertilizer for sweet potatoes to alleviate continuous cropping obstacle.

scholarly journals Effects of Continuous Cropping on Bacterial Community Structure in Rhizospheric Soil of Sweet Potato

2020 ◽  
Author(s):  
Zhiyuan Gao ◽  
Yaya Hu ◽  
Meikun Han ◽  
Junjie Xu ◽  
Xue Wang ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Sweet Potato ◽  
Soil Ph ◽  
Bacterial Community Structure ◽  
High Throughput Sequencing ◽  
Potato Industry ◽  
Continuous Cropping ◽  
Rhizospheric Soil ◽  
Potato Varieties

Abstract Background: Continuous cropping obstacles from sweet potato are widespread, which seriously reduce the yield and quality, restrict the sustainable development of sweet potato industry. Bacteria are the most abundant in rhizospheric soil and have a certain relationship with continuous cropping obstacles. However, there are few reports on how continuous cropping affected the bacterial community structure in the rhizospheric soil of sweet potato. In this study, high-throughput sequencing technique was used to explore the changes of rhizospheric soil bacterial community structure of different sweet potato varieties, and the correlation between soil characteristics and this bacterial community after continuous cropping, so as to provide a theoretical basis for the prevention and control of sweet potato continuous cropping obstacles.Results: After two years of continuous cropping, the results showed that (1) the dominant bacteria phlya in rhizospheric soils from both Xushu18 and Yizi138 were Proteobacteria, Acidobacteria, and Actinobacteria. The most dominant genus was Subgroup 6_norank. Significant changes in the relative abundance of rhizospheric soil bacteria were observed for two sweet potato varieties. (2) Bacterial richness and diversity indexes of rhizospheric soil from Xushu18 were higher than those from Yizi138 after continuous cropping. Moreover, the beneficial Lysobacter and Bacillus were more prevalent in Xushu18, but Yizi138 contained more harmful Gemmatimonadetes. (3) Soil pH decreased after continuous cropping, and redundancy analysis showed that soil pH was significantly correlated with bacterial community. Spearman’s rank correlations coefficients analysis demonstrated that pH was positively correlated with Planctomycetes and Acidobacteria, and negatively correlated with Actinobacteria and Firmicutes.Conclusions: After continuous cropping of sweet potato, the bacterial community structure and physicochemical properties in the rhizospheric soil were unbalanced, and the changes of different sweet potato varieties were different. The contents of Lysobacter and Bacillus were higher in the sweet potato variety resistant to continuous cropping. It provides a basis for the development of special microbial fertilizer for sweet potatoes to alleviate continuous cropping obstacle.

Characterization of Bacterial Community Structure in Rhizosphere Soil of Grain Legumes

2005 ◽  
Vol 49 (3) ◽  
pp. 407-415 ◽  
Author(s):  
S. Sharma ◽  
M.K. Aneja ◽  
J. Mayer ◽  
J.C. Munch ◽  
M. Schloter
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
Rhizosphere Soil ◽  
Grain Legumes

scholarly journals Difference in Rhizosphere Soil Bacterial Community was among Six Cinnamomum camphora Chemotypes

2021 ◽  
Author(s):  
Zufei Xiao ◽  
Beihong Zhang ◽  
Yangbao Wang ◽  
Zhinong Jin ◽  
Feng Li ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Rhizosphere Soil ◽  
Soil Nutrient ◽  
Soil Bacterial Community ◽  
Cinnamomum Camphora ◽  
Close Relationship ◽  
Soil Bacterial

Abstract: Plant types and soil bacterial communities had a close relationship, understanding the profound association between them contributes to better learn bacterial ecological function for plant growth. In this study, rhizosphere soil of six different chemotype Cinnamomum camphora trees were collected, including C. bodinieri var. citralifera, [C. camphora (Linn.) Presl], camphora-type, cineole-type, linalool-type and isoborneol-type. Soil properties content and bacterial communities were analyzed. Two chemotype C. camphora, including [C. camphora (Linn.) Presl] and linalool-type, shaped similar bacterial community structure, decreased Firmcutes relative abundance. richness estimators (Chao1 index and Ace index) of [C. camphora (Linn.) Presl] were decreased compared with the others. Furthermore, soil bacterial community structure was also similar among bodinieri var. citralifera, camphora-type, cineole-type and isoborneol-type. Hence, different chemotype C. camphora altered soil nutrient and shaped rhizosphere bacterial communities.

scholarly journals Effects of Continuous Cropping of Sweet Potatoes on the Bacterial Community Structure in Rhizospheric Soil

2020 ◽  
Author(s):  
Zhiyuan Gao ◽  
Yaya Hu ◽  
Meikun Han ◽  
Junjie Xu ◽  
Xue Wang ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Sweet Potato ◽  
Soil Ph ◽  
Bacterial Community Structure ◽  
Continuous Cropping ◽  
Rhizospheric Soil ◽  
Sweet Potatoes ◽  
Potato Varieties ◽  
Soil Bacterial Community Structure

Abstract Background: Continuous cropping obstacles from sweet potatoes are widespread, which seriously reduce the yield and quality, causing certain economic losses. Bacteria of rhizospheric soil are the richest and are associated with obstacles to continuous cropping. However, few studies have examined how continuous sweet potato cropping affects the rhizospheric soil bacterial community structure. Results: In the study, the Illumina MiSeq method was used to explore the variations inrhizospheric soil bacterial community structure of different sweet potato varieties after continuous cropping, as well as the correlation between soil characteristics and the bacterial community. The results showed that (1) the dominant bacterial phyla in rhizospheric soils from both Xushu 18 and Yizi 138 were Proteobacteria, Acidobacteria, and Actinobacteria. The most dominant genus was Subgroup 6_norank. The relative abundance of rhizospheric soil bacteria varied significantly between the two sweet potato varieties. (2) The richness and diversity indexes of bacteria were higher in Xushu 18 rhizospheric soil than in Yizi 138 soil after continuous cropping. Moreover, beneficial Lysobacter and Bacillus were more prevalent in Xushu 18, while Yizi 138 contained more harmful Gemmatimonadetes. (3) Soil pH decreased after continuous cropping, and redundancy analysis indicated that soil pH was significantly correlated with the bacterial community. Spearman’s rank correlation coefficient analysis demonstrated that pH was positively associated with Planctomycetes and Acidobacteria, but negatively associated with Actinobacteria and Firmicutes.Conclusions: After continuous cropping, the bacterial community structure and physicochemical properties of sweet potato rhizospheric soil were changed, and the changes from different sweet potato varieties were different. The contents of Lysobacter and Bacillus were higher in the sweet potato variety resistant to continuous cropping. It provides a basis for developing new microbial fertilizers for sweet potatoes to alleviate the continuous cropping obstacle.

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