scholarly journals Assessment of Antibiotic Influence on Structural Modifications of Amniotic Membrane by FTIR Spectroscopy

2019 ◽  
Vol 57 (2) ◽  
pp. 191-198
Author(s):  
George Roiu ◽  
Simona Cavalu ◽  
Alin Teusdea ◽  
Denisa A. Petricas-Heredea ◽  
Ovidiu Fratila
Keyword(s):  
Extracellular Matrix ◽  
Ftir Spectroscopy ◽  
Tissue Regeneration ◽  
Amniotic Membrane ◽  
Laminar Structure ◽  
Microbial Properties ◽  
Structural Modifications ◽  
Gentamicin Treatment ◽  
Deconvolution Techniques ◽  
Secondary Structure Composition

The amniotic membrane is a readily available biomaterial with an important potential for tissue regeneration in dermatology and ophtalmology, with anti-inflammatory and anti-microbial properties. The extracellular matrix of the amniotic membrane is composed mainly of collagen, fibronectin and laminin. The purpose of our study was to investigate the structural modifications of collagen extracellular matrix of amniotic membrane upon interaction with two different antibiotics, frequently used in surgical and post- surgical procedure, respectively ciprofloxacin and gentamicin. SEM micrographs evidenced the ultrastructure features of dried amniotic membrane, with laminar structure, flexible, transparent, with no blood vesels or nerves. FTIR spectroscopy combined with deconvolution techniques was applied with the aim to determine the extent of denaturation upon treatment with different antibiotics. By spectral analysis, we concluded that gentamicin treatment is more favorable compared to ciprofloxacin, as the denaturation process is reflected by the lower sheet/turns ratio of the secondary structure composition.

scholarly journals Nano-Scale Modifications of Amniotic Membrane Induced by UV and Antibiotic Treatment: Histological, AFM and FTIR Spectroscopy Evidence

Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 863
Author(s):  
Simona Cavalu ◽  
George Roiu ◽  
Ovidiu Pop ◽  
Denisa A. Petricas Heredea ◽  
Traian Octavian Costea ◽  
...  
Keyword(s):  
Ftir Spectroscopy ◽  
Antibiotic Treatment ◽  
Amniotic Membrane ◽  
Ocular Surface ◽  
Enzymatic Digestion ◽  
Uv Exposure ◽  
Amniotic Membrane Transplantation ◽  
Corneal Tissue ◽  
Nano Scale ◽  
Gentamicin Treatment

The efficiency of amniotic membrane (AM) transplantation in different types of ocular surface disorders is due to its outstanding properties such as antifibrotic, antibacterial, anti-inflammatory and antiangiogenic, working as a versatile scaffold to promote corneal tissue epithelialization. A proper preparation, preservation and clinical application are crucial for the best outcomes in the treatment of different severe ocular disorders, taking into account its fragility. In this context, by combining high-sensitivity tools such as atomic force microscopy (AFM) and Fourier transform infrared (FTIR) spectroscopy with histological and immunohistochemical examination, we aimed to investigate the ultrastructural modifications of the amniotic membrane (AM) upon UV exposure and/or antibiotic treatment, with relevance for clinical applications in ocular surface surgery. From the morphological point of view, we noticed a loss of cuboidal cells in the basal membrane, accompanied by the splitting of collagen fibers upon UV and/or gentamicin treatment, while structural alteration of proteins was evidenced by the FTIR quantitative analysis of the secondary structure. A decrease in α-helix and β-sheet content, accompanied by increased content in less ordered structures (turns, random and side chains), was noticed after all the treatments. At the nano-scale, AFM details showed modifications of collagen fibrils in terms of their thickness and network compaction upon gentamicin and/or UV treatment. The enzymatic digestion assay demonstrated that UV exposure significantly reduces the degradation rate of the AM, while gentamicin treatment promotes an accelerated enzymatic digestion upon UV exposure. In order to highlight the clinical impact of the research, a clinical case is presented showing the relevance of amniotic membrane transplantation in pterygium surgery.

scholarly journals Extracellular matrix‐based scaffolding technologies for periodontal and peri‐implant soft tissue regeneration

2019 ◽  
Vol 91 (1) ◽  
pp. 17-25 ◽  
Author(s):  
Lorenzo Tavelli ◽  
Michael K. McGuire ◽  
Giovanni Zucchelli ◽  
Giulio Rasperini ◽  
Stephen E. Feinberg ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Soft Tissue ◽  
Tissue Regeneration ◽  
Soft Tissue Regeneration

Biomimetic Porous PLGA Scaffolds Incorporating Decellularized Extracellular Matrix for Kidney Tissue Regeneration

2016 ◽  
Vol 8 (33) ◽  
pp. 21145-21154 ◽  
Author(s):  
Eugene Lih ◽  
Ki Wan Park ◽  
So Young Chun ◽  
Hyuncheol Kim ◽  
Tae Gyun Kwon ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tissue Regeneration ◽  
Kidney Tissue ◽  
Decellularized Extracellular Matrix

Extracellular matrix scaffold-immune microenvironment modulates tissue regeneration

2021 ◽  
pp. 109524
Author(s):  
Yaling Yu ◽  
Weiying Zhang ◽  
Xuanzhe Liu ◽  
Hongshu Wang ◽  
Junjie Shen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tissue Regeneration ◽  
Immune Microenvironment ◽  
Extracellular Matrix Scaffold

Scaffold-supported extracellular matrices preserved by magnesium hydroxide nanoparticles for renal tissue regeneration

2020 ◽  
Vol 8 (19) ◽  
pp. 5427-5440 ◽  
Author(s):  
Yun Ah Kim ◽  
So Young Chun ◽  
Sung-Bin Park ◽  
Eunyoung Kang ◽  
Won-Gun Koh ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tissue Regeneration ◽  
Magnesium Hydroxide ◽  
Renal Tissue ◽  
Extracellular Matrices ◽  
Magnesium Hydroxide Nanoparticles

Fibroblast-derived extracellular matrix-supported scaffolds made up of PLGA were prepared with the enhanced preservation of ECM components by composites with magnesium hydroxide nanoparticles, and were applied for renal tissue regeneration.

Mass Production of Nanofibrous Extracellular Matrix with Controlled 3D Morphology for Large-Scale Soft Tissue Regeneration

2013 ◽  
Vol 19 (6) ◽  
pp. 458-472 ◽  
Author(s):  
Mohammad A. Alamein ◽  
Sebastien Stephens ◽  
Qin Liu ◽  
Stuart Skabo ◽  
Patrick H. Warnke
Keyword(s):  
Extracellular Matrix ◽  
Soft Tissue ◽  
Tissue Regeneration ◽  
Mass Production ◽  
Large Scale ◽  
Soft Tissue Regeneration ◽  
3D Morphology

scholarly journals Simulation of ECM with silk and chitosan nanocomposite materials

2017 ◽  
Vol 5 (24) ◽  
pp. 4789-4796 ◽  
Author(s):  
Z. Z. Ding ◽  
J. Ma ◽  
W. He ◽  
Z. L. Ge ◽  
Q. Lu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tissue Regeneration ◽  
Nanocomposite Materials

Extracellular matrix (ECM) is a system used to model the design of biomaterial matrices for tissue regeneration.

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