Contribution to the Optimization of a Gas Chromatographic Method by QbD Approach used for Analysis of Essential Oils from Salvia officinalis

2019 ◽  
Vol 70 (6) ◽  
pp. 2015-2020
Author(s):  
Silvia Robu ◽  
Aurelia Romila ◽  
Olimpia Dumitriu Buzia ◽  
Adrian Florin Spac ◽  
Camelia Diaconu ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Plant Material ◽  
Mobile Phase ◽  
Chromatographic Method ◽  
Salvia Officinalis ◽  
Salvia Officinalis L ◽  
Gas Chromatographic Method

Numerous articles on Salvia officinalis L. have been published regarding the composition of their essential oil. The considerable variation found may be due to the quality of the plant material as well as to the methods used for analysis. A simple GC-MS method was developed and optimized in the QbD approach, for the determination of sage essential oils. The optimization of GC-MS analysis was performed using different mobile phase flows, injection volumes, split ratios and temperature programs. The optimized method proved to be simple and can be successfully applied for the determination of sage essential oils.

scholarly journals The possibilities of the application of some species of sage (Salvia L) as auxiliaries in the treatment of some diseases

2003 ◽  
Vol 68 (6) ◽  
pp. 435-445 ◽  
Author(s):  
Ana Velickovic ◽  
Mihailo Ristic ◽  
Dragan Velickovic ◽  
Stevan Ilic ◽  
Natasa Mitic
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Plant Species ◽  
Salvia Officinalis ◽  
Diffusion Method ◽  
Pharmacological Effects ◽  
Ms Analysis ◽  
Salvia Officinalis L

The chemical composition of four essential oils of four species of sage (Salvia officinalis L., Salvia pratensis L., Salvia glutinosa L. and Salvia aethiopis L) are examined by GC-FID and GC-MS analysis. The presence of some components in the essential oil (mono- and sesquiterpene) determines the pharmacological effects and therapeutical application of a plant species. Salvia officinalis L. gives the highest yield of oil (1.1 %), while the lowest is in Salvia pratensis L. (0.1 %). The investigations included the determination of the antimicrobic activities of the essential oils by the diffusion method and the oil of Salvia pratensis L. proved to have the highest activity.

Identification and Determination of the Components of Garden Sage (Salvia officinalis L.) Essential Oil, Isolated by Different Extraction Methods

2020 ◽  
Vol 75 (11) ◽  
pp. 1451-1460
Author(s):  
Z. A. Temerdashev ◽  
V. V. Milevskaya ◽  
L. P. Ryabokon’ ◽  
N. N. Latin ◽  
N. V. Kiseleva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Extraction Methods ◽  
Salvia Officinalis ◽  
Salvia Officinalis L

scholarly journals A study of anti fungal activity of a combination of essential oils from medical herbs against water molds

2016 ◽  
Vol 13 (4) ◽  
pp. 674-680
Author(s):  
Baghdad Science Journal
Keyword(s):  
Essential Oils ◽  
Common Carp ◽  
Hplc Analysis ◽  
Salvia Officinalis ◽  
Active Compounds ◽  
Fungal Activity ◽  
Salvia Officinalis L ◽  
Anti Fungal

The aim of this study is to evaluate the anti fungal activity of a combination of essential oils against water molds. HPLC analysis was done to evaluate the quantity and quality of the active compounds in this combination which extracted from three herbs( Peppermint Menthapiperita ,Thyme Thymusvulgaris, Common sage Salvia officinalis L.) and the active compounds are Camphor,Menthol,,Thujone and Thymol with different concentrations. In this study (MIC) , (MFC) were measured and (LD50) determined after 48,96 h from fingerlings treatment of common carp in aquariums .The results of (MIC) were 0.025µl/ml for Aphanomyces sp. and 0.015µl/ml for both Achlya sp. and Fusariumsolani which showed significant differences(p

A Radio-Gas Chromatographic Method for Determining the Specific Radioactivity of Glycolic Acid in 14C-Labeled Leaf Tissue

1975 ◽  
Vol 53 (5) ◽  
pp. 609-614 ◽  
Author(s):  
J. D. Mahon ◽  
K. Egle ◽  
H. Fock
Keyword(s):  
Quantitative Determination ◽  
Plant Material ◽  
Glycolic Acid ◽  
Chromatographic Method ◽  
Specific Radioactivity ◽  
Leaf Tissue ◽  
Acid Solutions ◽  
Complete Procedure ◽  
Gas Chromatographic Method

A method for the extraction and quantitative determination of both the mass and radioactivity of glycolic acid from 14C-labeled leaf tissue is described. The recoveries of both mass and radioactivity from standard [1-14C]glycolic acid solutions averaged 98%, and recovery of radioactivity added to plant samples as [1-14C]glycolic acid was over 90% after the complete procedure. The method was reliable with total samples containing as little as 130 nmol of glycolic acid.The mass of glycolic acid recovered from sunflower leaf tissue was proportional to the amount of tissue extracted. In experiments with different plant material, the amount of glycolic acid varied between 530 and 1120 nmol/dm2 of leaf tissue. The specific radioactivity of the glycolic acid in sunflower leaf tissue during photosynthesis in 14CO2 was never more than 20% of the specific radioactivity of the 14CO2 supplied.

scholarly journals Determination of essential oil content in caraway (Carum carvi L.) species by means of supercritical fluid extraction

2011 ◽  
Vol 49 (No. 6) ◽  
pp. 277-282 ◽  
Author(s):  
J. Sedláková ◽  
B. Kocourková ◽  
L. Lojková ◽  
V. Kubáň
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Small Samples ◽  
Fluid Extraction ◽  
Carum Carvi ◽  
Main Portion

Dependently on planting conditions caraway fruits contain 1–9% of essential oils consisting of about 30 compounds. Carvone and limonene account for the main portion, about 95%. To evaluate the quality of various registered caraway (Carum carvi L.) cultivars (Kepron, Prochan and Rekord) planted during 1998–2000, regarding the effect of sample grinding and preparation, plant treatment and time of harvest, the amounts of essential oil and the carvone/limonene ratio were determined. Both whole and ground caraway seeds were extracted. As obvious from the results, SFE is not suitable for the determination of essential oils in whole seeds since the results are lower in comparison with those of ground caraway. The way of grinding was also examined. Of the three mills used, a splintery mill VIPO seemed to be the most suitable. Further, the amount of essential oil was studied in caraway gathered at the beginning of maturation (sample A) and at full ripeness (sample B) of caraway seeds. It was found that the samples gathered at full ripeness (samples B) had more essential oil. It was also concluded that the use of the regulator Roundup Bioaktiv during caraway maturation to unite the ripening of achenes in the main umbel and the first-order umbels, and the use of fungicides (Alert S and Prelude 10) affected positively the amount of essential oil in caraway. Possibilities of SFE application for the essential oil determination in small samples gathered during breeding were investigated. The results were used as a one of the criteria during breeding. Classical way of the essential oil determination does not allow this option. An alternative method for the isolation and determination of essential oils – supercritical fluid extraction (SFE) – was investigated in this study.

scholarly journals Spraying of swine buildings with lemon grass (Cymbopogon citratus) essential oil does not produce blood absorption in swine

2016 ◽  
Vol 8 (3) ◽  
pp. 360
Author(s):  
Seung-Jin Lee ◽  
Ki-Hyun Kim ◽  
Abraham Fikru Mechesso ◽  
Na-Hye Park ◽  
Seung-Chun Park
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Mobile Phase ◽  
Hplc Method ◽  
Detectable Level ◽  
Major Constituent ◽  
Cymbopogon Citratus ◽  
Blood Samples ◽  
Lemon Grass

<p align="left"><em>Cymbopogon citratus </em>(Lemon grass)<em> </em>essential oils have been used in swine buildings to reduce the offensive odor emanating from swine buildings. The present study was designed to investigate plasma residues of citral which is a major constituent of the essential oils of <em>Cymbopogon citratus. </em></p><p align="left">An HPLC method was established, validated and used for the determination of citral in swine plasma harvested from blood samples taken at the 14<sup>th</sup>, 21<sup>st</sup> and 28<sup>th</sup> day after spraying swine buildings with the 3% Lemon grass essential oil. Thereafter, analysis of the sample was<em> </em>conducted<em> </em>using HP ODS Hypersil column (200 × 4.6 mm, 5 µm) with a mobile phase consisting of methanol and 0.5% acetic acid; and a flow rate of 1mL/ min. The method was validated for parameters such as accuracy, precision, linearity and detection limits. Plasma spiked with standard citral (95%) revealed two chromatograms with retention times of about 10.7 and 12.2 minutes. The calibration curves for the citral isomers were found to be linear in the tested concentration ranges and mean recoveries wre 101% and 99.17%. This method was used to determine the residues of citral in swine plasma pretreated with methanol.<em> </em>Surprisingly, there was no any detectable level of citral in swine plasma within the 28 days of exposure.</p><p align="left">spraying of swine buildings with essential oils of lemon grass oil does not cause plasma residues of citral.</p><p align="left"> </p>

scholarly journals Essential Oils Chemical Variability of Seven Populations of Salvia Officinalis L. In North of Albania

2020 ◽  
Vol 39 (1) ◽  
pp. 31
Author(s):  
Alban Ibraliu ◽  
Adrian Doko ◽  
Avni Hajdari ◽  
Nazim Gruda ◽  
Zlatko Šatović ◽  
...  
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Oil Content ◽  
Salvia Officinalis ◽  
The North ◽  
Ms Analysis ◽  
Chemical Variability ◽  
Salvia Officinalis L

Essential oils from seven populations of Dalmatian sage from the North of Albania were extracted and analyzed according European Pharmacopeia 7.0. The yield differed from 1.95 to 2.75% v/w. The GC-FID-MS analysis of the sage oil revealed 42 constituents. Camphor (20.50-29.74%), followed by α-thujone (19.87-24.29%), 1,8-cineole (6.88-12.64%) and β-thujone (4.00-9.14%) were predominant constituents in all analyzed populations, representing 55.70-68.00% of the total components. All of the analyzed sage populations from northern Albania comply with ISO 6571/2008 standard and European Pharmacopeia 7.0. for Dalmatian sage essential oil content, while only four populations met the requirements of the ISO 9909/1997 standard for Dalmatian sage essential oil’s composition.

scholarly journals Comparison of antioxidant and antimicrobial activities of extracts obtained from Salvia glutinosa L. and Salvia officinalis L.

2011 ◽  
Vol 65 (5) ◽  
pp. 599-605 ◽  
Author(s):  
Dragan Velickovic ◽  
Ivana Karabegovic ◽  
Sasa Stojicevic ◽  
Miodrag Lazic ◽  
Valentina Marinkovic ◽  
...  
Keyword(s):  
Essential Oil ◽  
Plant Material ◽  
Aqueous Ethanol ◽  
Raw Materials ◽  
Antimicrobial Activities ◽  
Salvia Officinalis ◽  
Plant Materials ◽  
Salvia Officinalis L ◽  
Extracting Solvent ◽  
Antioxidant And Antimicrobial Activities

Antioxidant and antimicrobial activities as well as total phenols and flavonoids contents of Salvia glutinosa L. (glutinous sage) and Salvia officinalis L. (sage) extracts were studied. Methanol and aqueous ethanol (70% v/v) were used for extraction of bioactive compounds, both in the presence and the absence of ultrasound, from herb and the spent plant material remaining after the essential oil hydrodistillation. The ratio of plant material to extracting solvent was 1:10 g/ml. Antioxidant and antimicrobial activities of the extracts were found to depend on the type of plant material and the extraction conditions. The plant materials from which essential oil had been recovered were proven to be valuable raw materials for making various herbal preparations.

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